2017
DOI: 10.18632/aging.101226
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Abstract: The core mechanism of Late-onset hypogonadism (LOH) is the deficiency of androgen due to the functional and quantitative decline of testicular Leydig cells. Here we explored the protective effect of calretinin, a Ca2+-binding protein, on Leydig cells. We found in MLTC-1 cells transfected with LV-calb2, the cell viability and optical density (OD) were higher (p<0.05), cells in the S phase of the cell cycle were increased (p<0.01) and p-ERK1/2 and p-AKT levels were significantly higher (p<0.01 and p<0.05), while… Show more

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Cited by 8 publications
(40 citation statements)
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References 39 publications
(40 reference statements)
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“…Male late-onset hypogonadism (LOH) is a clinical and biochemical syndrome associated with advancing age. It is characterized by a deficiency in serum testosterone levels and symptoms such as low sexual desire, erectile dysfunction, muscle mass loss, obesity, osteoporosis, and depression [1][2][3]. LOH reportedly affects 7-30% of aged males [4] and may be a significant detriment to their quality of life, adversely affecting the function of multiple organ systems [5].…”
Section: Introductionmentioning
confidence: 99%
“…Male late-onset hypogonadism (LOH) is a clinical and biochemical syndrome associated with advancing age. It is characterized by a deficiency in serum testosterone levels and symptoms such as low sexual desire, erectile dysfunction, muscle mass loss, obesity, osteoporosis, and depression [1][2][3]. LOH reportedly affects 7-30% of aged males [4] and may be a significant detriment to their quality of life, adversely affecting the function of multiple organ systems [5].…”
Section: Introductionmentioning
confidence: 99%
“…Since ovarian theca cells corresponds to testicular Leydig cells, at least in terms of androgen production, similar functions for calretinin in the ovary are likely, which would lend support to the first scenario where it is the theca cell numbers that are affected. Also, recent findings suggest that calretinin is involved in Leydig cell function by protecting against cell apoptosis (Xu et al 2017) and by promoting steroidogenesis (Xu et al 2018). Thus, the potential importance of calretinin in androgen producing cells, as well as the effects on primordial follicles and reproductive senescence seen in littermates exposed to anti-androgenic mixtures (Johansson et al 2016), indicates that calretinin could be a potential marker not only of anti-androgenic effect, but potentially also for late life ovarian dysgenesis.…”
Section: Discussionmentioning
confidence: 99%
“…A number of studies have previously demonstrated cell cycle arrest at the S phase upon treatment of Leydig cells with different compounds ( 36 38 ). A study recently investigated the effects of calretinin on the testicular Leydig cell line, MLTC-1, whereby cell cycle analysis demonstrated a significantly high cell number at the S phase, suggesting that the reduction in cell viability was due to S-phase cell cycle arrest ( 36 ).…”
Section: Discussionmentioning
confidence: 99%
“…A number of studies have previously demonstrated cell cycle arrest at the S phase upon treatment of Leydig cells with different compounds ( 36 38 ). A study recently investigated the effects of calretinin on the testicular Leydig cell line, MLTC-1, whereby cell cycle analysis demonstrated a significantly high cell number at the S phase, suggesting that the reduction in cell viability was due to S-phase cell cycle arrest ( 36 ). Similarly, a study conducted a cell cycle test by using primary Leydig cells treated with different concentrations of dehydroepiandrosterone (DHEA) and discovered that after 48 h 50 µM DHEA treatment, the cell number at S phase was significantly increased following a reduction in the cell number at the G 2 phase compared to that in the control group ( 37 ).…”
Section: Discussionmentioning
confidence: 99%