Protective effect of crocetin from Crocus sativus L. on myocardial ischemia-reperfusion injury in rats

Abstract

This study investigated the protective effect of crocetin from Crocus sativus L. on myocardial ischemia-reperfusion injury (MORO) in rats. Sixty SD rats were randomly divided into sham-operated, model, and low-, medium-and high-dose crocetin groups. Later 3 groups were intragastrically administrated with 10, 20 and 40 mg/kg crocetin from Crocus sativus L., respectively, for 1 week. Dn the 8th day, the MORO model was established in the later 4 groups. The blood biochemical indexes, hemodynamic indexes, myocardial infarct size, myocardial antioxidant indexes and myocardial expressions of B-cell lymphoma-2 (Bcl-2), Bcl-2 associated X protein (Bax), serine/threonine kinase (Akt) and phosphorylated Akt (p-Akt) protein were determined. Results showed that, compared with model group, in high-dose crocetin group the left ventricular systolic pressure, +dp/dt max and-dp/dt max , myocardial superoxide dismutase and glutathione peroxidase levels, and myocardial Bcl-2/Bax ratio and p-Akt/Akt ratio were significantly increased, and the left ventricular end diastolic pressure, myocardial infarct size, serum lactate dehydrogenase, creatine kinase-MB, cardiac troponin O, tumor necrosis factor-α, interleukin-1β, interleukin-6 and malondialdehyde levels were significantly decreased (all P < 0.05). On conclusion, crocetin from Crocus sativus L. has protective effects on MORO in rats.

Cardio-protective and Anti-atherosclerosis Effect of Crocetin on Vitamin D3 and HFD-induced Atherosclerosis in Rats

“…Natural substances have become increasingly popular as antioxidants and functional nutrients recently. Crocetin CT , a carotenoid phytoconstituent isolated from the saffron Crocus sativa or Gardenia jasminoides Ellis 19,20 . Crocetin commonly used as natural food colorant and spice in various parts of the world.…”

“…Recent years, crocetin showed antioxidant effects to counter the free radicals or oxidative stress and demonstrated anti-inflammatory effects on various animal models 21,22 . CT exert various types of pharmacological effects such as anti-apoptotic, anticancer, heart disease, neuroprotective, and anti-inflammatory effects 19,20,23,24 . This effect of CT, especially its capacity to suppress inflammation and oxidative stress, proposes that might be an important candidate to attenuate atherosclerosis.…”

Crocetin attenuating Urinary tract Infection and adherence of uropathogenic E. coli in NRK‐52E cells via an inflammatory pathway

“…Crocetin is the major phytoconstituents of Crocus sativus . It is a plant apocarotenoid with broad biologic potential, such as immunoregulation, antibacterial, anti‐inflammatory, and so on (Li et al., 2018; Liu et al., 2019; Sapanidou et al., 2016; Zhang et al., 2020). Previous research suggests that crocetin has been used against multiple inflammatory diseases, viz., ischemia‐reperfusion and has attained significant effectiveness (Farkhondeh et al., 2018; Li et al., 2018; Liu et al., 2019; Sapanidou et al., 2016; Zhang et al., 2020).…”

“…It is a plant apocarotenoid with broad biologic potential, such as immunoregulation, antibacterial, anti‐inflammatory, and so on (Li et al., 2018; Liu et al., 2019; Sapanidou et al., 2016; Zhang et al., 2020). Previous research suggests that crocetin has been used against multiple inflammatory diseases, viz., ischemia‐reperfusion and has attained significant effectiveness (Farkhondeh et al., 2018; Li et al., 2018; Liu et al., 2019; Sapanidou et al., 2016; Zhang et al., 2020). Crocetin showed potent antibacterial, antimicrobial, antioxidant, and anti‐inflammatory effects (Liu et al., 2019; Niska et al., 2015; Zhang et al., 2020; Zhou et al., 2015).…”

“…Previous research suggests that crocetin has been used against multiple inflammatory diseases, viz., ischemia‐reperfusion and has attained significant effectiveness (Farkhondeh et al., 2018; Li et al., 2018; Liu et al., 2019; Sapanidou et al., 2016; Zhang et al., 2020). Crocetin showed potent antibacterial, antimicrobial, antioxidant, and anti‐inflammatory effects (Liu et al., 2019; Niska et al., 2015; Zhang et al., 2020; Zhou et al., 2015). Still, to my knowledge, no one report showed the potential effect of crocetin on UTI.…”