Protection against abortion linked to gamma interferon production in pregnant dairy cows naturally infected with Neospora caninum

López‐Gatius, F.; Almerı́a, S.; Donofrío, Gaetano; Nogareda, C.; García-Ispierto, Irina; Bech-Sàbat, G.; Santolaria, P.; Yániz, J.L.; Pabón, M.; Sousa, Noelita Melo de; Beckers, J. F.

doi:10.1016/j.theriogenology.2007.08.006

Cited by 43 publications

(37 citation statements)

References 36 publications

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“…The present study is the first report of the induction of potentially protective cell-mediated and humoral immune responses in cattle by using subunits of N. caninum antigens. The immune responses induced by NcSRS2-lipopeptides shown in the present study, namely, T-lymphocyte proliferation in and IFN-␥ secretion by PBMC, were similar to the immune responses induced by live N. caninum infection in previous studies (22,39,43). The induction of CD4 ϩ cytotoxic T-lymphocytes, T-lymphocyte proliferation in PBMC, and IFN-␥-secreting cells in PBMC are evident in cattle experimentally infected with N. caninum, including pregnant cattle (1,36,40).…”

Section: Discussionsupporting

confidence: 89%

“…We show here that palmitic acid-modified peptides of N. caninum tachyzoite surface antigen NcSRS2 containing T-lymphocytebearing epitopes (39) stimulated both T-lymphocyte and Blymphocyte immune responses in cattle following immunization. The T-lymphocyte activation and IFN-␥ secretion induced by NcSRS2-lipopeptide vaccination in this study mimic previously reported cell-mediated immune responses following experimental infection with live N. caninum parasites (18, 39, 43), as well as immune responses in field studies investigating their correlation with protection from N. caninuminduced abortion (22). The NcSRS2 DNA subunit immunization alone did not stimulate either T-lymphocyte or B-lymphocyte immune responses following DNA immunization, despite using GM-CSF and Flt3 ligand, adjuvants known to assist strong immune stimulation in cattle immunized for other pathogens (28,29).…”

Section: Discussionsupporting

confidence: 85%

“…The induction of CD4 ϩ cytotoxic T-lymphocytes, T-lymphocyte proliferation in PBMC, and IFN-␥-secreting cells in PBMC are evident in cattle experimentally infected with N. caninum, including pregnant cattle (1,36,40). More importantly, parasitespecific immune responses involving T-lymphocyte activation and IFN-␥ secretion in PBMC are associated with protection against N. caninum-induced fetal transmission and death (19,22,43). Because the induction of cell-mediated immune responses and IFN-␥ secretion appear crucial to fetal protection and NcSRS subunits induce those same immune responses, it is logical to extend the study toward testing the efficacy of NcSRS2-lipopeptide immunization against fetal infection and death in pregnant cattle.…”

Section: Discussionmentioning

confidence: 99%

“…Field observations suggest that natural immunity to N. caninum-induced abortion develops (22). Critical parameters to consider with vaccine development for bovine neosporosis were recently recommended by the World Association for the Advancement of Veterinary Parasitology (9).…”

Section: Discussionmentioning

confidence: 99%

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Bovine Immune Response to Inoculation with Neospora caninum Surface Antigen SRS2 Lipopeptides Mimics Immune Response to Infection with Live Parasites

Baszler

Shkap²,

Mwangi

et al. 2008

Clin Vaccine Immunol

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Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Bovine Immune Response to Inoculation with Neospora caninum Surface Antigen SRS2 Lipopeptides Mimics Immune Response to Infection with Live Parasites

Baszler

Shkap²,

Mwangi

et al. 2008

Clin Vaccine Immunol

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show abstract

“…These vaccination studies showed the importance of the adaptive immune response in defense against N. caninum in different species. A type 1 T-helper-cell (TH1) response was found to protect against natural (27) and experimental N. caninum infections (22). During pregnancy, a shift of the immune response toward a TH2 response was described for mice (24,25).…”

Section: Discussionmentioning

confidence: 99%

Indoleamine 2,3-Dioxygenase Is Involved in Defense againstNeospora caninumin Human and Bovine Cells

et al. 2009

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Neospora caninum is an apicomplexan parasite closely related to Toxoplasma gondii. In nature this parasite is found especially in dogs and cattle, but it may also infect other livestock. The growth of N. caninum, which is an obligate intracellular parasite, is controlled mainly by the cell-mediated immune response. During infection the cytokine gamma interferon (IFN-␥) plays a prominent role in regulating the growth of N. caninum in natural and experimental disease. The present study showed that induction of the tryptophan-degrading enzyme indoleamine 2,3-dioxygenase (IDO) is responsible for the inhibition of parasite growth that is mediated by IFN-␥-activated bovine fibroblasts and endothelial cells. This antiparasite effect could be abrogated by addition of tryptophan, as well as by the IDO-specific inhibitor 1-L-methyltryptophan. In conclusion, our data show that human and bovine cells use the same effector mechanism to control the growth of N. caninum.

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Placental colonization with periodontal pathogens: the potential missing link

Fischer

Demerath

Bittner-Eddy

et al. 2019

American Journal of Obstetrics and Gynecology

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Observational studies demonstrate that women with severe periodontitis have a higher risk of adverse pregnancy outcomes like preterm birth and low birthweight. Standard treatment for periodontitis in the form of scaling and root planing during the second trimester failed to reduce the risk of preterm or low birthweight. It is premature to dismiss the association between periodontitis and adverse pregnancy outcomes because one explanation for the failure of scaling and root planing to reduce the risk of adverse pregnancy outcomes is that periodontal pathogens spread to the placental tissue prior to periodontal treatment. In the placenta, orally derived organisms could cause direct tissue damage or mediate a maternal immune response that impairs the growth of the developing fetus. Sequencing studies demonstrate the presence of organisms derived from the oral microbiome in the placenta, but DNA-based sequencing studies should not be the only technique to evaluate the placental microbiome because they may not detect important shifts in the metabolic capability of the microbiome. In humans, polymerase chain reaction and histology have detected periodontal pathogens in placental tissue in association with multiple adverse pregnancy outcomes. We conclude that both placental and oral microbiomes may play a role in periodontitis-associated adverse pregnancy outcomes. However, the measure to determine the association between periodontal pathogens in the placenta and adverse pregnancy outcomes should be the amount and prevalence, not the mere presence of such microorganisms. Placental colonization with periodontal pathogens thus potentially represents the missing link between periodontitis and adverse pregnancy outcomes.

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Protection against abortion linked to gamma interferon production in pregnant dairy cows naturally infected with Neospora caninum

Cited by 43 publications

References 36 publications

Bovine Immune Response to Inoculation with Neospora caninum Surface Antigen SRS2 Lipopeptides Mimics Immune Response to Infection with Live Parasites

Bovine Immune Response to Inoculation with Neospora caninum Surface Antigen SRS2 Lipopeptides Mimics Immune Response to Infection with Live Parasites

Indoleamine 2,3-Dioxygenase Is Involved in Defense againstNeospora caninumin Human and Bovine Cells

Placental colonization with periodontal pathogens: the potential missing link

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