Properties of the Escherichia coli RuvA and RuvB proteins involved in DNA repair, recombination and mutagenesis

Escherichia coli RuvA and RuvB proteins specifically interact with Holliday junctions and promote branch migration.

“…To explain these phenomena, we proposed that UV adducts induce cruciform structures in the supercoiled DNA, with which RuvA interacts to enhance the ATPase activity of RuvB . From these observations, we also proposed that the RuvA-RuvB complex specifically interacts with the Holliday junction and promotes branch migration Shiba et al 1991;Shinagawa et al 1991). Consistent with this hypothesis, RuvA binds to synthetic Holliday junctions with much higher affinity than to linear duplex DNA.…”

Escherichia coli RuvA and RuvB proteins specifically interact with Holliday junctions and promote branch migration.

“…To explain these phenomena, we proposed that UV adducts induce cruciform structures in the supercoiled DNA, with which RuvA interacts to enhance the ATPase activity of RuvB . From these observations, we also proposed that the RuvA-RuvB complex specifically interacts with the Holliday junction and promotes branch migration Shiba et al 1991;Shinagawa et al 1991). Consistent with this hypothesis, RuvA binds to synthetic Holliday junctions with much higher affinity than to linear duplex DNA.…”

Suramin action in African trypanosomes involves a RuvB-like DNA helicase

“…It forms a hexameric ring around the DNA and functions as an ATP-driven motor that promotes heteroduplex formation ( Wyatt and West, 2014 ). RuvB-like DNA helicases have been well studied in bacteria ( Iwasaki et al, 1992 ; Shinagawa et al, 1991 ; Yamada et al, 2001 ). In eukaryotes, the proteins RuvB-like 1 and RuvB-like 2 are also called pontin and reptin ( Jha and Dutta, 2009 ), respectively, and they have acquired additional functions including DNA repair and chromatin remodelling ( Dauden et al, 2021 ; Tammana and Tammana, 2017 ).…”

Suramin action in African trypanosomes involves a RuvB-like DNA helicase

“…It forms a hexameric ring around the DNA and functions as an ATP-driven motor that promotes heteroduplex formation (Wyatt, 2014). RuvB-like DNA helicases have been well studied in bacteria (Shinagawa, 1991; Iwasaki, 1992; Yamada, 2001). In eukaryotes, the proteins RuvB-like 1 and RuvB-like 2 are also called pontin and reptin (Jha, 2009), respectively, and they have acquired additional functions including DNA repair and chromatin remodeling (Tammana, 2017; Dauden, 2021).…”

Escherichia coli RuvA and RuvB proteins specifically interact with Holliday junctions and promote branch migration.

“…To explain these phenomena, we proposed that UV adducts induce cruciform structures in the supercoiled DNA, with which RuvA interacts to enhance the ATPase activity of RuvB . From these observations, we also proposed that the RuvA-RuvB complex specifically interacts with the Holliday junction and promotes branch migration Shiba et al 1991;Shinagawa et al 1991). Consistent with this hypothesis, RuvA binds to synthetic Holliday junctions with much higher affinity than to linear duplex DNA.…”

Suramin action in African trypanosomes involves a RuvB-like DNA helicase

“…It forms a hexameric ring around the DNA and functions as an ATP-driven motor that promotes heteroduplex formation ( Wyatt and West, 2014 ). RuvB-like DNA helicases have been well studied in bacteria ( Iwasaki et al, 1992 ; Shinagawa et al, 1991 ; Yamada et al, 2001 ). In eukaryotes, the proteins RuvB-like 1 and RuvB-like 2 are also called pontin and reptin ( Jha and Dutta, 2009 ), respectively, and they have acquired additional functions including DNA repair and chromatin remodelling ( Dauden et al, 2021 ; Tammana and Tammana, 2017 ).…”

Suramin action in African trypanosomes involves a RuvB-like DNA helicase

“…It forms a hexameric ring around the DNA and functions as an ATP-driven motor that promotes heteroduplex formation (Wyatt, 2014). RuvB-like DNA helicases have been well studied in bacteria (Shinagawa, 1991; Iwasaki, 1992; Yamada, 2001). In eukaryotes, the proteins RuvB-like 1 and RuvB-like 2 are also called pontin and reptin (Jha, 2009), respectively, and they have acquired additional functions including DNA repair and chromatin remodeling (Tammana, 2017; Dauden, 2021).…”

Escherichia coli RuvA and RuvB proteins specifically interact with Holliday junctions and promote branch migration.

“…To explain these phenomena, we proposed that UV adducts induce cruciform structures in the supercoiled DNA, with which RuvA interacts to enhance the ATPase activity of RuvB . From these observations, we also proposed that the RuvA-RuvB complex specifically interacts with the Holliday junction and promotes branch migration Shiba et al 1991;Shinagawa et al 1991). Consistent with this hypothesis, RuvA binds to synthetic Holliday junctions with much higher affinity than to linear duplex DNA.…”

Suramin action in African trypanosomes involves a RuvB-like DNA helicase

“…It forms a hexameric ring around the DNA and functions as an ATP-driven motor that promotes heteroduplex formation ( Wyatt and West, 2014 ). RuvB-like DNA helicases have been well studied in bacteria ( Iwasaki et al, 1992 ; Shinagawa et al, 1991 ; Yamada et al, 2001 ). In eukaryotes, the proteins RuvB-like 1 and RuvB-like 2 are also called pontin and reptin ( Jha and Dutta, 2009 ), respectively, and they have acquired additional functions including DNA repair and chromatin remodelling ( Dauden et al, 2021 ; Tammana and Tammana, 2017 ).…”

Suramin action in African trypanosomes involves a RuvB-like DNA helicase

“…It forms a hexameric ring around the DNA and functions as an ATP-driven motor that promotes heteroduplex formation (Wyatt, 2014). RuvB-like DNA helicases have been well studied in bacteria (Shinagawa, 1991; Iwasaki, 1992; Yamada, 2001). In eukaryotes, the proteins RuvB-like 1 and RuvB-like 2 are also called pontin and reptin (Jha, 2009), respectively, and they have acquired additional functions including DNA repair and chromatin remodeling (Tammana, 2017; Dauden, 2021).…”