Promiscuous T cell epitope prediction of Candida albicans secretory aspartyl protienase family of proteins

Tongchusak, Songsak; Brusic, Vladimir; Chaiyaroj, Sansanee C.

doi:10.1016/j.meegid.2007.09.006

Cited by 7 publications

(6 citation statements)

References 19 publications

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“…This, combined with the large MHC specificity diversity in the human population, makes direct experimental immunology, where overlapping peptides covering the genome in question are experimentally investigated for their immunogenicity, an extremely costly process. Many studies have therefore applied in silico screening methods to the identification of T‐cell epitopes 89–95 . The majority of these studies, including even the recent ones, uses the TEPITOPE prediction algorithm for the in silico screening.…”

Section: Identification Of Cd4 Epitopes and Mhc Class II Ligandsmentioning

confidence: 99%

“…Many studies have therefore applied in silico screening methods to the identification of T-cell epitopes. [89][90][91][92][93][94][95] The majority of these studies, including even the recent ones, uses the TEPITOPE prediction algorithm for the in silico screening. This is surprising because many benchmark studies have shown that state-of-the-art data-driven methods significantly outperform the TEPI-TOPE method when it comes to identification of MHC class II ligands (see above).…”

Section: Identification Of Cd4 Epitopes and Mhc Class II Ligandsmentioning

confidence: 99%

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MHC Class II epitope predictive algorithms

et al. 2010

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Summary Major histocompatibility complex class II (MHC‐II) molecules sample peptides from the extracellular space, allowing the immune system to detect the presence of foreign microbes from this compartment. To be able to predict the immune response to given pathogens, a number of methods have been developed to predict peptide–MHC binding. However, few methods other than the pioneering TEPITOPE/ProPred method have been developed for MHC‐II. Despite recent progress in method development, the predictive performance for MHC‐II remains significantly lower than what can be obtained for MHC‐I. One reason for this is that the MHC‐II molecule is open at both ends allowing binding of peptides extending out of the groove. The binding core of MHC‐II‐bound peptides is therefore not known a priori and the binding motif is hence not readily discernible. Recent progress has been obtained by including the flanking residues in the predictions. All attempts to make ab initio predictions based on protein structure have failed to reach predictive performances similar to those that can be obtained by data‐driven methods. Thousands of different MHC‐II alleles exist in humans. Recently developed pan‐specific methods have been able to make reasonably accurate predictions for alleles that were not included in the training data. These methods can be used to define supertypes (clusters) of MHC‐II alleles where alleles within each supertype have similar binding specificities. Furthermore, the pan‐specific methods have been used to make a graphical atlas such as the MHCMotifviewer, which allows for visual comparison of specificities of different alleles.

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Section: Identification Of Cd4 Epitopes and Mhc Class II Ligandsmentioning

confidence: 99%

Section: Identification Of Cd4 Epitopes and Mhc Class II Ligandsmentioning

confidence: 99%

MHC Class II epitope predictive algorithms

et al. 2010

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“…In candidiasis, Elispot has been also used for the study of immunogenic epitopes of secretory aspartyl proteinase 2 (Sap2) [ 113 , 114 ]. Sap2 is the most abundant virulence factor expressed during Candida infection, and the principal protein known to induce antibody response during Candida infection in humans.…”

Section: Mycosismentioning

confidence: 99%

How Can Elispot Add Information to Improve Knowledge on Tropical Diseases?

Lima-Junior

Morgado

Conceição-Silva

2017

Cells

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Elispot has been used as an important tool for detecting immune cells’ products and functions and has facilitated the understanding of host-pathogen interaction. Despite the incredible diversity of possibilities, two main approaches have been developed: the immunopathogenesis and diagnosis/prognosis of infectious diseases as well as cancer research. Much has been described on the topics of allergy, autoimmune diseases, and HIV-Aids, however, Elispot can also be applied to other infectious diseases, mainly leishmaniasis, malaria, some viruses, helminths and mycosis usually classified as tropical diseases. The comprehension of the function, concentration and diversity of the immune response in the infectious disease is pointed out as crucial to the development of infection or disease in humans and animals. In this review we will describe the knowledge already obtained using Elispot as a method for accessing the profile of immune response as well as the recent advances in information about host-pathogen interaction in order to better understand the clinical outcome of a group of tropical and neglected diseases.

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“…T cell epitope is located at the C-terminal region of Sap2, which (e.g., SLAQVKYTSASSI) might stimulate the proliferation of splenocyte and then induced the production of cytokines. 34 In C. albicans infection, IgG identified epitope in amino acid position 386-390 (VKYTS) is associated with antibody production and survival. 35 In previously study, a hybrid phage displaying VKYTS was produced, which was found to have the ability to induce and recognize anti-Sap antibody and could be used to detect C. albicans infection.…”

Section: Disussionmentioning

confidence: 99%

Hybrid phage displaying SLAQVKYTSASSI induces protection againstCandida albicanschallenge in BALB/c mice

Wang

Quan-ping

Dong

et al. 2014

Human Vaccines & Immunotherapeutics

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The polymorphic fungus Candida albicans (C. albicans) can live as an aggressive pathogen and cause many diseases in hosts, for which no effective vaccine exists. The secreted aspartyl proteinase 2 (Sap2) plays a protective role in systemically infected BALB/c mice. Protective cellular immune responses can be preferentially induced when antigens are displayed on small particles. Therefore, the emphasis is placed on developing new phage vaccine to inhibit C. albicans infection. In this study, the ability of the hybrid phage displaying the epitope SLAQVKYTSASSI and recombinant protein of Sap2 (rSap2) for inducing immune protective responses against C. albicans infection was evaluated by lymphoproliferative assay, to gather cytokine and antibody measurements in BALB/c mice. Our results showed that, strong cellular and humoral immune responses were induced in a mouse model immunized with hybrid phage or rSap2. Furthermore, the protection against lethal challenge with C. albicans was observed in mice vaccinated hybrid phage without adjuvant. These findings demonstrate that the hybrid phage displaying the epitope SLAQVKYTSASSI might be a potential vaccine against C. albicans infections.

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Promiscuous T cell epitope prediction of Candida albicans secretory aspartyl protienase family of proteins

Cited by 7 publications

References 19 publications

MHC Class II epitope predictive algorithms

MHC Class II epitope predictive algorithms

How Can Elispot Add Information to Improve Knowledge on Tropical Diseases?

Hybrid phage displaying SLAQVKYTSASSI induces protection againstCandida albicanschallenge in BALB/c mice

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