Promotion of wound healing using adipose-derived stem cells in radiation ulcer of a rat model

Abstract

BackgroundWound healing is a complex biologic process that involves the integration of inflammation, mitosis, angiogenesis, synthesis, and remodeling of the extracellular matrix. However, some wounds fail to heal properly and become chronic. Although some simulated chronic wound models have been established, an efficient approach to treat chronic wounds in animal models has not been determined. The aim of this study was to develop a modified rat model simulating the chronic wounds caused by clinical radiation ulcers and examine the treatment of chronic wounds with adipose-derived stem cells.ResultsSprague–Dawley rats were irradiated with an electron beam, and wounds were created. The rats received treatment with adipose-derived stem cells (ASCs), and a wound-healing assay was performed. The wound sizes after ASC treatment for 3 weeks were significantly smaller compared with the control condition (p < 0.01). Histological observations of the wound edge and immunoblot analysis of the re-epithelialization region both indicated that the treatment with ASCs was associated with the development of new blood vessels. Cell-tracking experiments showed that ASCs were colocalized with endothelial cell markers in ulcerated tissues.ConclusionsWe established a modified rat model of radiation-induced wounds and demonstrated that ASCs accelerate wound-healing.

Isolation, Culturing, Characterization and Aging of Adipose Tissue-derived Mesenchymal Stem Cells: A Brief Overview

“…Adipose tissue is obtained from different organs such as abdominal subcutaneous, inguinal, kidney, or epididymis under sterile conditions (Banas et al 2007;Zaminy et al 2008;Park et al 2010;Ren et al 2012;Ahmad and Shakori 2013;Huangm et al 2013). Adipose tissues are transported to the laboratory in phosphate-buffered saline (PBS) solution or Dulbecco's Modified Eagle's Medium (DMEM) containing antibiotics within 2 h postsurgery for further procedures (Banas et al 2007;Ahmad and Shakori 2013).…”

Adipose derived stem cells (ASC) in wound healing: Recent results in vitro and in vivo

“…Additionally, a reduction of stem cell marker-positive cells was shown. The release of the treatment with ASCs initiated smaller wound sizes and was associated with the development of new blood vessels 20 .…”

Substance P enhances mesenchymal stem cells-mediated immune modulation

“…Of the numerous functions of MSCs, their role in immune modulation has been considered as an important aspect in the development of MSC-based therapeutics. This ability for immune modulation renders MSC transplantation an efficient treatment for severe inflammatory diseases, including rheumatoid arthritis, skin wounds, and liver diseases [10][11][12]. Clinically, steroids are commonly used to block inflammatory responses; however, their use has been heavily monitored because of the resulting side effects, including osteoporosis and hyperglycemia [13].…”