Prostaglandin-H synthase containing cell lines as tools for studying metabolism and toxicity of xenobiotics

Procainamide, a Drug Causing Lupus, Induces Prostaglandin H Synthase-2 and Formation of T Cell-Sensitizing Drug Metabolites in Mouse Macrophages

“…It is known that during prostaglandin biosynthesis the peroxidase activity of PGHS reduces the endoperoxide-hydroperoxide substrate PGG 2 with electrons derived from oxidation of cosubstrates. The latter may be exogenous ones, such as arylamines (20,32,33). The fact that PGHS, indeed, can N-oxidize PA was demonstrated by our finding that incubation of PA with ovine PGHS yielded HAPA and led to covalent binding.…”

Procainamide, a Drug Causing Lupus, Induces Prostaglandin H Synthase-2 and Formation of T Cell-Sensitizing Drug Metabolites in Mouse Macrophages

“…It is known that during prostaglandin biosynthesis the peroxidase activity of PGHS reduces the endoperoxide-hydroperoxide substrate PGG 2 with electrons derived from oxidation of cosubstrates. The latter may be exogenous ones, such as arylamines (20,32,33). The fact that PGHS, indeed, can N-oxidize PA was demonstrated by our finding that incubation of PA with ovine PGHS yielded HAPA and led to covalent binding.…”

Chemical Exposures and Parkinson's Disease in Residents of Three Negev Kibbutzim

“…Stimulation of prostaglandin-H synthase and monooxygenase activity [common toxic effects observed in experimental toxicology (Degen, 1993;Schilderman et al, 1993)] can result in a decreased dopamine level (according to the current understanding of dopamine metabolism).…”

“…Autoimmune processes (Revillard, 1991) 6. Stimulation of prostaglandin-H synthase and monooxygenase activity (Degen, 1993;Schilderman et al, 1993) 7. Depletion of glutathione (Tazhibaev and Tishchenko, 1992) 8.…”

Detection of PCB Adducts by the 32P-Postlabeling Technique

“…We used HRP as the oxidizing enzyme, but oxidases with an identical or similar mechanism are present in most mammalian tissues (56). For example, prostaglandin H synthase (PHS) bioactivates many aromatic amines and phenols via a one-electron oxidation (57). Therefore, it is conceivable that hydroquinone metabolites of PCBs are oxidized by PHS to generate semiquinones and perhaps quinones.…”

Procainamide, a Drug Causing Lupus, Induces Prostaglandin H Synthase-2 and Formation of T Cell-Sensitizing Drug Metabolites in Mouse Macrophages

“…It is known that during prostaglandin biosynthesis the peroxidase activity of PGHS reduces the endoperoxide-hydroperoxide substrate PGG 2 with electrons derived from oxidation of cosubstrates. The latter may be exogenous ones, such as arylamines (20,32,33). The fact that PGHS, indeed, can N-oxidize PA was demonstrated by our finding that incubation of PA with ovine PGHS yielded HAPA and led to covalent binding.…”

Chemical Exposures and Parkinson's Disease in Residents of Three Negev Kibbutzim

“…Stimulation of prostaglandin-H synthase and monooxygenase activity [common toxic effects observed in experimental toxicology (Degen, 1993;Schilderman et al, 1993)] can result in a decreased dopamine level (according to the current understanding of dopamine metabolism).…”

“…Autoimmune processes (Revillard, 1991) 6. Stimulation of prostaglandin-H synthase and monooxygenase activity (Degen, 1993;Schilderman et al, 1993) 7. Depletion of glutathione (Tazhibaev and Tishchenko, 1992) 8.…”

Detection of PCB Adducts by the 32P-Postlabeling Technique

“…We used HRP as the oxidizing enzyme, but oxidases with an identical or similar mechanism are present in most mammalian tissues (56). For example, prostaglandin H synthase (PHS) bioactivates many aromatic amines and phenols via a one-electron oxidation (57). Therefore, it is conceivable that hydroquinone metabolites of PCBs are oxidized by PHS to generate semiquinones and perhaps quinones.…”

Procainamide, a Drug Causing Lupus, Induces Prostaglandin H Synthase-2 and Formation of T Cell-Sensitizing Drug Metabolites in Mouse Macrophages

“…It is known that during prostaglandin biosynthesis the peroxidase activity of PGHS reduces the endoperoxide-hydroperoxide substrate PGG 2 with electrons derived from oxidation of cosubstrates. The latter may be exogenous ones, such as arylamines (20,32,33). The fact that PGHS, indeed, can N-oxidize PA was demonstrated by our finding that incubation of PA with ovine PGHS yielded HAPA and led to covalent binding.…”

Chemical Exposures and Parkinson's Disease in Residents of Three Negev Kibbutzim

“…Stimulation of prostaglandin-H synthase and monooxygenase activity [common toxic effects observed in experimental toxicology (Degen, 1993;Schilderman et al, 1993)] can result in a decreased dopamine level (according to the current understanding of dopamine metabolism).…”

“…Autoimmune processes (Revillard, 1991) 6. Stimulation of prostaglandin-H synthase and monooxygenase activity (Degen, 1993;Schilderman et al, 1993) 7. Depletion of glutathione (Tazhibaev and Tishchenko, 1992) 8.…”

Detection of PCB Adducts by the 32P-Postlabeling Technique

“…We used HRP as the oxidizing enzyme, but oxidases with an identical or similar mechanism are present in most mammalian tissues (56). For example, prostaglandin H synthase (PHS) bioactivates many aromatic amines and phenols via a one-electron oxidation (57). Therefore, it is conceivable that hydroquinone metabolites of PCBs are oxidized by PHS to generate semiquinones and perhaps quinones.…”