Proliferation and characterization of Wharton-jelly's derived mesenchymal stromal cell using plasma rich platelet and plasma platelet lysate

Chouw, Angliana; Dirgantara, Y.; Putera, Bayu Winata; Sartika, C.R.; Meutia, D.; Yuliana, P.; Sandra, Ferry

doi:10.1016/j.jcyt.2017.02.230

Cited by 4 publications

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“…13 The application of stem cell therapy in the medical field are rapidly developing. Efforts to achieve the applications also vary, such as the development of stem cell and its microenvironment isolation methods 14,15 , development of methods to increase the number of stem cells [16][17][18][19][20][21][22] , the development of stem cell differentiation methods 23 (including cell reprogramming) 24,25 , stem cell storage methods 26,27 , the development of stem cell transplantation methods 16,28 , etc. Since then, some particular stem cell types with their potential differentiation capacities can be isolated and cultured to meet the requirement of each clinical study to be explored.…”

Section: B I O P H a R M A C E U T I C A L I N S T I T U T Ementioning

confidence: 99%

“…Meanwhile, in some circumstances, cell number is not enough, so therefore cell number should be enriched in an in vitro stem cell culture setting. 16,17,[19][20][21][22] In an in vitro stem cell culture, besides suitable and sterile equipment, reagents such as culture medium, serum and antibiotics are all important. Although all those criteria are fulfilled, somehow stem cell enrichment cannot be achieved, cell number is still below the target.…”

Section: B I O P H a R M A C E U T I C A L I N S T I T U T Ementioning

confidence: 99%

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Role of Herbal Extract in Stem Cell Development

Sandra

2018

Mol Cell Biomed Sci

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Stem cell research has been developed and today we can witness some stem cell clinical trials are on going in Indonesia. To meet a successful stem cell treatment, several factors need to be considered, such as cell number. Cell number has been reported to be crucial, and therefore optimal cell number should be achieved. Meanwhile, in some circumstances, cell number is not enough, therefore, cell number should be enriched in an in vitro stem cell culture setting. In an in vitro stem cell culture, besides suitable and sterile equipment, reagents such as culture medium, serum and antibiotics are all important. Although all those criteria are fulfilled, somehow stem cell enrichment cannot be achieved, cell number is still below the target. Modification of stem cell microenvironment should then be an alternative. The addition of growth factors is a part of the strategies to reach a better enrichment. So that, stem cells could later be induced to proliferate at a higher rate. This strategy was then pursued by the scientist involved in herbal medicine. Herbal extracts were now highly investigated due to its potential to induce cell proliferation. Some herbal extracts inducing proliferation and differentiation of stem cell will be shown and described.Keywords: herbal extract, stem cell, progenitor cell, proliferation, differentiation

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Section: B I O P H a R M A C E U T I C A L I N S T I T U T Ementioning

confidence: 99%

Section: B I O P H a R M A C E U T I C A L I N S T I T U T Ementioning

confidence: 99%

Role of Herbal Extract in Stem Cell Development

Sandra

2018

Mol Cell Biomed Sci

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Anti-cancer potency and mechanism of human umbilical cord blood stem cell

Sandra¹

2019

AIP Conference Proceedings

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Conditioned Media of Human Umbilical Cord Blood Mesenchymal Stem Cell Inhibits Ultraviolet B-induced Apoptosis in Fibroblasts

Dewi¹,

Sandra

2019

Indones Biomed J

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BACKGROUND: Ultraviolet (UV)B irradiated-skin cells may respond either by activating protective or apoptotic mechanisms. Several treatments has been reported to prevent apoptosis of the skin cell. To date, despite of the cell, secretome of mesenchymal stem cell (MSC) has been progressively explored for various treatment. Current research was conducted to find out the possible effect of MSC's secretome to protect normal cells from the UVB irradiation.METHODS: For preparation of the conditioned media human umbilical cord blood mesenchymal stem cell (CM-hUCB-MSC), hUCB was collected and separated to collect mononuclear cell (MNC). MNC was cultured in MSC medium until passage 4, then checked for MSC biomarkers. Conditioned media was then produced from the cultured MSC. For induction of apoptosis, NIH3T3 cells were pretreated with/without CM-hUCB-MSC, prior to the UVB irradiation for 5-60 minutes. After 24 hours, apoptosis evaluation was conducted with Sub-G1 assay with hypotonic fluorochrome solution using flow cytometer.RESULTS: The average of apoptotic cells irradiated with UVB for 30 minutes was 94.2%. The 30-minutes-UVB irradiation significantly induced apoptosis in NIH3T3 cells (p=0.000). The averages of apoptotic cells by pretreatment of 0, 5, 10 and 20% CM-hUCB-MSC prior to UVB irradiation were 94.2, 46.8, 31.8 and 31.5 %, respectively. The pretreatment of 5, 10 and 20% CM-hUCB-MSC prior to UVB irradiation could significantly decrease the percentage of apoptosis caused by UVB (p=0.001).CONCLUSION: Taken together, CM-hUCB-MSC inhibited UVB-induced apoptosis in NIH3T3 cells significantly, suggesting that CM-hUCB-MSC might be a potential anti-UVB-induced apoptosis factor. Hence, further research should be explored to disclose its specific intracellular mechanism.KEYWORDS: UVB, stem cell, conditioned media, fibroblast, apoptosis, aging, secretome, NIH3T3 cell

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Proliferation and characterization of Wharton-jelly's derived mesenchymal stromal cell using plasma rich platelet and plasma platelet lysate

Cited by 4 publications

References 0 publications

Role of Herbal Extract in Stem Cell Development

Role of Herbal Extract in Stem Cell Development

Anti-cancer potency and mechanism of human umbilical cord blood stem cell

Conditioned Media of Human Umbilical Cord Blood Mesenchymal Stem Cell Inhibits Ultraviolet B-induced Apoptosis in Fibroblasts

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