Promoters of the Barley Germin-Like<i>GER4</i>Gene Cluster Enable Strong Transgene Expression in Response to Pathogen Attack

Himmelbach, Axel; Liu, Luo; Zierold, Uwe; Altschmied, Lothar; Maucher, Helmut; Beier, Franziska; Müller, Doreen; Hensel, Goetz; Heise, Andreas; Schützendübel, Andres; Kumlehn, Jochen; Schweizer, Patrick

doi:10.1105/tpc.109.067934

Cited by 84 publications

(67 citation statements)

References 77 publications

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“…RNA extraction, first-strand cDNA synthesis, and RT-PCR were performed as described previously (51). The young leaves of seedlings at three-leaf stage or BaMMV-inoculated leaves 5 wk postinoculation were sampled for total RNA extraction.…”

Section: Methodsmentioning

confidence: 99%

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PROTEIN DISULFIDE ISOMERASE LIKE 5-1is a susceptibility factor to plant viruses

Yang

Lüpken

Habekuß

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Protein disulfide isomerases (PDIs) catalyze the correct folding of proteins and prevent the aggregation of unfolded or partially folded precursors. Whereas suppression of members of the PDI gene family can delay replication of several human and animal viruses (e.g., HIV), their role in interactions with plant viruses is largely unknown. Here, using a positional cloning strategy we identified variants of PROTEIN DISULFIDE ISOMERASE LIKE 5-1 (HvPDIL5-1) as the cause of naturally occurring resistance to multiple strains of Bymoviruses. The role of wild-type HvPDIL5-1 in conferring susceptibility was confirmed by targeting induced local lesions in genomes for induced mutant alleles, transgene-induced complementation, and allelism tests using different natural resistance alleles. The geographical distribution of natural genetic variants of HvPDIL5-1 revealed the origin of resistance conferring alleles in domesticated barley in Eastern Asia. Higher sequence diversity was correlated with areas with increased pathogen diversity suggesting adaptive selection for bymovirus resistance. HvPDIL5-1 homologs are highly conserved across species of the plant and animal kingdoms implying that orthologs of HvPDIL5-1 or other closely related members of the PDI gene family may be potential susceptibility factors to viruses in other eukaryotic species.allele mining | resistance breeding | soil-borne virus disease | chaperone

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Section: Methodsmentioning

confidence: 99%

“…The barley UBIQUITIN CARRIER gene (NCBI accession no. AK361071, HvUBC) was used as endogenous constitutively expressed control (51).…”

Section: Methodsmentioning

confidence: 99%

PROTEIN DISULFIDE ISOMERASE LIKE 5-1is a susceptibility factor to plant viruses

Yang

Lüpken

Habekuß

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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“…Many of the root-specific transcripts represented secondary metabolic functions, predominantly monolignol and monoterpene biosynthesis. The roots also expressed six transcripts encoding germin-like proteins, which may help to form a defensive barrier during emergence from the soil but are also implicated in symbiosis (Himmelbach et al, 2010). Only a few tendril-specific transcripts were identified, including several related to auxin signaling/responses and one encoding a TT16-like MADS transcription factor that is thought to control organ growth in Arabidopsis (Prasad et al, 2010).…”

Section: Online)mentioning

confidence: 99%

The Grapevine Expression Atlas Reveals a Deep Transcriptome Shift Driving the Entire Plant into a Maturation Program

et al. 2012

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We developed a genome-wide transcriptomic atlas of grapevine (Vitis vinifera) based on 54 samples representing green and woody tissues and organs at different developmental stages as well as specialized tissues such as pollen and senescent leaves. Together, these samples expressed ;91% of the predicted grapevine genes. Pollen and senescent leaves had unique transcriptomes reflecting their specialized functions and physiological status. However, microarray and RNA-seq analysis grouped all the other samples into two major classes based on maturity rather than organ identity, namely, the vegetative/ green and mature/woody categories. This division represents a fundamental transcriptomic reprogramming during the maturation process and was highlighted by three statistical approaches identifying the transcriptional relationships among samples (correlation analysis), putative biomarkers (O2PLS-DA approach), and sets of strongly and consistently expressed genes that define groups (topics) of similar samples (biclustering analysis). Gene coexpression analysis indicated that the mature/woody developmental program results from the reiterative coactivation of pathways that are largely inactive in vegetative/green tissues, often involving the coregulation of clusters of neighboring genes and global regulation based on codon preference. This global transcriptomic reprogramming during maturation has not been observed in herbaceous annual species and may be a defining characteristic of perennial woody plants.

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“…A recent report of this important property was published by Manosalva et al (2009), in which RNA interference-based silencing of an Oryza sativa GLP (OsGLP) gene family member (on chromosome 8) was shown to be involved in resistance against two diseases, rice blast and sheath blight. A more specific example regarding the efficacy of GLP promoters in defense against pathogen has been reported by Himmelbach et al (2010). They analyzed a group of 8 paralogous promoters of the barley GLP subfamily 4 and confirmed a high transcript expression level upon pathogen attack.…”

Section: Introductionmentioning

confidence: 99%