Proliferation and morphological transformation of BALB/3T3 cells by a prolonged treatment with sodium orthovanadate

Detection of Cell Carcinogenic Transformation by a Quadruplex DNA Binding Fluorescent Probe

“…Cells treated by MCA were dense, multilayered, and randomly oriented. The morphology of cell became elongated and spindle (Figure 1b) [38]. In subcellular level, BALB/c 3T3 cells usually exhibited numerous stress fibers and nonpolarized cell shapes.…”

“…Unlimited cell proliferation is an important characteristic of cell transformation during carcinogenesis [3], [4], [38], [44]. In this study, cell proliferation was determined by BrdU incorporation.…”

Detection of Cell Carcinogenic Transformation by a Quadruplex DNA Binding Fluorescent Probe

“…Cells treated by MCA were dense, multilayered, and randomly oriented. The morphology of cell became elongated and spindle (Figure 1b) [38]. In subcellular level, BALB/c 3T3 cells usually exhibited numerous stress fibers and nonpolarized cell shapes.…”

“…Unlimited cell proliferation is an important characteristic of cell transformation during carcinogenesis [3], [4], [38], [44]. In this study, cell proliferation was determined by BrdU incorporation.…”

Vanadyl sulfate can differentially damage DNA in human lymphocytes and HeLa cells

“…On the one hand, vanadyl sulfate and orthovanadate can stimulate DNA synthesis in mammary glands (Hori and Oka 1980;Gallo-Hendrikx et al 2001), 3T3 and 3T6 cells (Smith 1983;Sheu et al 1992), and BALB/3T3 cells (Cortizo et al 1997). It seems that vanadium salts have biphasic effect on DNA synthesis and, by this way, on proliferation and differentiation.…”

Growth stimulation of primary B cell precursors by the anti-phosphatase Sbf1

“…Many dsPTPases are nuclear and some have been shown to specifically dephosphorylate MAP kinases to oppose mitogenic signaling pathways. The anti-oncogenic functions of protein phosphatases are evidenced by the ability of phosphatase inhibitor sodium orthovanadate to transform fibroblasts in vitro (43,44). Recently, the dsPTPase PTEN (also known as MMAC1) has been shown to be mutated or deleted in several human cancers (45)(46)(47).…”

Detection of Cell Carcinogenic Transformation by a Quadruplex DNA Binding Fluorescent Probe

“…Cells treated by MCA were dense, multilayered, and randomly oriented. The morphology of cell became elongated and spindle (Figure 1b) [38]. In subcellular level, BALB/c 3T3 cells usually exhibited numerous stress fibers and nonpolarized cell shapes.…”

“…Unlimited cell proliferation is an important characteristic of cell transformation during carcinogenesis [3], [4], [38], [44]. In this study, cell proliferation was determined by BrdU incorporation.…”

Vanadyl sulfate can differentially damage DNA in human lymphocytes and HeLa cells

“…On the one hand, vanadyl sulfate and orthovanadate can stimulate DNA synthesis in mammary glands (Hori and Oka 1980;Gallo-Hendrikx et al 2001), 3T3 and 3T6 cells (Smith 1983;Sheu et al 1992), and BALB/3T3 cells (Cortizo et al 1997). It seems that vanadium salts have biphasic effect on DNA synthesis and, by this way, on proliferation and differentiation.…”

Growth stimulation of primary B cell precursors by the anti-phosphatase Sbf1

“…Many dsPTPases are nuclear and some have been shown to specifically dephosphorylate MAP kinases to oppose mitogenic signaling pathways. The anti-oncogenic functions of protein phosphatases are evidenced by the ability of phosphatase inhibitor sodium orthovanadate to transform fibroblasts in vitro (43,44). Recently, the dsPTPase PTEN (also known as MMAC1) has been shown to be mutated or deleted in several human cancers (45)(46)(47).…”

Detection of Cell Carcinogenic Transformation by a Quadruplex DNA Binding Fluorescent Probe

“…Cells treated by MCA were dense, multilayered, and randomly oriented. The morphology of cell became elongated and spindle (Figure 1b) [38]. In subcellular level, BALB/c 3T3 cells usually exhibited numerous stress fibers and nonpolarized cell shapes.…”

“…Unlimited cell proliferation is an important characteristic of cell transformation during carcinogenesis [3], [4], [38], [44]. In this study, cell proliferation was determined by BrdU incorporation.…”

Vanadyl sulfate can differentially damage DNA in human lymphocytes and HeLa cells

“…On the one hand, vanadyl sulfate and orthovanadate can stimulate DNA synthesis in mammary glands (Hori and Oka 1980;Gallo-Hendrikx et al 2001), 3T3 and 3T6 cells (Smith 1983;Sheu et al 1992), and BALB/3T3 cells (Cortizo et al 1997). It seems that vanadium salts have biphasic effect on DNA synthesis and, by this way, on proliferation and differentiation.…”

Growth stimulation of primary B cell precursors by the anti-phosphatase Sbf1

“…Many dsPTPases are nuclear and some have been shown to specifically dephosphorylate MAP kinases to oppose mitogenic signaling pathways. The anti-oncogenic functions of protein phosphatases are evidenced by the ability of phosphatase inhibitor sodium orthovanadate to transform fibroblasts in vitro (43,44). Recently, the dsPTPase PTEN (also known as MMAC1) has been shown to be mutated or deleted in several human cancers (45)(46)(47).…”