Prophylactic and immune modulatory influences of Nigella sativa Linn. in broilers exposed to biological challenge

Abstract

Background and Aim:Prophylaxis and disease prevention is an essential strategy among biorisk management in poultry farms that stimulate and maintain the birds’ immunity. The aim of this study was to investigate the prophylactic, and immune-stimulant influence of Nigella sativa Linn. in broilers under biological stress.Materials and Methods:A total of 250 1-day-old (ross) chicks were divided into 5 groups; four of which were supplemented with 1.4%, 2.8%, 4.2%, and 5.6% N. sativa Linn., respectively. The four supplemented groups were challenged with Escherichia coli O157:H7 1.5×108 at a 14th day old. A total of 1050 samples (150 serum, 150 swab, and 750 organ samples) were collected and examined.Results:A highly significant increase (p<0.01) in 5.6% N. sativa Linn. supplemented group in performance traits (body weight, weight gain, and performance index), biochemical parameters (proteinogram, liver enzymes, and creatinine), immunoglobulins concentration, and immune organs’ weight. Meanwile, liver showed improvement of histoarchitecture without fibrosis. Heart showed a mild pericarditis with a mild degree of hydropic degeneration. Bursa, thymus, and spleen showed lymphoid hyperplasia.Conclusion:A concentration of 5.6% N. sativa Linn. in broiler’s feed can improve the immune response and subsequent resistance of broilers against diseases.

Influence of <i>Nigella sativa</i> and rosemary oils on growth performance, biochemical, antioxidant and immunological parameters, and pathological changes in Japanese quail challenged with <i>Escherichia coli</i>

“…The analysis of serum biochemical parameters gives an indication about the health, nutritional and physiological status of animals (Abd El-Hack and . In this study, the results of serum of liver and kidney function biomarkers, and TP and Glob levels were in agreement with the results of Khan et al (2012), Soliman et al (2017) and El-Hack et al (2018). The increase in serum TP may be referred to the immunostimulant effect of N. sativa (Al-Beitawi and El-Ghousein, 2008) and the availability of black cumin seed proteins in birds diets (Khan et al, 2012).…”

“…Additionally, RO significantly increased phagocytic activity and phagocytic index in broilers (Abd El-Latif et al, 2013). Moreover, black cumin may have an immunostimulant effect by increasing antibody titer against Newcastle disease (ND) and infectious Bursal disease as well as IgG and IgA contents in broilers challenged with E. coli and fed 4.2 or 5.6% N. sativa (Soliman et al, 2017).…”

Effect of black seed on immunity in young healthy Saudi volunteers

“…The study revealed highly significant increase (p<0.01) in IgG and IgA in NS 4.2% and NS 5.6% fed groups, on the other hand, IgM showed non-significant difference in all groups. A highly significant (p<0.01) decline in total bacterial count and total Enterobacteriaceae count was also noticed in the NS 5.6% fed broilers (21) .…”

Impact of lighting color and duration on productive performance and Newcastle disease vaccination efficiency in broiler chickens

“…Blood samples were collected, held at 37°C for 2 h, and centrifuged at 3500 rpm for 15 min. Clear sera were dispensed into Eppendorf tubes, tested for blood sugars, and stored at −20°C for biochemical, hormonal, and immunological analysis [18]. Birds were slaughtered after blood sampling; carcasses were weighed (total CW and expressed by CW/g), liver, heart, spleen, and bursa were removed, weighed, and expressed as g/kg.…”