Protective effect of a polyphenols-rich extract from Inonotus Sanghuang on bleomycin-induced acute lung injury in mice

Su, Xing; K, Liu; Xie, Yu; Zhang, Mengdi; Wang, Yong; Zhao, Min; Guo, Yuanqiang; Zhang, Yijie; Wang, Junpeng

doi:10.1016/j.lfs.2019.05.074

Cited by 14 publications

(13 citation statements)

References 39 publications

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“…1 j). Infiltration of neutrophils and macrophages into the acutely injured lungs of mice after intratracheal exposure to BLM is well-described [ 27 , 28 ]. Thus, BAL cells were assessed using cytospins and Wright-Giemsa staining .…”

Section: Resultsmentioning

confidence: 99%

“…The mechanism of BLM-induced ALI is not fully understood, but numerous studies have shown that the exposure of lungs to BLM may directly trigger a series of oxidative reactions leading to cell death [ 27 , 28 ]. Alveolar epithelial cell injury and death are further aggravated by the infiltration of inflammatory cells into the lung [ 27 – 29 , 39 ].…”

Section: Discussionmentioning

confidence: 99%

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Intratracheal transplantation of trophoblast stem cells attenuates acute lung injury in mice

Han

Hou

et al. 2021

Stem Cell Res Ther

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Background Acute lung injury (ALI) is a common lung disorder that affects millions of people every year. The infiltration of inflammatory cells into the lungs and death of the alveolar epithelial cells are key factors to trigger a pathological cascade. Trophoblast stem cells (TSCs) are immune privileged, and demonstrate the capability of self-renewal and multipotency with differentiation into three germ layers. We hypothesized that intratracheal transplantation of TSCs may alleviate ALI. Methods ALI was induced by intratracheal delivery of bleomycin (BLM) in mice. After exposure to BLM, pre-labeled TSCs or fibroblasts (FBs) were intratracheally administered into the lungs. Analyses of the lungs were performed for inflammatory infiltrates, cell apoptosis, and engraftment of TSCs. Pro-inflammatory cytokines/chemokines of lung tissue and in bronchoalveolar lavage fluid (BALF) were also assessed. Results The lungs displayed a reduction in cellularity, with decreased CD45+ cells, and less thickening of the alveolar walls in ALI mice that received TSCs compared with ALI mice receiving PBS or FBs. TSCs decreased infiltration of neutrophils and macrophages, and the expression of interleukin (IL) 6, monocyte chemoattractant protein-1 (MCP-1) and keratinocyte-derived chemokine (KC) in the injured lungs. The levels of inflammatory cytokines in BALF, particularly IL-6, were decreased in ALI mice receiving TSCs, compared to ALI mice that received PBS or FBs. TSCs also significantly reduced BLM-induced apoptosis of alveolar epithelial cells in vitro and in vivo. Transplanted TSCs integrated into the alveolar walls and expressed aquaporin 5 and prosurfactant protein C, markers for alveolar epithelial type I and II cells, respectively. Conclusion Intratracheal transplantation of TSCs into the lungs of mice after acute exposure to BLM reduced pulmonary inflammation and cell death. Furthermore, TSCs engrafted into the alveolar walls to form alveolar epithelial type I and II cells. These data support the use of TSCs for the treatment of ALI.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Intratracheal transplantation of trophoblast stem cells attenuates acute lung injury in mice

Han

Hou

et al. 2021

Stem Cell Res Ther

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“…These data suggest that TSCs, a placenta-derived stem cell easily obtained from discarded tissue, can be a potential future therapeutic option for ALI. ALI in the early stage after exposure to BLM (within 7 days) is characterized by an extensive infiltration of inflammatory cells into the lung (40)(41)(42)48). When mice received TSCs intratracheally 3 days after BLM exposure, at day 7 there was a significant decrease in CD45 + cells compared with mice receiving PBS or FBs (Figure 1).…”

Section: Discussionmentioning

confidence: 99%

“…The mechanism of BLM-induced ALI is not fully understood, but numerous studies have shown that the exposure of lungs to BLM may directly trigger a series of oxidative reactions leading to cell death (40,41). Alveolar epithelial cell injury and death are further aggravated by the infiltration of inflammatory cells into the lung.…”

Section: Discussionmentioning

confidence: 99%

“…Intratracheal transplantation of TSCs significantly decreased the total cell counts in BALF, compared with ALI mice that received PBS or FBs (Figure 1c, left graph). Infiltration of neutrophils and macrophages into the acutely injured lungs of mice after intratracheal exposure to BLM is well described (40,41). Thus, BAL cells were assessed using cytospins and Wright-Giemsa staining.…”

Section: Transplantation Of Tscs Attenuates Inflammation In Alimentioning

confidence: 99%

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Intratracheal Transplantation of Trophoblast Stem Cells Attenuates Acute Lung Injury in Mice

Han

Hou

et al. 2020

Preprint

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BackgroundAcute lung injury (ALI) is a common lung disorder that affects millions of people every year. The infiltration of inflammatory cells into the lungs and death of the alveolar epithelial cells are key factors to trigger a pathological cascade. Trophoblast stem cells (TSCs) have been shown to possess immune privilege, immunomodulation, and the potential of self-renewal and multipotency with differentiation into three germ layers. We hypothesized that intratracheal transplantation of TSCs may alleviate ALI.MethodsALI was induced by intratracheal delivery of Bleomycin (BLM) in mice. At day 3 after exposure to BLM, pre-labeled TSCs or Fibroblasts (FBs) were intratracheally administered into the lungs. At day 7 after exposure to BLM, histological analyses of the lungs were performed for inflammatory infiltrates, cell apoptosis, and engraftment of TSCs. Pro-inflammatory cytokines/chemokines of lung tissue and in bronchoalveolar lavage fluid (BALF) were also assessed by quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assays , respectively. Flow cytometry was utilized to assess engraftment of TSCs and cell apoptosis.Results Histological analyses of the lungs displayed a reduction in cellularity and less thickening of the alveolar walls in ALI mice that received TSCs compared with ALI mice receiving PBS or FBs. TSCs decreased infiltration of neutrophils and macrophages, and the expression of interleukin 6 (IL-6), monocyte chemoattractant protein-1 (MCP-1) and keratinocyte-derived chemokine in the injured lungs. The levels of IL-6 and MCP-1 in BALF were decreased in ALI mice receiving TSCs, compared with ALI mice that received PBS or FBs. Terminal deoxynucleotidyl transferase dUTP nick end labeling confirmed that TSCs significantly reduced BLM-induced apoptosis of lung tissue in vivo and epithelial cells in vitro . Transplanted TSCs integrated into the alveolar walls and expressed aquaporin 5 and prosurfactant protein C, markers for alveolar epithelial type I and II cells, respectively. ConclusionIntratracheal transplantation of TSCs into the lungs of mice after acute exposure to BLM reduced pulmonary inflammation and cell death. Furthermore, TSCs engrafted into the alveolar walls to form alveolar epithelial type I and II cells. These data support the use of TSCs for the treatment of ALI.

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Edible bird's nest inhibits the inflammation and regulates the immunological balance of lung injury mice by SO₂

et al. 2022

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The purpose was to investigate effect of edible bird's nest (EBN) on the immunological properties of mice with lung injury induced by sulfur dioxide (SO 2 ). In mice, SO 2 exposure caused symptoms that included a cough, tiredness, and weight loss. Administering EBN improved the immunological function of mice with lung injury in a dosedependent manner, leading to recovery of the lung tissue and increasing thymus and spleen indices. IL-2 and IFN-γ in the serum by Th1 cells in response to EBN played a leading role in cellular immune regulation. Moreover, a medium dose of EBN regulated the immune stress response, inhibited apoptosis of splenic lymphocytes, and promoted cellular immunity. EBN inhibited production of IL-8 and MDA and upregulated mRNA expression of atrophin-1 and cyclooxygenase-2 in lung tissue. EBN attenuates the lung injury induced by SO 2 in mice through a mechanism that involves the inhibition of inflammation and regulation of immunological balance.

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Protective effect of a polyphenols-rich extract from Inonotus Sanghuang on bleomycin-induced acute lung injury in mice

Cited by 14 publications

References 39 publications

Intratracheal transplantation of trophoblast stem cells attenuates acute lung injury in mice

Intratracheal transplantation of trophoblast stem cells attenuates acute lung injury in mice

Intratracheal Transplantation of Trophoblast Stem Cells Attenuates Acute Lung Injury in Mice

Edible bird's nest inhibits the inflammation and regulates the immunological balance of lung injury mice by SO₂

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Protective effect of a polyphenols-rich extract from Inonotus Sanghuang on bleomycin-induced acute lung injury in mice

Cited by 14 publications

References 39 publications

Intratracheal transplantation of trophoblast stem cells attenuates acute lung injury in mice

Intratracheal transplantation of trophoblast stem cells attenuates acute lung injury in mice

Intratracheal Transplantation of Trophoblast Stem Cells Attenuates Acute Lung Injury in Mice

Edible bird's nest inhibits the inflammation and regulates the immunological balance of lung injury mice by SO2

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Edible bird's nest inhibits the inflammation and regulates the immunological balance of lung injury mice by SO₂