Proliferative Vitreoretinopathy: Immunoreactive Endothelin-1

Preoperative duration of retinal detachment and subretinal immunoreactive endothelin-1: repercussion on logarithmic visual acuity

“…The circulatory compromise was found greater in PVR cases and related with preoperative RD duration [8]. In addition to having found higher intraocular IR-ET-1 levels [13,14] in RD patients with PVR than in those without PVR, we localized ET-1 by immunohistochemical analysis of epiretinal proliferative tissue from PVR membranes and secretion of ET-1 by glial and RPE cells. In addition to that, expression of ETA and ETB receptors were demonstrated in these membranes [14].…”

“…Best-corrected LogMAR VA and retinal status were recorded on the day before and 8 months after surgery for our study Venous blood samples (10 ml) were obtained prior to the anesthetic procedure for the scleral buckling surgery. SRF was obtained during the drainage procedure of retinal reattachment surgery [17], as previously reported [7,13]. Briefly, after 1 mm sclerotomy and local diathermic coagulation of the choroid at the site of maximum prominence of the detached retina, a plastic disposable fire tip transfer pipet (Life Scan Inc., Milpitas, CA, USA) was used to obtain the SRF.…”

“…Briefly, after 1 mm sclerotomy and local diathermic coagulation of the choroid at the site of maximum prominence of the detached retina, a plastic disposable fire tip transfer pipet (Life Scan Inc., Milpitas, CA, USA) was used to obtain the SRF. Both SRF and blood samples were collected in chilled tubes containing protease inhibitors [7,13,14]. Blood samples were centrifuged at 3,000 rpm for 20 min at 4°C (plasma).…”

“…The ET-1 extraction with Sep-Pack C18 cartridge (Waters Associates, Milford, MA, USA) and IR-ET-1 measurements were carried out in plasma and SRF samples as previously reported [7,13,14]. Briefly, IR-ET-1 was assayed in the laboratory by RIA, using polyclonal antibody against synthetic ET-1 (Peninsula Laboratories, Merseyside, UK) at a 1:90,000 final dilution.…”

“…Experimental evidence that stimulation of endothelinergic receptors may modulate photoreceptor survival and glial activation has been provided [10], and reactive gliosis on detachment has been suggested as a clinically significant limiting factor in the recovery of vision after reattachment [11,12]. Previously we have reported the relationship between increased immunoreactive ET-1 (IR-ET-1) levels in the eye [in the vitreous and in subretinal fluid (SRF)] and the proliferative complications of retinal detachment [13,14]. The higher the IR-ET-1 levels the lower the postoperative VA, and the lower the VA difference (postoperative 8 months minus preoperative) in RD [7].…”