2010
DOI: 10.1089/scd.2009.0445
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Abstract: A prospective in vivo assay was used to identify cells with potential for multiple lineage differentiation. With this assay it was first determined that the 5-FU resistant cells capable of osseous tissue formation in vivo also migrated towards SDF-1 in vitro. In parallel, an isolation method based upon fluorescence activated cell sorting (FACS) was employed to identify a very small cell embryonic-like (VSEL) Lin−Sca-1+ CD45− cell that with as few as 500 cells were capable of forming bone-like structures in viv… Show more

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Cited by 86 publications
(89 citation statements)
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“…Apart from umbilical cord blood and bone marrow, VSELs have also been reported in Wharton's jelly and gonadal tissue [46][47][48][49][50][51]. Their presence amongst the MSCs in the Wharton's jelly is in agreement with observations made by other groups that MSCs contain a sub-population of more primitive stem cells [52] or even as postulated by Taichman and group [53] that VSELs are precursors of MSCs. Various studies have also reported that VSELs are mobilized into peripheral blood in response to injury/ stress in animal models [27,[54][55][56] as well as in humans [28][29][30]57] -thus suggesting a role in regeneration and homeostasis.…”
Section: Developmental Origin Of Vselssupporting
confidence: 87%
“…Apart from umbilical cord blood and bone marrow, VSELs have also been reported in Wharton's jelly and gonadal tissue [46][47][48][49][50][51]. Their presence amongst the MSCs in the Wharton's jelly is in agreement with observations made by other groups that MSCs contain a sub-population of more primitive stem cells [52] or even as postulated by Taichman and group [53] that VSELs are precursors of MSCs. Various studies have also reported that VSELs are mobilized into peripheral blood in response to injury/ stress in animal models [27,[54][55][56] as well as in humans [28][29][30]57] -thus suggesting a role in regeneration and homeostasis.…”
Section: Developmental Origin Of Vselssupporting
confidence: 87%
“…While the case for pluripotency and quiescence of VSEL cells has not been convincingly made, murine VSEL cells have indeed been shown to have proliferation capacity and differentiation potential in vitro (2,13). Furthermore, murine VSEL cells are able to form bone tissue in vivo, and may contribute to heart regeneration (14,15), although the same cells failed in hematopoietic reconstitution assays and in teratoma formation assays (2,11). Most recently, a population similar to murine VSEL cells has been identified in rat bone marrow and has been shown both to expand and differentiate in vitro and to contribute to heart regeneration in vivo (16).…”
Section: Sca-1mentioning
confidence: 99%
“…Previously, we demonstrated that a significant proportion of the osseous regenerative capacity resides in a low-density cellular fraction, which is resistant to agents that induce apoptosis of cells actively undergoing DNA synthesis [4]. Furthermore, this population expresses the G-coupled receptor CXCR4 and therefore migrates rapidly in response to stromal-derived factor-1 (SDF-1 or CXCL12) [5]. Fluorescence activated cell sorting (FACS) further identified very small cells that do not express CD45 or other hematopoietic lineage markers (Lin -), and in mouse marrow expresses the Sca-1 antigen [6,7].…”
Section: Introductionmentioning
confidence: 99%
“…Therefore, the cells were described as very small embryonic-like (VSEL) cells [8,9]. Freshly isolated murine VSEL (MuVSEL) cells, when implanted in vivo, generated mineralized structures with as few as 500 cells, and when transplanted to a bone marrow environment were able to differentiate into adipocytes [5].…”
Section: Introductionmentioning
confidence: 99%