Protective barrier properties of Rhinosectan® spray (containing xyloglucan) on an organotypic 3D airway tissue model (MucilAir): results of an in vitro study

Servi, Barbara De; Ranzini, Francesco; Piqué, Núria

doi:10.1186/s13223-017-0209-6

Cited by 23 publications

(38 citation statements)

References 35 publications

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“…Pollen did not stimulate any cytokine release (IL-8, TNF-α and IL-6), which is based on a protective mucus layer, as indicated by the SEM images. Furthermore, the concentration of 10 µg/mL LPS might be further increased to stimulate the MucilAir™ system in order to generate a more pronounced response [29]. Nonetheless, in an inflammatory state the properties of mucus change and the native barrier might be less efficient, which is hard to mimic in vitro [30,31,32].…”

Section: Discussionmentioning

confidence: 99%

Combining MucilAir™ and Vitrocell® Powder Chamber for the In Vitro Evaluation of Nasal Ointments in the Context of Aerosolized Pollen

Metz

Knoth²,

Groß³

et al. 2018

Pharmaceutics

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Hay fever is notoriously triggered when nasal mucosa is exposed to allergenic pollen. One possibility to overcome this pollen exposure may be the application of an ointment with physical protective effects. In this context, we have investigated Bepanthen® Eye and Nose Ointment and the ointment basis petrolatum as reference while using contemporary in vitro techniques. Pollen from false ragweed (Iva xanthiifolia) was used as an allergy-causing model deposited as aerosol using the Vitrocell® Powder Chamber (VPC) on Transwell® inserts, while being coated with either Bepanthen® Eye and Nose Ointment and petrolatum. No pollen penetration into ointments was observed upon confocal scanning laser microscopy during an incubation period of 2 h at 37 °C. The cellular response was further investigated by integrating the MucilAir™ cell system in the VPC and by applying pollen to Bepanthen® Eye and Nose Ointment covered cell cultures. For comparison, MucilAir™ were stimulated by lipopolysaccharides (LPS). No increased cytokine release of IL-6, TNF-α, or IL-8 was found after 4 h of pollen exposure, which demonstrates the safety of such ointments. Since nasal ointments act as a physical barrier against pollen, such preparations might support the prevention and management of hay fever.

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Section: Discussionmentioning

confidence: 99%

Combining MucilAir™ and Vitrocell® Powder Chamber for the In Vitro Evaluation of Nasal Ointments in the Context of Aerosolized Pollen

Metz

Knoth²,

Groß³

et al. 2018

Pharmaceutics

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“…Different 3-D models, which mimic specific components or processes of the respiratory system, have been extensively used to test various novel anti-infective agents. For example, the protective role of nasal sprays in maintaining the barrier function of nasal epithelial cells has been shown in vitro using a commercially available 3-D nasal mucosal model (De Servi et al 2017). The ability of a novel antimicrobial peptide, derived from frog skin, to affect the permeability of lung epithelium has been shown using primary bronchial epithelial cells differentiated in vitro, where the barrier integrity was measured with an electrode determining the transepithelial electrical resistance (TEER) .…”

Section: Respiratory Modelsmentioning

confidence: 99%

Human Organotypic Models for Anti-infective Research

Hendriks¹,

Cruz²,

Soldaini³

et al. 2018

Current Topics in Microbiology and Immunology

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The use of human organotypic models for biomedical research is experiencing a significant increase due to their biological relevance, the possibility to perform high-throughput analyses, and their cost efficiency. In the field of anti-infective research, comprising the search for novel antipathogenic treatments including vaccines, efforts have been made to reduce the use of animal models. That is due to two main reasons: unreliability of data obtained with animal models and the increasing willingness to reduce the use of animals in research for ethical reasons. Human three-dimensional (3-D) models may substitute and/or complement in vivo studies, to increase the translational value of preclinical data. Here, we provide an overview of recent studies utilizing human organotypic models, resembling features of the cervix, intestine, lungs, brain, and skin in the context of anti-infective research. Furthermore, we focus on the future applications of human skin models and present methodological protocols to culture human skin equivalents and human skin explants.

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“…In recent years progress has been made in constructing 3D tissue models and using them for various research and clinical applications [18][19][20][21]. This allowed for departure from the use of animal tissue models, and for obtaining data in realistic physiologic conditions [21][22][23][24][25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Incoming Flow Turbulence Reduction Increases the Efficiency of Aerosolized Medication Delivery to 3D Oral Mucosal Tissue Models.

Haroutunian

Tsagikian²,

Zheng

et al. 2020

Preprint

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Background: Inhalable medication devices on the market deliver aerosolized drugs in a turbulent flow, which creates significant obstacles for reaching the lower lungs. The complexity of the interaction of external turbulent flow from an inhalation device with complex anatomy of the upper airways, including the oropharyngeal cavity, makes the fidelity of aerosolized medication delivery to the lungs extremely low. Unpredictable outcomes, waste, and side effects result from unintended upper airway drug deposition. Methods: Here we compared the efficiency of aerosolized medication (fluticasone) delivery via a novel Flow Modification Device (ModiFlow) to that of a Standard Spacer (SS) device. The ability of ModiFlow to minimize the turbulence in the flow was assessed preliminarily by measuring the length of the Laminar Outflow using video recording. Oral mucosal 3D tissue culture (SkinAxis) was used as a target for delivery, and was placed either “well within” the range of the length of the oro-pharyngeal cavity (5cm) or “well outside” of it (20cm) from exit points of each device. The efficiency of fluticasone delivery to the surface of tissue cultures was quantified by mass spectrometry. Results: The results of the study demonstrated a statistically significant advantage of ModiFlow over a Standard Spacer in delivering aerosolized fluticasone to target tissue at both distances. The difference in the efficiency of delivery between the two spacers was more pronounced at a longer distance.Conclusions: Lamination of the outflow using internal septi helps improve the delivery of aerosolized medication to target tissue despite an increased inner surface area of the spacer device, which also indicates lesser deposition of the medication on the walls of the test tube. This suggests that the use of ModiFlow will potentially result in the more efficient delivery of aerosolized medications to the lungs with lesser deposition in the oral cavity and fewer side effects.

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Protective barrier properties of Rhinosectan® spray (containing xyloglucan) on an organotypic 3D airway tissue model (MucilAir): results of an in vitro study

Cited by 23 publications

References 35 publications

Combining MucilAir™ and Vitrocell® Powder Chamber for the In Vitro Evaluation of Nasal Ointments in the Context of Aerosolized Pollen

Combining MucilAir™ and Vitrocell® Powder Chamber for the In Vitro Evaluation of Nasal Ointments in the Context of Aerosolized Pollen

Human Organotypic Models for Anti-infective Research

Incoming Flow Turbulence Reduction Increases the Efficiency of Aerosolized Medication Delivery to 3D Oral Mucosal Tissue Models.

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