Prolonged 24-hour subzero preservation of heterotopically transplanted rat hearts using antifreeze proteins derived from arctic fish

Amir, Gabriel; Rubinsky, Boris; Horowitz, Liana; Miller, Liron; Leor, Jonathan; Kassif, Yigal; Mishaly, David; Smolinsky, Aram; Lavee, Jacob

doi:10.1016/j.athoracsur.2003.04.004

Cited by 46 publications

(33 citation statements)

References 10 publications

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“…The feasibility of optimizing and translating supercooling preservation to hearts is supported by the fact that Amir et al [92-94] have reported rat heart preservation 7 times longer than with conventional methods using a solution containing only antifreeze proteins and no other cryoprotectants, as well as by our own results with nonfrozen heart preservation under pressure discussed further below. These early achievements in supercooling preservation also highlight some of the nature-inspired strategies that contributed to the demonstrated successes outlined here.…”

Section: High-subzero Preservationsupporting

confidence: 48%

New Approaches to Cryopreservation of Cells, Tissues, and Organs

Taylor¹,

Weegman²,

Baicu³

et al. 2019

Transfus Med Hemother

116

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In this concept article, we outline a variety of new approaches that have been conceived to address some of the remaining challenges for developing improved methods of biopreservation. This recognizes a true renaissance and variety of complimentary, high-potential approaches leveraging inspiration by nature, nanotechnology, the thermodynamics of pressure, and several other key fields. Development of an organ and tissue supply chain that can meet the healthcare demands of the 21st century means overcoming twin challenges of (1) having enough of these lifesaving resources and (2) having the means to store and transport them for a variety of applications. Each has distinct but overlapping logistical limitations affecting transplantation, regenerative medicine, and drug discovery, with challenges shared among major areas of biomedicine including tissue engineering, trauma care, transfusion medicine, and biomedical research. There are several approaches to biopreservation, the optimum choice of which is dictated by the nature and complexity of the tissue and the required length of storage. Short-term hypothermic storage at temperatures a few degrees above the freezing point has provided the basis for nearly all methods of preserving tissues and solid organs that, to date, have proved refractory to cryopreservation techniques successfully developed for single-cell systems. In essence, these short-term techniques have been based on designing solutions for cellular protection against the effects of warm and cold ischemia and basically rely upon the protective effects of reduced temperatures brought about by Arrhenius kinetics of chemical reactions. However, further optimization of such preservation strategies is now seen to be restricted. Long-term preservation calls for much lower temperatures and requires the tissue to withstand the rigors of heat and mass transfer during protocols designed to optimize cooling and warming in the presence of cryoprotective agents. It is now accepted that with current methods of cryopreservation, uncontrolled ice formation in structured tissues and organs at subzero temperatures is the single most critical factor that severely restricts the extent to which tissues can survive procedures involving freezing and thawing. In recent years, this major problem has been effectively circumvented in some tissues by using ice-free cryopreservation techniques based upon vitrification. Nevertheless, despite these promising advances there remain several recognized hurdles to be overcome before deep-subzero cryopreservation, either by classic freezing and thawing or by vitrification, can provide the much-needed means for biobanking complex tissues and organs for extended periods of weeks, months, or even years. In many cases, the approaches outlined here, including new underexplored paradigms of high-subzero preservation, are novel and inspired by mechanisms of freeze tolerance, or freeze avoidance, in nature. Others apply new bioengineering techniques such as nanotechnology, isochoric pressure preserv...

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Section: High-subzero Preservationsupporting

confidence: 48%

New Approaches to Cryopreservation of Cells, Tissues, and Organs

Taylor¹,

Weegman²,

Baicu³

et al. 2019

Transfus Med Hemother

116

View full text Add to dashboard Cite

show abstract

“…Although there have been limited data about organ preservation solutions used in the supercooling condition, such as ET-Kyoto solution, University of Wisconsin solution, 8,9 and Euro-Collins solution, 13 these solutions have a tendency to freeze in sub-zero temperatures without a supercooling refrigerator or anti-freezing proteins, 8,9 because of a restricted effect of freezing point depression. In this investigation, the sub-zero preservation was done using ET-Kyoto solution, which contains trehalose, a disaccharide known as a cryoprotectant.…”

Section: Discussionmentioning

confidence: 99%

“…2 In the natural world, many creatures live or hibernate below 0°C: frogs at Ϫ6°C with glucose as a cryoprotectant, 3 some types of fish at Ϫ2°C with anti-freezing proteins, 4 and squirrels at Ϫ2.9°C. 5 Besides the historical success in the preservation of cells and tissues in a frozen state, several trials of organ preservation at sub-zero temperatures have been reported using cryoprotectants such as polyethylene glycol 6 and 2-3 butanediol 7 or anti-freezing proteins 8,9 to avoid cell damage. However, these techniques have not been successful in the heart, 6 liver, 7 and kidney, 10 although higher levels of high-energy phosphate compounds and lower levels of deviation enzymes have been reported at sub-zero temperatures.…”

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confidence: 99%

Successful Sub-zero Non-freezing Preservation of Rat Lungs at −2°C Utilizing a New Supercooling Technology

Okamoto

Nakamura

Zhang

et al. 2008

The Journal of Heart and Lung Transplantation

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“…Furthermore, the fundamental insights into the unique ability of IBPs to tune ice crystal growth have seen little translation into reallife applications despite promising preliminary results in frozen foods, 14,15 gas hydrate inhibition, [159][160][161] and the cryopreservation of cells, tissues, and organs. 16 We foresee novel insights from single-molecule techniques aiming to FIG. 17.…”

Section: Discussionmentioning

confidence: 99%

“…12,13 The unique ability of IBPs to modify ice crystal growth also holds great promise for a range of application areas including food technology, materials science, and biomedicine. [14][15][16] In this paper, we review major advances in the field of IBPs focusing in particular on recent experimental and theoretical studies aiming to elucidate how IBPs function. We first give an overview of the structure and function of IBPs, highlighting new developments in activity and ice-binding plane assays.…”

Section: Introductionmentioning

confidence: 99%

Interaction of ice binding proteins with ice, water and ions

et al. 2016

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Articles you may be interested inExplicit-water theory for the salt-specific effects and Hofmeister series in protein solutions J. Chem. Phys. 144, 215101 (2016) Ice binding proteins (IBPs) are produced by various cold-adapted organisms to protect their body tissues against freeze damage. First discovered in Antarctic fish living in shallow waters, IBPs were later found in insects, microorganisms, and plants. Despite great structural diversity, all IBPs adhere to growing ice crystals, which is essential for their extensive repertoire of biological functions. Some IBPs maintain liquid inclusions within ice or inhibit recrystallization of ice, while other types suppress freezing by blocking further ice growth. In contrast, ice nucleating proteins stimulate ice nucleation just below 0 C. Despite huge commercial interest and major scientific breakthroughs, the precise working mechanism of IBPs has not yet been unraveled. In this review, the authors outline the state-of-the-art in experimental and theoretical IBP research and discuss future scientific challenges. The interaction of IBPs with ice, water and ions is examined, focusing in particular on ice growth inhibition mechanisms.

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Prolonged 24-hour subzero preservation of heterotopically transplanted rat hearts using antifreeze proteins derived from arctic fish

Cited by 46 publications

References 10 publications

New Approaches to Cryopreservation of Cells, Tissues, and Organs

New Approaches to Cryopreservation of Cells, Tissues, and Organs

Successful Sub-zero Non-freezing Preservation of Rat Lungs at −2°C Utilizing a New Supercooling Technology

Interaction of ice binding proteins with ice, water and ions

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