Proliferation effects of phenylboronic acid and boric acid on canine peripheral blood mononuclear cells

Abstract

The effects of phenylboronic acid (PBA) and boric acid (BA) on the proliferation of canine peripheral blood mononuclear cells (PBMCs) were investigated. Treatment of PBMCs from mixed breed dogs with PBA or BA (0.05 µg/mL) alone elicited blastogenic responses of 0.478 ± 0.438 and 0.499 ± 0.366, respectively. We observed an additive effect on proliferation when purebred Kangal PBMCs were cultured in a combination of BA (0.001 µg/mL) and the mitogenic lectin concanavalin A (1 µg/mL). However, the effects were not statistically significant. We detected varied IL-2 and IL-4 secretion in response to stimulation with boron-containing compounds. Our findings suggest that PBA induces canine PBMC proliferation, albeit less potently than lectins. However, boronic acid compounds may still prove beneficial as adjuvants to elicit cell proliferation and boost canine immune responses.

Phenylboronic Acid Conjugated to Doxorubicin for the Treatment of Hepatocellular Carcinoma Through Transcatheter Arterial Chemoembolization

“…After TACE in the pPBA-Dox nanocomplexes and Dox groups, it was con rmed through H&E staining that tumor necrosis occurred and immune cells in ltrated around the tumor. In addition, there is a report that PBA used as a drug delivery agent also affects the proliferation of PBMCs (30). Therefore, we con rmed the change in the T cell population in the liver after TACE to con rm the possibility of ICIs after TACE.…”