Protective activity against oocyst shedding in cats vaccinated with crude rhoptry proteins of the Toxoplasma gondii by the intranasal route

Garcia, João Luís; Navarro, Italmar Teodorico; Biazzono, Luciane; Freire, Roberta Lemos; Guimarães, José da Silva; Cryssafidis, Andréas L.; Bugni, Felipe Monteiro; Cunha, Ivo Alexandre Leme da; Hamada, Fernando Nakanishi; Dias, Renata Cristina Ferreira

doi:10.1016/j.vetpar.2007.01.007

Cited by 32 publications

(26 citation statements)

References 29 publications

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“…Feces from each cat were collected daily from 1 st until 20 th day after challenge and examined microscopically for oocysts as described by Garcia et al (2007) and . Briefly, feces obtained over a period of 24 h were diluted in a small volume of distilled water; 1 g of this material were admixed with 10 mL of sucrose solution (specific gravity, 1.18), filtered, and centrifuged (1200 x g for 10 min).…”

Section: Measurements Of Oocyst Shedding By Sucrose Flotationmentioning

confidence: 99%

“…The risk of becoming infected depends on culinary habits, including the regular consumption of raw or undercooked meat and living in an environment with a higher risk of oocyst contamination (NAVARRO et al, 1992;DIAS et al, 2005;JONES et al, 2006;SANTOS et al, 2010;SILVA & LANGONI, 2016). Cats play an important role in this process due to their close interactions with human beings where infected cats shed millions of oocysts in their feces that subsequently contaminate the environment (DUBEY, 1995;GARCIA et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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rROP2 from Toxoplasma gondii as a potential vaccine against oocyst shedding in domestic cats

Zulpo

Igarashi

Sammi

et al. 2017

Rev. Bras. Parasitol. Vet.

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The aim of the present study was to evaluate oocyst shedding in cats immunized by nasal route with T. gondii proteins ROP2. Twelve short hair cats (Felis catus) were divided in three groups G1, G2 and G3 (n=4). Animals from G1 received 100 μg of rROP2 proteins plus 20 μg of Quil-A, G2 received 100 μg of BSA plus 20 μg of Quil-A, and the G3 only saline solution (control group). All treatments were done by intranasal route at days 0, 21, 42, and 63. The challenge was performed in all groups on day 70 with ≅ 800 tissue cysts of ME-49 strain by oral route. Animals from G1 shed less oocysts (86.7%) than control groups. ELISA was used to detect anti-rROP2 IgG and IgA, however, there were no correlation between number of oocyst shedding by either IgG or IgA antibody levels. In the present work, in spite of lesser oocysts production in immunized group than control groups, it was not possible to associate the use of rROP2 via nostrils with protection against oocyst shedding. For the future, the use of either other recombinant proteins or DNA vaccine, in combination with rROP2 could be tested to try improving the efficacy of this kind of vaccine.

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Section: Measurements Of Oocyst Shedding By Sucrose Flotationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

rROP2 from Toxoplasma gondii as a potential vaccine against oocyst shedding in domestic cats

Zulpo

Igarashi

Sammi

et al. 2017

Rev. Bras. Parasitol. Vet.

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“…In sharp contrast, sterile immunity against tissue cyst formation and oocyst shedding has been obtained in pigs (Kringel et al 2004, Garcia et al 2005, Jongert et al 2008a) and cats, respectively , Garcia et al 2007). The highest protection against congenital disease, however, was obtained in a rat model (Zenner et al 1999).…”

Section: Discussionmentioning

confidence: 99%

Vaccines against Toxoplasma gondii: challenges and opportunities

Jongert

Roberts

Gargano

et al. 2009

Mem. Inst. Oswaldo Cruz

170

130

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“…Récemment, des protéines purifiées, associées à un adjuvant, ont été administrées à des chats par voie nasale, entraînant une bonne protection contre l'excrétion d'oocystes, puisque deux chats sur trois testés n'en sécrétaient pas. Cependant, malgré ces bons résul-tats, ces études restent à confirmer car les effectifs par lot sont très faibles (trois chats/lot) (Garcia et al 2007). …”

Section: Les Vaccins Moléculairesunclassified

Vaccination contre la toxoplasmose chez les animaux de rente

Moiré¹,

Mévélec²,

Ducourneau³

et al. 2009

bavf

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La toxoplasmose est une zoonose mondialement répandue. Chez l'homme, elle apparaît après l'ingestion de viandes insuffisamment cuites d'animaux contaminés. Chez le mouton et la chèvre, elle est à l'origine de nombreux avortements. Une étude approfondie de sa séroprévalence chez les différentes espèces animales peut permettre de mieux informer les consommateurs et ainsi de limiter les risques de transmission. La vaccination des animaux semble être une alternative intéressante puisqu'elle pourrait diminuer la transmission à l'homme mais également prévenir les avortements chez les brebis. L'utilisation d'une souche naturelle de Toxoplasma gondii incomplète, présentant une virulence atténuée, a montré son efficacité dans la protection contre l'avortement des brebis. Cependant, sa virulence n'est pas bien contrôlée, et le risque de réversion vers la forme virulente existe. Les techniques de biologie moléculaire ont permis d'obtenir des souches atténuées par la délétion de gènes ciblés, qui ne sont pas susceptibles de retrouver leur virulence d'origine. L'une d'elles, appelée Mic1-3KO, a montré son efficacité dans un modèle murin contre la toxoplasmose chronique et congéni-tale. Elle est également efficace contre la toxoplasmose congénitale chez la brebis. Cette démarche vaccinale reste prometteuse. De plus, l'utilisation du toxoplasme comme vecteur vaccinal reste une perspective intéressante, puisque ce parasite est capable d'exprimer des protéines étrangères. Mots-clés :

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Protective activity against oocyst shedding in cats vaccinated with crude rhoptry proteins of the Toxoplasma gondii by the intranasal route

Cited by 32 publications

References 29 publications

rROP2 from Toxoplasma gondii as a potential vaccine against oocyst shedding in domestic cats

rROP2 from Toxoplasma gondii as a potential vaccine against oocyst shedding in domestic cats

Vaccines against Toxoplasma gondii: challenges and opportunities

Vaccination contre la toxoplasmose chez les animaux de rente

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