M Riviere, J Tartaglia, M E Perkus, E K Norton, C M Bongermino, F Lacoste, C Duret, P Desmettre, E Paoletti
Abstract:Glycoproteins gp5O, gll, and glll of pseudorabies virus (PRV) were expressed either individually or in combination by vaccinia virus recombinants. In vitro analysis by immunoprecipitation and immunofluorescence demonstrated the expression of a gll protein of approximately 120 kDa that was proteolytically processed to the glIb (67to 74-kDa) and glIc (58-kDa) mature protein species similar to those observed in PRV-infected cells. Additionally, the proper expression of the 90-kDa gIll and 50-kDa gp5O was observed…
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