Protection of cattle from bovine tuberculosis by vaccination with BCG by the respiratory or subcutaneous route, but not by vaccination with killed Mycobacterium vaccae

Buddle, Bryce M.; Keen, D.L.; Thomson, A.J.; Jowett, G. H.; McCarthy, Allison R.; Heslop, J; Lisle, Geoffrey W. de; Stanford, J. L.; Aldwell, Frank E.

doi:10.1016/0034-5288(95)90023-3

Cited by 137 publications

(103 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In adoptive transfer experiments, however, Orme and Collins (25) demonstrated that aerogenic immunization with BCG offered no protective advantage over s.c. immunization in mice. This finding has also been supported in other animal models of mycobacterial infections, such as possums (1) and cattle (6).…”

Section: Discussionsupporting

confidence: 69%

Lymphocyte Recruitment and Protective Efficacy against Pulmonary Mycobacterial Infection Are Independent of the Route of PriorMycobacterium bovisBCG Immunization

et al. 2002

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 69%

Lymphocyte Recruitment and Protective Efficacy against Pulmonary Mycobacterial Infection Are Independent of the Route of PriorMycobacterium bovisBCG Immunization

et al. 2002

View full text Add to dashboard Cite

show abstract

“…Currently, promising vaccines against bovine TB are based on heterologous prime-boost combinations that include the live attenuated M. bovis vaccine strain bacille Calmette-Guérin (BCG) as one of their components (8). However, as for humans, the vaccination of cattle with BCG compromises the specificity of the tuberculin skin test since PPD contains crossreactive antigens shared by both pathogenic and vaccine strains (4,5,26). Therefore, the development of diagnostic tests for differentiating vaccinated from infected animals (DIVA) is an essential prerequisite to allow the inclusion of BCG-based vaccination as part of bovine TB control strategies.…”

mentioning

confidence: 99%

Development of a Skin Test for Bovine Tuberculosis for Differentiating Infected from Vaccinated Animals

Whelan¹,

Clifford²,

Upadhyay³

et al. 2010

J Clin Microbiol

View full text Add to dashboard Cite

The tuberculin skin test has been used for the diagnosis of bovine and human tuberculosis (TB) for over a hundred years. However, the specificity of the test is compromised by vaccination with the Mycobacterium bovis-derived vaccine strain bacille Calmette-Guérin (BCG). Since current promising vaccines against bovine TB are based on heterologous prime-boost combinations that include BCG, there is a need for diagnostic tests for differentiating infected from vaccinated animals (DIVA). The application of antigens such as ESAT-6 and CFP-10 for DIVA has so far been realized largely through their application in the blood-based gamma interferon release assay. In the current study, we have reassessed the potential of such antigens as skin test reagents for DIVA in cattle. A cocktail of the Mycobacterium tuberculosis complex recombinant protein antigens ESAT-6, CFP-10, MPB70, and MPB83 elicited delayed-type hypersensitivity (DTH) skin test responses in 78% of naturally infected tuberculin-positive cattle. Importantly, this cocktail induced no skin responses in BCGvaccinated cattle despite them being sensitized for strong tuberculin responses. Further optimization of skin test antigen combinations identified that the inclusion of Rv3615c (Mb3645c) enhanced skin test sensitivity in naturally infected cattle without compromising specificity. In addition, we demonstrate for the first time the utility of synthetic peptides as promising skin test antigens for bovine TB for DIVA. Our data provide a promising basis for the future development of skin tests for DIVA with practical relevance for TB diagnosis in both veterinary and clinical settings.

show abstract

“…Both live and killed vaccines induce similar ex vivo IFN-γ ELISPOT responses, but vaccination with live BCG elicits stronger long-term cultured IFN-γ ELISPOT responses than do killed vaccine preparations. Interestingly, live vaccines are protective while killed formulations fail to provide protection against challenge with virulent tuberculous mycobacteria [31][32]33 . Assessment of Tcm responses is also feasible by sorting these cells from PBMC.…”

Section: Discussionmentioning

confidence: 99%

“…The long-term cultured IFN-γ ELISPOT response has been applied to assess memory responses by human, and cattle when evaluating tuberculosis vaccine responses [14][15][16][31][32]33 . Tcm responses are also assumed to play significant roles in host responses to several other infectious agents of cattle, such as: Mycoplasma mycoides subsp.…”

Section: Discussionmentioning

confidence: 99%

Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle

Maggioli

Palmer

Vordermeier³

et al. 2015

JoVE

View full text Add to dashboard Cite

Effector and memory T cells are generated through developmental programing of naïve cells following antigen recognition. If the infection is controlled up to 95 % of the T cells generated during the expansion phase are eliminated (i.e., contraction phase) and memory T cells remain, sometimes for a lifetime. In humans, two functionally distinct subsets of memory T cells have been described based on the expression of lymph node homing receptors. Central memory T cells express C-C chemokine receptor 7 and CD45RO and are mainly located in T-cell areas of secondary lymphoid organs. Effector memory T cells express CD45RO, lack CCR7 and display receptors associated with lymphocyte homing to peripheral or inflamed tissues. Effector T cells do not express either CCR7 or CD45RO but upon encounter with antigen produce effector cytokines, such as interferon-γ. Interferon-γ release assays are used for the diagnosis of bovine and human tuberculosis and detect primarily effector and effector memory T cell responses. Central memory T cell responses by CD4 + T cells to vaccination, on the other hand, may be used to predict vaccine efficacy, as demonstrated with simian immunodeficiency virus infection of non-human primates, tuberculosis in mice, and malaria in humans. Several studies with mice and humans as well as unpublished data on cattle, have demonstrated that interferon-γ ELISPOT assays measure central memory T cell responses. With this assay, peripheral blood mononuclear cells are cultured in decreasing concentration of antigen for 10 to 14 days (long-term culture), allowing effector responses to peak and wane; facilitating central memory T cells to differentiate and expand within the culture.

show abstract

Protection of cattle from bovine tuberculosis by vaccination with BCG by the respiratory or subcutaneous route, but not by vaccination with killed Mycobacterium vaccae

Cited by 137 publications

References 30 publications

Lymphocyte Recruitment and Protective Efficacy against Pulmonary Mycobacterial Infection Are Independent of the Route of PriorMycobacterium bovisBCG Immunization

Lymphocyte Recruitment and Protective Efficacy against Pulmonary Mycobacterial Infection Are Independent of the Route of PriorMycobacterium bovisBCG Immunization

Development of a Skin Test for Bovine Tuberculosis for Differentiating Infected from Vaccinated Animals

Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle

Contact Info

Product

Resources

About

Protection of cattle from bovine tuberculosis by vaccination with BCG by the respiratory or subcutaneous route, but not by vaccination with killed Mycobacterium vaccae

Cited by 137 publications

References 30 publications

Lymphocyte Recruitment and Protective Efficacy against Pulmonary Mycobacterial Infection Are Independent of the Route of PriorMycobacterium bovisBCG Immunization

Lymphocyte Recruitment and Protective Efficacy against Pulmonary Mycobacterial Infection Are Independent of the Route of PriorMycobacterium bovisBCG Immunization

Development of a Skin Test for Bovine Tuberculosis for Differentiating Infected from Vaccinated Animals

Application of Long-term cultured Interferon-&#947; Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle

Contact Info

Product

Resources

About

Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle