Protective Effect of Graviola (Annona muricata) Leaves Extract Against Cadmium-Induced Testicular Toxicity In Male Albino Rats

Abstract

Graviola (Annona muricata), an edible plant belonging to the Annonaceae family, has a long history in traditional medicine due to its wide range of therapeutic effects on many ailments. This study was planned to assess the protective potentials of Graviola leaves extract (GLE) on cadmiuminduced testicular damage in male albino rats. The study was conducted on 32 male adult Wistar rats randomly divided into four groups (n=8/each). A (control group); B (GLE, 100 mg/kg); C (cadmium chloride (CdCl 2), 5 mg/kg) and D (GLE, 100 mg / kg + CdCl 2 5 mg/kg). All administrations were performed by daily gavage for 8 weeks. Blood, testes, and epididymis were harvested for biochemical and histological investigations. In addition to sperm analysis, sperm DNA comet assay, and apoptotic indices. Results revealed that GLE significantly reversed (p<0.05) testicular oxidative stress induced by CdCl 2 , maintained male sex hormones levels, and restored testis architecture via increasing the testicular antioxidant status. GLE co-treatment significantly increased (p<0.05) sperm count, motility, viability, and DNA integrity along with significantly reducing sperm DNA damage and sperm head abnormality in comparison with CdCl 2 group. Additionally, GLE protected germ cells from Cd-induced apoptosis. In conclusion, results of the present study confirmed that GLE has potent antioxidant potentials and cytoprotective capacity against the deleterious effects of CdCl 2 on testicular tissue by reducing cellular oxidative stress prompted by the generation of the reactive oxygen species.

The impact of Annona muricata and metformin on semen quality and hormonal profile in Arsenic trioxide-induced testicular dysfunction in male Wistar rat

“…Onyegeme-Okerenta et al(126) and Olowofolahan et al(127) reported a significant decline in FSH level following Annona muricata in reproductive dysfunction, which disagree with the study outcome. The study corroborates the findings of Abd El-Monem and Elwakeel(128) and El-Sawi et al(129), revealing a significantly higher level of FSH following Annona muricata extract against testicular toxicity.…”

Annona squamosa Fruit Extract Ameliorates Lead Acetate-Induced Testicular Injury by Modulating JAK-1/STAT-3/SOCS-1 Signaling in Male Rats

“…A previous study indicated that A. squamosa had broad-spectrum activity against bacterial pathogens, which suggested that it could be used as a food sanitizer [19]. Leaf extract from another member of the Annonaceae family, A. muricata, has been studied for its ability to shield male rats from testicular damage caused by cadmium [20]. It has been reported that A. muricata attenuated testicular oxidative stress in rats [21].…”