Protective and therapeutic effects of ethanolic extract of Nasturtium officinale (watercress) and vitamin E against bleomycin-induced pulmonary fibrosis in rats

Abstract

Background and purpose: Pulmonary fibrosis is a chronic disease of the lungs caused by inflammation, species of reactive oxygen, and immune defects. Antioxidant properties of Nasturtium officinale has been reported in some studies. Therefore, the objective of the current study was to evaluate the effect of ethanolic extract of Nasturtium officinale (EENO) on bleomycin (BLM)-induced lung fibrosis in rats. Experimental approach: Forty adult male Wistar rats (180-220 g) were randomly divided into 5 experimental groups. Normal control, BLM control received a single dose of BLM (6 IU/kg) intratracheally only on the first day, EENO + BLM group received EENO (500 mg/kg) one week before intratracheal BLM instillation and two weeks afterward, BLM + EENO group and BML + vitamin E group received EENO (500 mg/kg) and vitamin E (500 mg/kg) half-hour after BLM installation, respectively. The animals were sacrificed on day 22. Change in body weight, lung index, serum level of malondialdehyde (MDA) and nitric oxide (NO) metabolite, lung tissue hydroxyproline content and lung pathology were assessed. Findings/Results: Pre- or post-treatment with EENO attenuated pulmonary fibrosis as evidenced by normalized lung index, improved histological changes and inhibited collagen deposition (hydroxyproline) in the animal lung. EENO also decreased MDA and NO metabolite release in comparison to the BLM control. vitamin E (500 mg/ kg) also significantly inhibited the BLM-induced lung toxicity. Conclusions and implications: EENO can prevent BLM-induced lung fibrosis in rats via antioxidant activities. However, more studies are needed to elicit the exact mechanism of this effect.

The Hydroalcoholic Extract of Nasturtium officinale Reduces Lung Inflammation and Oxidative Stress in an Ovalbumin-Induced Rat Model of Asthma

“…In the present study, treatment with the NOE similar to DTX treatment significantly reduced overexpression of the α -SMA gene in OVA-induced asthmatic rats; also the NOE had a noticeable effect on the expression of the inflammatory gene TGF- β . In one study, the antioxidant and anti-inflammatory properties of NOE were suggested to protect against bleomycin-induced pulmonary fibrosis in a rat model [ 65 ]. Accordingly, several studies have reported anti-inflammatory, antioxidant, and immune-regulating effects of the NOE [ 60 , 61 ].…”

Stachys pilifera Benth. Ameliorates Bleomycin-Induced Pulmonary Fibrosis in Rats through the Antioxidant Pathways

“…Group I received a single intratracheal instillation of normal saline (control group) Group II I received a single intratracheal instillation of BLM solution (7.5 IU/kg in 0.9% NaCl) (BLM-treated group) Group III was given a single intratracheal instillation of BLM solution (7.5 IU/kg in 0.9% NaCl) and a daily SPHE (300 mg/kg, gavage) for two weeks (BLM/ PPX 0.5) Group IV received a single intratracheal instillation of BLM solution (7.5 IU/kg in 0.9% NaCl) and a daily SPHE (600 mg/kg, gavage) for two weeks (BLM/PPX 1) Group V was given a single intratracheal instillation of BLM solution (7.5 IU/kg in 0.9% NaCl) and a daily Vit E (500 mg/kg, gavage) for two weeks (BLM/Vit E) e number of animals and the dose of drugs were selected according to previous studies [20,24,25].…”

Protective Effects of Hydroalcoholic Extract of Rosa canina Fruit on Vancomycin-Induced Nephrotoxicity in Rats

“…Ripe RC fruit was collected in June 2017, from the suburbs of Yasuj, Iran, which was evaluated by Dr. A. Jafari (Botany Department, Natural Resource and Animal Husbandry Research Centre, Yasuj University, Yasuj, Iran) [ 27 ]. The seeds were cleaned, and dust and soil were removed by using distilled water.…”