2013
DOI: 10.1038/nature12349
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Abstract: Summary Transcription of the mammalian genome is pervasive but productive transcription outside protein-coding genes is limited by unknown mechanisms1. In particular, although RNA polymerase II (RNAPII) initiates divergently from most active gene promoters, productive elongation occurs primarily in the sense coding direction2–4. Here we show that asymmetric sequence determinants flanking gene transcription start sites (TSS) control promoter directionality by regulating promoter-proximal cleavage and polyadenyl… Show more

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Cited by 364 publications
(463 citation statements)
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“…ICP27's impact on polyadenylation from intronic PAS typically located within 1 kb of the 5′ splice site mirrors that recently observed when U1 snRNP's binding to the 5′ splice site was inhibited, also relieving its inhibition of CPSF binding to the downstream PAS (34)(35)(36). We hypothesize that ICP27 may thus interfere with U1 snRNP's binding to 5′ splice sites in the context of specific introns, through direct or indirect interaction with the C-rich sequences near the 5′ splice site (Fig.…”
Section: Splicing Inhibition Mediated By Icp27 and Cytosine-rich Sequmentioning
confidence: 60%
“…ICP27's impact on polyadenylation from intronic PAS typically located within 1 kb of the 5′ splice site mirrors that recently observed when U1 snRNP's binding to the 5′ splice site was inhibited, also relieving its inhibition of CPSF binding to the downstream PAS (34)(35)(36). We hypothesize that ICP27 may thus interfere with U1 snRNP's binding to 5′ splice sites in the context of specific introns, through direct or indirect interaction with the C-rich sequences near the 5′ splice site (Fig.…”
Section: Splicing Inhibition Mediated By Icp27 and Cytosine-rich Sequmentioning
confidence: 60%
“…In some cases, not even the product but rather the act of transcription itself provides functionality: For instance, deep-sequencing has identified the existence of a class of divergent lncRNAs that are transcribed anti-sense to the promoters of coding genes and provide directionality of coding gene transcription (Almada et al, 2013). The resulting lncRNA products might simply represent byproducts of transcription and are typically rapidly degraded after synthesis (Clark et al, 2012).…”
Section: Eukaryotic Long Non-coding Rnas (Lncrnas)mentioning
confidence: 99%
“…Furthermore, a number of studies have demonstrated roles for U1 outside of pre-mRNA splicing. These include regulating the use of cleavage and polyadenylation sites (54) and conferring proper polarity to bidirectional transcription start sites (55). Whether any of these processes are affected in C. merolae remains to be determined.…”
Section: Dead Box Proteinsmentioning
confidence: 99%