Propagation of fluorescent light

Quantitative Fluorescence Sensing Through Highly Autofluorescent, Scattering, and Absorbing Media Using Mobile Microscopy

“…Among others, collagen, tryptophan, melanin, and nicotinamide adenine dinucleotide create a strong autofluorescence background with a broad emission bandwidth in the visible spectrum, which makes it quite difficult to detect fluorescent biomarkers or reactants through human skin. − Various methods have been implemented to investigate this challenging problem using different spectroscopy and sensing systems . Some of these include ratiometric techniques that take into account the measured fluorescence emission in addition to the reflected excitation light, techniques that can selectively record photons that propagate a short distance in tissue, , among other theoretical and numerical methods. − Different than these previous approaches, in this manuscript we focus on quantitative fluorescence sensing through highly autofluorescent, scattering, and absorbing media using an imaging system, i . e ., a wearable microscope.…”