Proneural gene-linked neurogenesis in zebrafish cerebellum

Kani, Shuichi; Bae, Young Ki; Shimizu, Takashi; Tanabe, Koji; Satou, Chie; Parsons, Michael; Scott, Ethan K.; Higashijima, Shin‐ichi; Hibi, Masahiko

doi:10.1016/j.ydbio.2010.03.024

Cited by 139 publications

(357 citation statements)

References 76 publications

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“…We sought to determine which cell types respond to injury and contribute to cellular regeneration. The transgenes ptf1a:DsRed and nestin:gfp distinctly label VZ progenitor cells and neuroepithelial-like progenitor cells, respectively, in the zebrafish cerebellum, and they can be used to monitor stem and progenitor cell activity (Kani et al, 2010;Kaslin et al, 2013). Furthermore, Ptf1a is required for the generation of all inhibitory neuronal lineages in the cerebellum (Hoshino et al, 2005).…”

Section: Cellular Recovery After Cerebellar Injurymentioning

confidence: 99%

Distinct roles of neuroepithelial-like and radial glia-like progenitor cells in cerebellar regeneration

et al. 2017

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Zebrafish can regenerate after brain injury, and the regenerative process is driven by resident stem cells. Stem cells are heterogeneous in the vertebrate brain, but the significance of having heterogeneous stem cells in regeneration is not understood. Limited availability of specific stem cells might impair the regeneration of particular cell lineages. We studied regeneration of the adult zebrafish cerebellum, which contains two major stem and progenitor cell types: ventricular zone and neuroepithelial cells. Using conditional lineage tracing we demonstrate that cerebellar regeneration depends on the availability of specific stem cells. Radial glia-like cells are thought to be the predominant stem cell type in homeostasis and after injury. However, we find that radial glia-like cells play a minor role in adult cerebellar neurogenesis and in recovery after injury. Instead, we find that neuroepithelial cells are the predominant stem cell type supporting cerebellar regeneration after injury. Zebrafish are able to regenerate many, but not all, cell types in the cerebellum, which emphasizes the need to understand the contribution of different adult neural stem and progenitor cell subtypes in the vertebrate central nervous system.

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Section: Cellular Recovery After Cerebellar Injurymentioning

confidence: 99%

Distinct roles of neuroepithelial-like and radial glia-like progenitor cells in cerebellar regeneration

et al. 2017

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“…Although the presence of an EGL in anamniotes has been debated in recent years (Wullimann et al, 2011), there is little evidence for its manifestation in the cerebellum of sharks (Rodriguez-Moldes et al, 2008;Chaplin et al, 2010), basal ray-finned fish (Butts et al, 2014) and early or adult zebrafish (Kaslin et al, 2009(Kaslin et al, , 2013Chaplin et al, 2010;Kani et al, 2010). We conclude that a transit amplifying precursor layer is also absent in the frog.…”

Section: Introductionmentioning

confidence: 58%

Transit amplification in the amniote cerebellum evolved via a heterochronic shift in NeuroD1 expression

2014

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The cerebellum has evolved elaborate foliation in the amniote lineage as a consequence of extensive Atoh1-mediated transit amplification in an external germinal layer (EGL) comprising granule cell precursors. To explore the evolutionary origin of this layer, we have examined the molecular geography of cerebellar development throughout the life cycle of Xenopus laevis. At metamorphic stages Xenopus displays a superficial granule cell layer that is not proliferative and expresses both Atoh1 and NeuroD1, a marker of postmitotic cerebellar granule cells. Premature misexpression of NeuroD1 in chick partially recapitulates the amphibian condition by suppressing transit amplification. However, unlike in the amphibian, granule cells fail to enter the EGL. Furthermore, misexpression of NeuroD1 once the EGL is established both triggers radial migration and downregulates Atoh1. These results show that the evolution of transit amplification in the EGL required adaptation of NeuroD1, both in the timing of its expression and in its regulatory function, with respect to Atoh1.

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“…Microinjection of Tol2-based plasmid DNA was performed as described previously (Kawakami, 2004;Urasaki et al, 2006 (Miyasaka et al, 2009;Kani et al, 2010;Kinkhabwala et al, 2011;Koyama et al, 2011) Stochastic labeling of V0 neurons with GFP. For stochastic expression of GFP in V0 neurons, gad1b:lDl-GFP, or huC:lDl-GFP was injected into one to four cell stage embryos of Tg[dbx1b:Cre] stable transgenic fish.…”

Section: Methodsmentioning

confidence: 99%

Generation of Multiple Classes of V0 Neurons in Zebrafish Spinal Cord: Progenitor Heterogeneity and Temporal Control of Neuronal Diversity

Satou

Kimura²,

Higashijima³

2012

J. Neurosci.

143

207

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The developing spinal cord is subdivided into distinct progenitor domains, each of which gives rise to different types of neurons. However, the developmental mechanisms responsible for generating neuronal diversity within a domain are not well understood. Here, we have studied zebrafish V0 neurons, those that derive from the p0 progenitor domain, to address this question. We find that all V0 neurons have commissural axons, but they can be divided into excitatory and inhibitory classes. V0 excitatory neurons (V0-e) can be further categorized into three groups based on their axonal trajectories; V0-eA (ascending), V0-eB (bifurcating), and V0-eD (descending) neurons. By using time-lapse imaging of p0 progenitors and their progeny, we show that inhibitory and excitatory neurons are produced from different progenitors. We also demonstrate that V0-eA neurons are produced from distinct progenitors, while V0-eB and V0-eD neurons are produced from common progenitors. We then use birth-date analysis to reveal that V0-eA, V0-eB, and V0-eD neurons arise in this order. By perturbing Notch signaling and accelerating neuronal differentiation, we predictably alter the generation of early born V0-e neurons at the expense of later born ones. These results suggest that multiple types of V0 neurons are produced by two distinct mechanisms; from heterogeneous p0 progenitors and from the same p0 progenitor, but in a time-dependent manner.

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Proneural gene-linked neurogenesis in zebrafish cerebellum

Cited by 139 publications

References 76 publications

Distinct roles of neuroepithelial-like and radial glia-like progenitor cells in cerebellar regeneration

Distinct roles of neuroepithelial-like and radial glia-like progenitor cells in cerebellar regeneration

Transit amplification in the amniote cerebellum evolved via a heterochronic shift in NeuroD1 expression

Generation of Multiple Classes of V0 Neurons in Zebrafish Spinal Cord: Progenitor Heterogeneity and Temporal Control of Neuronal Diversity

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