Prospects of siRNA applications in regenerative medicine

Mottaghitalab, Fatemeh; Rastegari, Ali; Farokhi, Mehdi; Dinarvand, Rassoul; Hosseinkhani, Hossein; Ou, Keng-Liang; Pack, Daniel W.; Mao, Chuanbin; Dinarvand, Meshkat; Fatahi, Yousef; Atyabi, Fatemeh

doi:10.1016/j.ijpharm.2017.03.092

Cited by 29 publications

(17 citation statements)

References 170 publications

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“…For this reason, it is of great significance to activate and promote the secretion of endogenous neurotrophic factors instead of using exogenous neurotrophic factors. With the development of life science, many genetic modification techniques have been applied to tissue engineering, but they often raise the risk of tumorigenesis (Lu et al, 2013;Fatemeh et al, 2017). However, ES, a relatively safe method, gradually attracts people's attention and is proved to promote the secretion of BDNF.…”

Section: Introductionmentioning

confidence: 99%

Conductive Hydroxyethyl Cellulose/Soy Protein Isolate/Polyaniline Conduits for Enhancing Peripheral Nerve Regeneration via Electrical Stimulation

Chen

Xiao

et al. 2020

Front. Bioeng. Biotechnol.

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Section: Introductionmentioning

confidence: 99%

Conductive Hydroxyethyl Cellulose/Soy Protein Isolate/Polyaniline Conduits for Enhancing Peripheral Nerve Regeneration via Electrical Stimulation

Chen

Xiao

et al. 2020

Front. Bioeng. Biotechnol.

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“…In this way, embryonic stem cell ESCs can also be used as vehicles for the delivery of agents to damaged tissue [76,77]. As an example, MSCs have been shown to efficiently and specifically carry drug-loaded polymeric nanoparticles by efflux transporters, such as overexpressed P-glycoprotein, since they are highly drug resistant [78]. Several studies successfully introduced siRNAs and other transgenes into MSCs while maintaining their proliferation ability and their capability to differentiate into different mesodermal lineages (bone, cartilage and fat) [78].…”

Section: Stem Cells and Their Clinical Potential For Retinal Diseasesmentioning

confidence: 99%

Nanocarriers, Progenitor Cells, Combinational Approaches, and New Insights on the Retinal Therapy

Pishavar

Luo

Bolander

et al. 2021

IJMS

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Progenitor cells derived from the retinal pigment epithelium (RPECs) have shown promise as therapeutic approaches to degenerative retinal disorders including diabetic retinopathy, age-related macular degeneration and Stargardt disease. However, the degeneration of Bruch’s membrane (BM), the natural substrate for the RPE, has been identified as one of the major limitations for utilizing RPECs. This degeneration leads to decreased support, survival and integration of the transplanted RPECs. It has been proposed that the generation of organized structures of nanofibers, in an attempt to mimic the natural retinal extracellular matrix (ECM) and its unique characteristics, could be utilized to overcome these limitations. Furthermore, nanoparticles could be incorporated to provide a platform for improved drug delivery and sustained release of molecules over several months to years. In addition, the incorporation of tissue-specific genes and stem cells into the nanostructures increased the stability and enhanced transfection efficiency of gene/drug to the posterior segment of the eye. This review discusses available drug delivery systems and combination therapies together with challenges associated with each approach. As the last step, we discuss the application of nanofibrous scaffolds for the implantation of RPE progenitor cells with the aim to enhance cell adhesion and support a functionally polarized RPE monolayer.

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“…Доказанное на экспериментальной модели ОРП свойство оРНК одновременно поддерживать в клетках печени процессы апоптоза и индуцировать в них митотическую активность свидетельствует о том, что оРНК способна переключать на ранней фазе регенерационного процесса активировавшийся апоптоз на пролиферацию клеток. Обнаруженный эффект с большой вероятностью обусловлен наличием регуляторных молекул РНК, в том числе многочисленных белок-некодирующих РНК [12][13][14], активно участвующих в регенерационных процессах и входящих в состав оРНК ККМ.…”

Section: результаты и их обсуждениеunclassified

Activation of regenerative processes in the liver when using cell-bone marrow total RNA

Онищенко

Фоменко

Никольская

et al. 2020

RJTAO

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Objective: to study the cellular mechanisms of activation of regenerative processes in the liver when using total RNA (tRNA) of bone marrow cells (BMCs) based on an extended liver resection (ELR) model. Materials and methods. Male Wistar rats (n = 80) with ELR model (70%) were divided into 2 groups: group 1 (control group) had a single saline injection, while group 2 (experimental group) received a single tRNA injection at a 30 μg/100 g dose of animal weight. The biochemical parameters of liver function and weight were monitored over time. Also monitored were microstructural changes in hepatocytes 48 hours after ELR by examining mitotic activity, caspase-9 expression and morphometric parameters. Results. It was found that in group 2, in comparison to group 1, there was faster normalization of biochemical parameters (by 10–14 days), a higher mitotic index of hepatocytes (23.45‰ versus 5.37‰), and initially sharper decrease and then faster recovery of liver mass (by 10–12 days versus 18–20 days). Both groups showed almost total expression of caspase-9, including in mitotically splitting hepatocytes. Group 1 demonstrated decreased values of morphometric parameters of single and binuclear cells, decreased number of binucleated hepatocytes and increased total density of hepatocytes as compared to the intact liver. Intraperitoneal administration of tRNA increased morphometric parameters of mononuclear hepatocytes, did not affect their number, but increased the area of the nuclei of binuclear hepatocytes as compared to the control group. Conclusion. The proven capability of cell-bone marrow total RNA to simultaneously support apoptosis in liver cells after ELR and induce mitotic activity indicates that tRNA can switch activated apoptosis to cell proliferation at the early phase of the regenerative process. This effect may be due to the presence of regulatory RNA molecules in tRNA, including numerous non-coding RNAs.

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Prospects of siRNA applications in regenerative medicine

Cited by 29 publications

References 170 publications

Conductive Hydroxyethyl Cellulose/Soy Protein Isolate/Polyaniline Conduits for Enhancing Peripheral Nerve Regeneration via Electrical Stimulation

Conductive Hydroxyethyl Cellulose/Soy Protein Isolate/Polyaniline Conduits for Enhancing Peripheral Nerve Regeneration via Electrical Stimulation

Nanocarriers, Progenitor Cells, Combinational Approaches, and New Insights on the Retinal Therapy

Activation of regenerative processes in the liver when using cell-bone marrow total RNA

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