Protease‐Activatable Collagen Targeting Based on Protein Cyclization

Breurken, M Monica; Lempens, Ehm Edith; Merkx, Maarten

doi:10.1002/cbic.201000223

Cited by 8 publications

(10 citation statements)

References 30 publications

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“…Macromolecules sensitized to a user-defined signal represent important tools in chemical and cellular biology, with potential applications in medical diagnostics. A variety of engineering techniques have been developed to produce macromolecular switches, yet these efforts have focused primarily on modifying single-chain proteins (for recent examples see [1][2][3][4][5] ). We have designed an approach suited to controlling the growing number of "split" proteins, comprised of self-assembling protein fragments.…”

mentioning

confidence: 99%

Protease Activation of Split Green Fluorescent Protein

2010

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mentioning

confidence: 99%

Protease Activation of Split Green Fluorescent Protein

2010

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“…For example, protease‐activatable probes have been designed to result in specific retention of the imaging label by tagging of an MMP‐2/14 activated transmembrane domain to the cell membrane or specific internalization of an MMP‐9 activated probe into scavenger receptor class A type I expressing macrophages . Another example is a fluorescent probe that showed enhanced binding to collagen fibers upon proteolytic activation in vitro .…”

Section: Bioresponsive Probes Facilitating Site‐specific Retention Ofmentioning

confidence: 99%

Bioresponsive probes for molecular imaging: concepts and in vivo applications

Duijnhoven

Robillard

Langereis

et al. 2015

Contrast Media & Molecular

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Molecular imaging is a powerful tool to visualize and characterize biological processes at the cellular and molecular level in vivo. In most molecular imaging approaches, probes are used to bind to disease-specific biomarkers highlighting disease target sites. In recent years, a new subset of molecular imaging probes, known as bioresponsive molecular probes, has been developed. These probes generally benefit from signal enhancement at the site of interaction with its target. There are mainly two classes of bioresponsive imaging probes. The first class consists of probes that show direct activation of the imaging label (from "off" to "on" state) and have been applied in optical imaging and magnetic resonance imaging (MRI). The other class consists of probes that show specific retention of the imaging label at the site of target interaction and these probes have found application in all different imaging modalities, including photoacoustic imaging and nuclear imaging. In this review, we present a comprehensive overview of bioresponsive imaging probes in order to discuss the various molecular imaging strategies. The focus of the present article is the rationale behind the design of bioresponsive molecular imaging probes and their potential in vivo application for the detection of endogenous molecular targets in pathologies such as cancer and cardiovascular disease.

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“…Recombinant CNA35 constructs whose collagen-binding is activated by matrix metalloproteinases (MMPs) showed improved selectivity for collagens undergoing MMP-mediated remodeling. [19,20]. Library approaches have been used to identify monoclonal antibody [21,22] and peptides [23] that specifically bind to cryptic sites in collagen strands that become exposed after denaturation.…”

Section: Collagen-targeting Moleculesmentioning

confidence: 99%

Targeting and mimicking collagens via triple helical peptide assembly

Li¹,

Yu²

2013

Current Opinion in Chemical Biology

103

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As the major structural component of the extracellular matrix, collagen plays a crucial role in tissue development and regeneration. Since structural and metabolic abnormalities of collagen are associated with numerous debilitating diseases and pathologic conditions, the ability to target collagens of diseased tissues could lead to new diagnostics and therapeutics. Collagen is also a natural biomaterial widely used in drug delivery and tissue engineering, and construction of synthetic collagen-like materials is gaining interests in the biomaterials community. The unique triple helical structure of collagen has been explored for targeting collagen strands, and for engineering collagen-like functional assemblies and conjugates. This review focuses on the forefront of research activities in the use of the collagen mimetic peptide for both targeting and mimicking collagens via its triple helix mediated strand hybridization and higher order assembly.

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Protease‐Activatable Collagen Targeting Based on Protein Cyclization

Cited by 8 publications

References 30 publications

Protease Activation of Split Green Fluorescent Protein

Protease Activation of Split Green Fluorescent Protein

Bioresponsive probes for molecular imaging: concepts and in vivo applications

Targeting and mimicking collagens via triple helical peptide assembly

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