Properties of a Novel PBP2A Protein Homolog from Staphylococcus aureus Strain LGA251 and Its Contribution to the β-Lactam-resistant Phenotype

Kim, Choon; Milheiriço, Catarina; Gardete, Susana; Holmes, Mark A.; Holden, Matthew T. G.; Lencastre, Hermı́nia de; Tomasz, Alexander

doi:10.1074/jbc.m112.395962

Cited by 116 publications

(103 citation statements)

References 36 publications

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“…to all β-lactam, associated to resistance to cefoxitin. According to Kim et al (2012) and Cartwright et al (2013) resistance to oxacillin and cefoxitin shows phenotypically the presence of mecA gene. On the other hand resistance to cefoxitin with sensitivity to oxacillin, shows the presence of mecC gene, which is responsible for the production of a protein that links additional penicillin (PBP2a) that provides low affinity of linking to β-lactam drugs.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance in Bacterial Pathogens of Canine Otitis

Sfaciotte¹,

Bordin²,

Vignoto³

et al. 2015

American Journal of Animal and Veterinary Sciences

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Abstract:Otitis is one of the most frequent infections in dogs. This is attributed to the misuse of drugs enabling generation of multi-resistant micro-organisms. The emergence of multiresistant bacterial strains in veterinary medicine is a reality that must be studied and evaluated by the professionals. The objective of this study was to isolate and evaluate the antimicrobial susceptibility of bacterial pathogens of otitis in dogs. Otologic swabs were collected from 36 dogs with clinical otitis. 41 bacterial strains were isolated and antimicrobial susceptibility tests were performed by disk diffusion method with 34 antimicrobial agents. Presence of the resistance gene mecA of Staphylococcus was examined for 22 strains of staphylococci by PCR. A total of 1108 ratings antimicrobial agents were performed. The percentage of drug resistance was 34.66% (n = 384) of the assessments with partial or total resistance. Major bacterial pathogens were Staphylococcus spp. (65.85%), Pseudomonas spp. (12.19%) and Enterobacteria species (19.51%). 53.66% of the isolates were considered multiresistant. Antimicrobial agents considered most resistant in the strains studied were penicillin (75.00%), tetracyclin (50.00%), amoxicillin (48.78%), trimethoprimsulfamethoxazole (46.15%), clindamycin and rifampicin (43.24%). 11 strains were phenotypically characterized as MRS, 4 genotypically as MRS, 2 as MLSB-MRS and 2 as gram negative ESBL-producing.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance in Bacterial Pathogens of Canine Otitis

Sfaciotte¹,

Bordin²,

Vignoto³

et al. 2015

American Journal of Animal and Veterinary Sciences

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“…mecA is controlled by regulatory genes mecI and mecR1. MecI often combines with the mecA promoter and plays an inhibitor role (49). In the existence of β-lactam antibiotics, MecR1 promotes a signaling transduction pathway that causes activation of mecA (50).…”

Section: Avoid Antibiotics Targeting I: Meca Mutationmentioning

confidence: 99%

Nosocomial infection and its molecular mechanisms of antibiotic resistance

Xia

Gao

Tang

2016

BST

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“…Furthermore, the activity of the mecC product, namely, PBP2c (15), appears to be thermosensitive, with a decline at 37°C (16). This raises the question as to whether mecC-MRSA infections should be considered like mecA-MRSA infections and treated with vancomycin (17) or like MSSA infections, generally treated with ␤-lactams, such as flucloxacillin (18).…”

mentioning

confidence: 98%