Prospective biomarkers of stem cells of human endometrium and fallopian tube compared with bone marrow

Abstract

The applicability of stem cells from the human endometrium and fallopian tube for regeneration is a fascinating area of research because of the role of these cells in dynamic tissue remodelling and their cyclical regenerative property during the menstrual cycle and pregnancy. Nevertheless, studies on the identity of biomarkers of these stem cells are limited and need to be extended. The present study has aimed at exploring the tissue-specific biomarkers of stem cells derived from the human endometrium and fallopian tube compared with those from bone marrow. Cells were isolated from human endometrium and fallopian tubes and characterized for biomarkers, including CD34, CD133, CD117, CD90, CD105, CD73, nestin, CD29, CD44, CD31, CD54, CD166, CD106, CD49d, CD45, ABCG2, SSEA4, OCT4, SOX2, CD140b and CD146, by flowcytometry. Both endometrium and fallopian tube sources exhibited positivity over a wide range of markers, as did bone marrow. In particular, they exhibited pluripotency, perivascular and mesenchymal stem cell markers and cell adhesion molecules, thereby suggesting their relevance in tissue repair and regeneration. Overall, the results of this study provide evidence for the presence of stem cells in the human endometrium and fallopian tube, which could thus represent additional stem cell sources for regenerative medicine.

Isolation and characterization of equine endometrial mesenchymal stromal cells

“…A limited number of studies have compared the features of endometrial MSCs with MSCs from other sources [23, 44]. Our finding based on results of flow cytometry and qPCR, showing that endometrial MSCs in culture display moderately higher levels of CD29, CD90, and CD105 but lower levels of NG2 than their BM counterparts, is consistent with data from Indumathi et al [23].…”

“…A limited number of studies have compared the features of endometrial MSCs with MSCs from other sources [23, 44]. Our finding based on results of flow cytometry and qPCR, showing that endometrial MSCs in culture display moderately higher levels of CD29, CD90, and CD105 but lower levels of NG2 than their BM counterparts, is consistent with data from Indumathi et al [23]. Whether this is indicative of differences in the abundance of stem cells between the two tissue sources or reflects tissue-specific changes in immunophenotype that may be induced in culture should be investigated in future studies.…”

Marker Profiles of Human Endometrial Stem Cells at Various Passages Cultured In-Vitro

“…Thus, identifying the tissue-specific stem cell markers in normal endometrium paves way to identify the same in disease condition. Our study carried out a marker profiling of endometrial cells in heterogenous fresh isolates [7]. However, as reported in several literatures, evaluating homogenous MSCs is equally important for ascertaining its utility in wound healing and repair mechanisms.…”

“…The samples were isolated according to Indumathi et al [7]. The samples were collected and transported to laboratory by aseptic technique.…”

“…Although several markers have been identified in stem cells of endometrium, including our previously published paper [6,7], there are no current literatures to check its marker characterization at various passages cultured in-vitro. It is understood from the literatures that mesenchymal stem cells are potent source of wound healing and repair mechanisms [8,9].…”