Protective effect of curcumin in transgenic Drosophila melanogaster model of Parkinson’s disease

Siddique, Yasir Hasan; Ara, Gulshan; Jyoti, Smita; Afzal, Muhammad

doi:10.4081/ams.2012.e3

Cited by 28 publications

(20 citation statements)

References 28 publications

(26 reference statements)

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“…The efficacy of levodopa declines as PD progresses, but the oxidative stress can be reduced by agents having the free radical scavenging potential [50]. Our earlier studies with nordihydroguaiaretic acid [51], curcumin [52], capsaicin [53], and ascorbic acid [54] also showed a dose-dependent delay in the loss of climbing ability in the same PD model flies. The study with ascorbic acid, a well known antioxidant that showed no significant difference in the protein levels in the brain of PD model fly, suggests that only free radical scavenging activity is involved in the protection against the PD symptoms [54].…”

Section: Discussionmentioning

confidence: 99%

Synthesis of Alginate-Curcumin Nanocomposite and Its Protective Role in TransgenicDrosophilaModel of Parkinson’s Disease

et al. 2013

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The genetic models in Drosophila provide a platform to understand the mechanism associated with degenerative diseases. The model for Parkinson's disease (PD) based on normal human alpha-synuclein (αS) expression was used in the present study. The aggregation of αS in brain leads to the formation of Lewy bodies and selective loss of dopaminergic neurons due to oxidative stress. Polyphenols generally have the reduced oral bioavailability, increased metabolic turnover, and lower permeability through the blood brain barrier. In the present study, the effect of synthesized alginate-curcumin nanocomposite was studied on the climbing ability of the PD model flies, lipid peroxidation, and apoptosis in the brain of PD model flies. The alginate-curcumin nanocomposite at final doses of 10−5, 10−3, and 10−1 g/mL was supplemented with diet, and the flies were allowed to feed for 24 days. A significant dose-dependent delay in the loss of climbing ability and reduction in the oxidative stress and apoptosis in the brain of PD model flies were observed. The results suggest that alginate-curcumin nanocomposite is potent in delaying the climbing disability of PD model flies and also reduced the oxidative stress as well as apoptosis in the brain of PD model flies.

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Section: Discussionmentioning

confidence: 99%

Synthesis of Alginate-Curcumin Nanocomposite and Its Protective Role in TransgenicDrosophilaModel of Parkinson’s Disease

et al. 2013

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“…Crosses were set up as described in earlier published work [12]. The PD flies were exposed separately to different doses of curcumin (Sigma Aldrich, CAS 458-37-7) and mixed in culture medium at final concentration of 25, 50, and 100 μ M. The PD flies were also exposed to 10 −3 M of L-dopamine.…”

Section: Methodsmentioning

confidence: 99%

Effect of Curcumin on Lifespan, Activity Pattern, Oxidative Stress, and Apoptosis in the Brains of TransgenicDrosophilaModel of Parkinson’s Disease

Siddique

Naz

Jyoti

2014

BioMed Research International

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Background. A time dependent loss of dopaminergic neurons and the formation of intracellular aggregates of alpha synuclein have been reported in PD model flies. Methods. The progeny (PD flies) expressing human alpha synuclein was exposed to 25, 50, and 100 µM of curcumin mixed in the diet for 24 days. The effect of curcumin was studied on lifespan, activity pattern, oxidative stress, and apoptosis in the brains of PD model flies. The activity of PD model flies was monitored by using Drosophila activity monitors (DAMs). For the estimation of oxidative stress, lipid peroxidation and protein carbonyl content were estimated in the flies brains of each treated groups. The cell death in Drosophila brain was analyzed by isolating brains in Ringer's solution placing them in 70% ethanol and stained in acridine orange to calculate the gray scale values. Results. The exposure of flies to 25, 50, and 100 µM of curcumin showed a dose dependent significant delay in the loss of activity pattern, reduction in the oxidative stress and apoptosis, and increase in the life span of PD model flies. Conclusion. Curcumin is potent in reducing PD symptoms.

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“…The flies were cultured on standard Drosophila food containing agar, corn meal, sugar, and yeast at 25°C (24 ± 1) [ 10 ]. Crosses were set up as described in our earlier published work [ 11 ]. First, the climbing assay was performed for the PD flies and UAS-Hsap/SNCA.F (control).…”

Section: Methodsmentioning

confidence: 99%

Effect ofCentella asiaticaLeaf Extract on the Dietary Supplementation in TransgenicDrosophilaModel of Parkinson’s Disease

Siddique

Naz

Jyoti

et al. 2014

Parkinson's Disease

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The role of Centella asiatica L. leaf extract was studied on the transgenic Drosophila model flies expressing normal human alpha synuclein (h-αS) in the neurons. The leaf extract was prepared in acetone and was subjected to GC-MS analysis. C. asiatica extract at final concentration of 0.25, 0.50, and 1.0 μL/mL was mixed with the diet and the flies were allowed feeding on it for 24 days. The effect of extract was studied on the climbing ability, activity pattern, lipid peroxidation, protein carbonyl content, glutathione content, and glutathione-S-transferase activity in the brains of transgenic Drosophila. The exposure of extract to PD model flies results in a significant delay in the loss of climbing ability and activity pattern and reduced the oxidative stress (P < 0.05) in the brains of PD flies as compared to untreated PD flies. The results suggest that C. asiatica leaf extract is potent in reducing the PD symptoms in transgenic Drosophila model of Parkinson's disease.

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Protective effect of curcumin in transgenic Drosophila melanogaster model of Parkinson’s disease

Cited by 28 publications

References 28 publications

Synthesis of Alginate-Curcumin Nanocomposite and Its Protective Role in TransgenicDrosophilaModel of Parkinson’s Disease

Synthesis of Alginate-Curcumin Nanocomposite and Its Protective Role in TransgenicDrosophilaModel of Parkinson’s Disease

Effect of Curcumin on Lifespan, Activity Pattern, Oxidative Stress, and Apoptosis in the Brains of TransgenicDrosophilaModel of Parkinson’s Disease

Effect ofCentella asiaticaLeaf Extract on the Dietary Supplementation in TransgenicDrosophilaModel of Parkinson’s Disease

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