Proline dehydrogenase-entrapped mesoporous magnetic silica nanomaterial for electrochemical biosensing of L-proline in biological fluids

Hasanzadeh, Mohammad; Nahar, Arezoo Saadati; Hassanpour, Soodabeh; Shadjou, Nasrin; Mokhtarzadeh, Ahad; Mohammadi, Jalal

doi:10.1016/j.enzmictec.2017.05.007

Cited by 59 publications

(27 citation statements)

References 35 publications

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“…Therefore, the interactions between enzyme and support are potentially affected by the distinct mechanical, chemical, biochemical, and kinetic properties . In recent past, researchers have adapted porous nanomaterials as an alternative support for immobilization by employing various types of physical and chemical processes, including enzyme adsorption, entrapment, and encapsulation in or on polymer supports. Apart from this, enzymes covalently bound to an organic or inorganic polymer support have also been widely studied .…”

Section: Introductionmentioning

confidence: 99%

Rapid In Situ Encapsulation of Laccase into Metal‐Organic Framework Support (ZIF‐8) under Biocompatible Conditions

Patil

Yadav

2018

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In situ immobilization of laccase into metal‐organic framework (laccase‐MOF) was performed by assisting one‐pot synthesis strategy. A novel method to synthesize octahedral laccase‐MOF composite with enhanced thermostability is reported. The present work deals with entrapment of laccase into zeolitic imidazolate framework (ZIF‐8) by simply mixing solution of zinc acetate, 2‐methylimidazole, and laccase at room temperature (28 ± 2 °C). Fourier transform‐infrared spectroscopy (FT‐IR), Scanning electron microscopy (SEM), Thermogravimetric analysis (TGA), and Confocal scanning microscopy were employed to characterize laccase‐MOF composite. The thermostability of prepared composite was assessed concerning half‐life at varying temperatures which conferred 3.6‐fold enhancement over free enzyme. Also, kinetic studies of immobilized laccase showed a slightly higher Km and lower Vmax values along with the enhancement of thermodynamic parameters after non‐covalent interaction. Moreover, the immobilized laccase‐MOF maintained up to 48% of residual activity for seven subsequent cycles in batch mode, whereas the storage stability studies exhibited up to 83% up to 21 days of storage. Finally, FT‐IR data tool was employed to investigate the effect of encapsulation on fractions of secondary structure of protein.

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Section: Introductionmentioning

confidence: 99%

Rapid In Situ Encapsulation of Laccase into Metal‐Organic Framework Support (ZIF‐8) under Biocompatible Conditions

Patil

Yadav

2018

ChemistrySelect

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“…Its slope is 0.223 and indicates diffusion control of the electrode process. A slope close to 0.5 is expected for diffusion‐controlled electrode processes and close to 1.0 for adsorption‐controlled processes …”

Section: Methodsmentioning

confidence: 99%

“…A slope close to 0.5 is expected for diffusion-controlled electrode processes and close to 1.0 for adsorption-controlled processes. [44][45][46][47] Figure S2C presents a dependence of E pa on scan rate determined from cyclic voltammogram recorded for the MDA electrooxidation. If electrochemical reaction is irreversible, then E pa is independent on v.…”

Section: Gqds-gce Exhibits Superior Performance For Mda Oxidation Thanmentioning

confidence: 99%

Electrochemical monitoring of malondialdehyde biomarker in biological samples via electropolymerized amino acid/chitosan nanocomposite

Hasanzadeh

Mokhtari

Jouyban-Gharamaleki

et al. 2018

J of Molecular Recognition

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This study reports on the electropolymerization of a low toxic and biocompatible nanopolymer with entitle poly arginine-graphene quantum dots-chitosan (PARG-GQDs-CS) as a novel strategy for surface modification of glassy carbon surface and preparation of a new interface for measurement of malondialdehyde (MDA) in exhaled breath condensate. Electrochemical deposition, as a well-controlled synthesis procedure, has been used for subsequently layer-by-layer preparation of GQDs-CS nanostructures on a PARG prepolymerized on the surface of glassy carbon electrode using cyclic voltammetry techniques in the regime of -1.5 to 2 V. The modified electrode appeared as an effective electroactivity for detection of MDA by using cyclic voltammetry, linear sweep voltammetry, and differential pulse voltammetry. The prepared modified electrode demonstrated a noticeably good activity for electrooxidation of MDA than PARG. Enhancement of peak currents is ascribed to the fast heterogeneous electron transfer kinetics that arise from the synergistic coupling between the excellent properties of PARG and semiconducting polymer, GQDs as high density of edge plane sites and subtle electronic characteristics and unique properties of CS such as excellent film-forming ability, high permeability, good adhesion, nontoxicity, cheapness, and a susceptibility to chemical modification. The prepared sensor showed 1 oxidation processes for MDA at potentials about 1 V with a low limit of quantification 5.94 nM. Finally, application of new sensor for determination of MDA in exhaled breath condensate was suited. In general, the simultaneous attachment of GQDs and CS to structure of poly amino acids provides new opportunities within the personal healthcare.

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“…One of the best candidates for this purpose is electrochemical sensing. 17 In this work, as a continuation of our previous works in the construction of electrochemical sensors for amino acids [18][19][20][21][22][23][24][25][26][27][28][29][30] and considering the benefits of the GQDs, GNPs, and P-Cys, we used these materials for the preparation of a novel electrochemical sensor for the detection of L-Pro in real samples. Electrochemical behavior of the prepared electrode was characterized by using cyclic voltammetry (CV), differential pulse voltammetry (DPV) and the chronoamperometry (CHA) technique.…”

Section: Introductionmentioning

confidence: 99%

Non-enzymatic Determination of L-Proline Amino Acid in Unprocessed Human Plasma Sample Using Hybrid of Graphene Quantum Dots Decorated with Gold Nanoparticles and Poly Cysteine: A Novel Signal Amplification Strategy

2018

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An innovative electrochemical interface for quantitation of L-proline (L-Pro) based on ternary amplification strategy was fabricated. In this work, gold nanoparticles prepared by soft template methodology were immobilized onto green and biocompatible nanocomposite containing poly as a conductive matrix and graphene quantum dots as the amplification element. Therefore, a novel multilayer film based on poly-L-cysteine, graphene quantum dots (GQDs), and gold nanoparticles (GNPs) was exploited to develop a highly sensitive electrochemical sensor for the detection of L-Pro. Fully electrochemical methodology was used to prepare a new transducer on a glassy carbon electrode, which provided a high surface area towards sensitive detection of L-Pro. The prepared electrode was employed for the detection of L-Pro. Under optimized conditions, the calibration curve for L-Pro concentration was linear in 0.5 nM - 10 mM with a low limit of quantification of 0.1 nM. The practical analytical utility of the modified electrode was illustrated by determination of L-Pro in unprocessed human plasma samples.

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Proline dehydrogenase-entrapped mesoporous magnetic silica nanomaterial for electrochemical biosensing of L-proline in biological fluids

Cited by 59 publications

References 35 publications

Rapid In Situ Encapsulation of Laccase into Metal‐Organic Framework Support (ZIF‐8) under Biocompatible Conditions

Rapid In Situ Encapsulation of Laccase into Metal‐Organic Framework Support (ZIF‐8) under Biocompatible Conditions

Electrochemical monitoring of malondialdehyde biomarker in biological samples via electropolymerized amino acid/chitosan nanocomposite

Non-enzymatic Determination of L-Proline Amino Acid in Unprocessed Human Plasma Sample Using Hybrid of Graphene Quantum Dots Decorated with Gold Nanoparticles and Poly Cysteine: A Novel Signal Amplification Strategy

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