Proliferative responses of quail aortic smooth muscle cells to benzo[a]pyrene: implications in PAH-induced atherogenesis

Identification of Benzo[a]pyrene-InducibleCis-Acting Elements Within c-Ha-rasTranscriptional Regulatory Sequences

“…2 extend our work in heterologous species to mouse SMCs. A near-identical pattern of expression for c-Ha-ras in response to BaP challenge has been observed in both quail and rat SMCs (14,19), as well as in rat hepatocytes (30). These findings suggest that the response of Ha-ras transcription to BaP extends across species lines and may be a common effect in higher eukaryotes.…”

“…BaP has been characterized as both an initiator and a promoter of atherogenesis (10). Treatment of SMCs with BaP results in DNA adduct formation (37), disruption of DNA replication (19), and alterations in protein kinase C activity (38). These pleiotropic effects are indicative of the complex nature of cellular responses elicited by this chemical.…”

“…Cell culture. Primary cultures of SMCs were isolated from inbred C57BL/6 mouse aorta and maintained under standard conditions as described previously (19). Cells were grown in Media 199 (GIBCO, Grand Island, NY) containing 10% FBS (Atlanta Biologicals, Norcross, GA), 2 mM glutamine, 100 units/ml penicillin, 0.1 mg/ml streptomycin, and 0.25 g/ml amphotericin B (GIBCO).…”

Identification of Benzo[a]pyrene-InducibleCis-Acting Elements Within c-Ha-rasTranscriptional Regulatory Sequences

“…2 extend our work in heterologous species to mouse SMCs. A near-identical pattern of expression for c-Ha-ras in response to BaP challenge has been observed in both quail and rat SMCs (14,19), as well as in rat hepatocytes (30). These findings suggest that the response of Ha-ras transcription to BaP extends across species lines and may be a common effect in higher eukaryotes.…”

“…BaP has been characterized as both an initiator and a promoter of atherogenesis (10). Treatment of SMCs with BaP results in DNA adduct formation (37), disruption of DNA replication (19), and alterations in protein kinase C activity (38). These pleiotropic effects are indicative of the complex nature of cellular responses elicited by this chemical.…”

“…Cell culture. Primary cultures of SMCs were isolated from inbred C57BL/6 mouse aorta and maintained under standard conditions as described previously (19). Cells were grown in Media 199 (GIBCO, Grand Island, NY) containing 10% FBS (Atlanta Biologicals, Norcross, GA), 2 mM glutamine, 100 units/ml penicillin, 0.1 mg/ml streptomycin, and 0.25 g/ml amphotericin B (GIBCO).…”

Hydroperoxides and cytokines as biomarkers in detecting atherosclerosis predisposition in cigarette smokers

“…In animal models, it has been shown that a brief exposure to cigarette smoke is able to promote atherosclerotic plaque development [29], and the combination of second hand smoke and hypercholesterolemia resulted, to a greater extent, in mtDNA (mitochondrial DNA) damage and atherosclerosis [30]. In addition, it has been documented that benzo[a]pyrene, a polycyclic aromatic hydrocarbon present in tobacco smoke, can modulate VSMCs from a quiescent to a proliferative phenotype both in vitro and in vivo [31,32].…”

Aberrant Gene Expression and Cell Signalling/Epigenetic Effects Induced by Polycyclic Aromatic Hydrocarbons

“…93 " 97 In addition, B[a]P-DNA adducts have been detected in atherosclerotic arteries. 96,98 ' 99 Regarding the mechanisms involved in the deregulation of vascular smooth muscle cell growth and differentiation during the course of atherogenesis and the role of PAHs in this process, Ou et al 10° reported that exposure of quail smooth muscle cells to B[a]P inhibited inositol phospholipid turnover and protein kinase C activity and concluded that the ability of B[a]P to modulate growth and differentiation in quail aortic smooth muscle cells involved early interference with protein kinase C-related signal transduction.…”

Identification of Benzo[a]pyrene-InducibleCis-Acting Elements Within c-Ha-rasTranscriptional Regulatory Sequences

“…2 extend our work in heterologous species to mouse SMCs. A near-identical pattern of expression for c-Ha-ras in response to BaP challenge has been observed in both quail and rat SMCs (14,19), as well as in rat hepatocytes (30). These findings suggest that the response of Ha-ras transcription to BaP extends across species lines and may be a common effect in higher eukaryotes.…”

“…BaP has been characterized as both an initiator and a promoter of atherogenesis (10). Treatment of SMCs with BaP results in DNA adduct formation (37), disruption of DNA replication (19), and alterations in protein kinase C activity (38). These pleiotropic effects are indicative of the complex nature of cellular responses elicited by this chemical.…”

“…Cell culture. Primary cultures of SMCs were isolated from inbred C57BL/6 mouse aorta and maintained under standard conditions as described previously (19). Cells were grown in Media 199 (GIBCO, Grand Island, NY) containing 10% FBS (Atlanta Biologicals, Norcross, GA), 2 mM glutamine, 100 units/ml penicillin, 0.1 mg/ml streptomycin, and 0.25 g/ml amphotericin B (GIBCO).…”

Hydroperoxides and cytokines as biomarkers in detecting atherosclerosis predisposition in cigarette smokers

“…In animal models, it has been shown that a brief exposure to cigarette smoke is able to promote atherosclerotic plaque development [29], and the combination of second hand smoke and hypercholesterolemia resulted, to a greater extent, in mtDNA (mitochondrial DNA) damage and atherosclerosis [30]. In addition, it has been documented that benzo[a]pyrene, a polycyclic aromatic hydrocarbon present in tobacco smoke, can modulate VSMCs from a quiescent to a proliferative phenotype both in vitro and in vivo [31,32].…”

Aberrant Gene Expression and Cell Signalling/Epigenetic Effects Induced by Polycyclic Aromatic Hydrocarbons

“…93 " 97 In addition, B[a]P-DNA adducts have been detected in atherosclerotic arteries. 96,98 ' 99 Regarding the mechanisms involved in the deregulation of vascular smooth muscle cell growth and differentiation during the course of atherogenesis and the role of PAHs in this process, Ou et al 10° reported that exposure of quail smooth muscle cells to B[a]P inhibited inositol phospholipid turnover and protein kinase C activity and concluded that the ability of B[a]P to modulate growth and differentiation in quail aortic smooth muscle cells involved early interference with protein kinase C-related signal transduction.…”

Identification of Benzo[a]pyrene-InducibleCis-Acting Elements Within c-Ha-rasTranscriptional Regulatory Sequences

“…2 extend our work in heterologous species to mouse SMCs. A near-identical pattern of expression for c-Ha-ras in response to BaP challenge has been observed in both quail and rat SMCs (14,19), as well as in rat hepatocytes (30). These findings suggest that the response of Ha-ras transcription to BaP extends across species lines and may be a common effect in higher eukaryotes.…”

“…BaP has been characterized as both an initiator and a promoter of atherogenesis (10). Treatment of SMCs with BaP results in DNA adduct formation (37), disruption of DNA replication (19), and alterations in protein kinase C activity (38). These pleiotropic effects are indicative of the complex nature of cellular responses elicited by this chemical.…”

“…Cell culture. Primary cultures of SMCs were isolated from inbred C57BL/6 mouse aorta and maintained under standard conditions as described previously (19). Cells were grown in Media 199 (GIBCO, Grand Island, NY) containing 10% FBS (Atlanta Biologicals, Norcross, GA), 2 mM glutamine, 100 units/ml penicillin, 0.1 mg/ml streptomycin, and 0.25 g/ml amphotericin B (GIBCO).…”

Hydroperoxides and cytokines as biomarkers in detecting atherosclerosis predisposition in cigarette smokers

“…In animal models, it has been shown that a brief exposure to cigarette smoke is able to promote atherosclerotic plaque development [29], and the combination of second hand smoke and hypercholesterolemia resulted, to a greater extent, in mtDNA (mitochondrial DNA) damage and atherosclerosis [30]. In addition, it has been documented that benzo[a]pyrene, a polycyclic aromatic hydrocarbon present in tobacco smoke, can modulate VSMCs from a quiescent to a proliferative phenotype both in vitro and in vivo [31,32].…”

Aberrant Gene Expression and Cell Signalling/Epigenetic Effects Induced by Polycyclic Aromatic Hydrocarbons

“…93 " 97 In addition, B[a]P-DNA adducts have been detected in atherosclerotic arteries. 96,98 ' 99 Regarding the mechanisms involved in the deregulation of vascular smooth muscle cell growth and differentiation during the course of atherogenesis and the role of PAHs in this process, Ou et al 10° reported that exposure of quail smooth muscle cells to B[a]P inhibited inositol phospholipid turnover and protein kinase C activity and concluded that the ability of B[a]P to modulate growth and differentiation in quail aortic smooth muscle cells involved early interference with protein kinase C-related signal transduction.…”