AGRADECIMENTOSAgradeço ao Instituto de Pesquisas Tecnológicas pela oportunidade de trabalhar e desenvolver este estudo nas dependências de seus laboratórios.Ao CNPq, CAPES e RUSP pelo apoio financeiro.À FOUSP pela oportunidade de cursar a pós-graduação.A todos os docentes da Disciplina de Clínica Integrada que me acolheram quando entrei para cursar o doutorado. À Profa. Dra. Maria Aparecida Borsatti pela sua dedicação, paciência e confiança.Ao Prof. Dr. Norberto Nobuo Sugaya por continuar sendo meu eterno orientador. À Profa. Dra. Maria Helena Ambrosio Zanin que, mesmo não sendo minha orientadora oficial, dedicou grande parte do seu tempo e conhecimento no desenvolvimento deste trabalho, muitas vezes me ensinando conhecimento básico sobre química.Ao Prof. Dr. Adriano Marim de Oliveira por sua paciência, dedicação e ensinamentos.À Dra. Natalia Neto Pereira Cerize por seus ensinamentos, paciência e dedicação.Ao Dr. João Guilherme Rocha Poço que, com sua busca em desenvolver produtos odontológicos anti-tártaro, me levou para a pesquisa no laboratório do IPT. Aos meus colegas de pós-graduação pela amizade e convivência.As bibliotecárias da FOUSP por toda paciência e dedicação na revisão bibliográfica. The aim of this study was to prepare electrospun nanofiber films cetylpyridinium chloride (CPC) incorporated test their potential use as antifungal therapy against Candida albicans. CPC was incorporated to three different polymeric solutions, one containing polyvinyl alcohol (PVA), the second containing polyvinylpyrrolidone (PVP) and the third prepared with PVP and poly (methyl methacrylate-co-ethyl acrylate-comethacrylate ammonium) (PMMA). These polymeric solutions were evaluated as to conductivity, viscosity and surface tension, then subjected to a electrospinning process to obtain nanofiber films. The morphology and structure of nanofiber films were analyzed using scanning electron microscopy (SEM). The characterization of nanofiber films was performed by thermogravimetric analysis (TG) and differential scanning calorimetry (DSC). The encapsulation efficiency of the CPC nanofibers and the release kinetic profile of CPC and porcine mucosa permeation (Franz cells) were assessed by spectrophotometry. It was also assessed the concentration of CPC to be incorporated (from 0.05 to 5%) in nanofiber to be minimal fungicidal fraction released against strains of C. albicans by disk diffusion tests, compared to 5% miconazole (MCZ). Under these conditions, the PVA solution showed the highest conductivity, viscosity and surface tension. CPC increased conductivity. Both CPC and PMMA did not alter the surface tension of PVP. PMMA reduced viscosity. The solution of PVP/PMMA formed a more uniform film with less beads. Thermograms (TGA / DSC) suggested that the electrospinning process changes the crystallinity of CPC. The encapsulation efficiency reaches 99% of CPC. The release rate of encapsulated CPC was slow during the experimental period of 24 hours. In a nanofibrilic film, the fungicidal activity of CPC 2.5% and 5% MCZ was si...