Prolactin signaling in porcine adrenocortical cells: involvement of protein kinases

Kamińska, Barbara; Ciereszko, Renata E.; Opałka, Marek; Dusza, Luiza

doi:10.1016/s0739-7240(02)00173-x

Cited by 16 publications

(13 citation statements)

References 56 publications

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“…These results also suggest that hypersecretion of CRH probably occurs in hypoprolactinemia rats. Previous studies have shown that PRL could act directly on adrenal gland to drive corticosterone secretion [2,4,6,[7][8][9][10]. In the present study, PRL stimulated the secretion of corticosterone from cultured adrenal cells in synergy with low levels of ACTH in all rat models.…”

Section: Discussionsupporting

confidence: 66%

“…Several studies in the literature have examined the role of PRL on adrenal gland function as well as its effects on lactation and reproduction [1][2][3][4][5][6]. Although adrenocorticotropic hormone (ACTH) is generally considered to play a major role in the regulation of adrenocortical secretion, previous reports showed that PRL could act directly on adrenal gland to drive corticosterone secretion [2,4,6,[7][8][9][10]. Additionally, PRL receptors (PRL-R) are expressed in the adrenal cortex in several species [1,7,[11][12][13].…”

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confidence: 99%

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Physiological Roles of Prolactin in the Adrenocortical Response to Acute Restraint Stress

et al. 2007

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Abstract. The present study characterized the different hormonal responses to stress in the endocrine milieu with different circulating levels of prolactin (PRL) and examined the direct effects of PRL on adrenal steroidogenic responses to adrenocorticotropic hormone (ACTH) using experimentally induced hyperprolactinemia and hypoprolactinemia male rat models. Hyperprolactinemia was induced by transplantation of two adult female rat anterior pituitary glands under the kidney capsule for 2 weeks, and hypoprolactinemia was induced by daily subcutaneous injection of 2-Bromo-alphaErgocryptine (CB-154) for 2 weeks. Under stress conditions, the peak levels of ACTH were significantly higher in hypoprolactinemia than normal rats. Meanwhile, the peak levels of corticosterone and progesterone were significantly higher in hyperprolactinemia than in normal and hypoprolactinemia stressed rats. Results of in vitro experiments showed that adrenocortical cells in hyperprolactinemia exhibited higher basal levels of corticosterone and progesterone rats than normal and hypoprolactinemia rats. The stimulatory effect of ACTH on corticosterone and progesterone release was higher in hyperprolactinemia than hypoprolactinemia rats. In addition, PRL increased the stimulatory effect of ACTHinduced corticosterone secretion in all rat models. These results suggest that hypoprolactinemia and hyperprolactinemia rats exhibit marked differences in the response of their hypothalamic-pituitary-adrenal (HPA) axis during acute restrain stress. Additionally, these studies emphasize that the adrenal cortex might be more sensitive to ACTH stimulation in endocrine milieu with high levels of PRL resulting in high corticosterone and progesterone release.

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Section: Discussionsupporting

confidence: 66%

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confidence: 99%

Physiological Roles of Prolactin in the Adrenocortical Response to Acute Restraint Stress

et al. 2007

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“…Porcine adrenocortical cells were prepared as previously described (Kaminska et al, 2002). Briefly, adrenals were decapsulated and medullae were discarded.…”

Section: Dispersion and Incubation Of Adrenocortical Cellsmentioning

confidence: 99%

“…Cortisol and androstenedione levels were measured in the medium using specific RIAs as previously described (Kaminska et al, 2002). The specificity of antibodies against cortisol and androstenedione has been previously reported (Kaminska et al, 2000;Szafranska et al, 2002, respectively).…”

Section: Hormone Assaysmentioning

confidence: 99%

“…Studies on the effects of phytoestrogens on the adrenal cortex functions of humans and laboratory animals are not numerous and, to our knowledge, there is a lack of such studies in the pig. In our earlier paper (Kaminska et al, 2002) describing the mechanism of action of prolactin in porcine adrenocortical cells, genistein was found to inhibit cortisol secretion and, simultaneously, stimulate androstenedione release. However, in this study, from the midst of isoflavones, only genistein was used (as a protein tyrosine kinase inhibitor) at one dose only.…”

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confidence: 93%

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Phytoestrogens alter cortisol and and rostenedione secretion by porcine adrenocortical cells

Kamińska

Opałka

Dusza

2007

Acta Veterinaria Hungarica

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The effect of plant-derived, nonsteroidal estrogens (genistein, daidzein and biochanin A) on cortisol and androstenedione secretion by porcine adrenocortical cells was examined. Adrenals were harvested from locally slaughtered mature gilts on days 5-9 of the oestrous cycle. Adrenocortical cells were incubated with or without genistein, daidzein, biochanin A (0.5, 5, 10 or 50 µM), oestradiol (10, 50, 100 or 500 pg/mL) and ACTH (5 nM -positive control). All incubations were performed for 8 h (95% air and 5% CO 2 , 37 °C). ACTH increased cortisol and androstenedione secretion. Genistein, daidzein and biochanin A suppressed cortisol output, whereas androstenedione secretion was enhanced by these phytoestrogens. In contrast, oestradiol did not alter steroid secretion by porcine adrenocortical cells, which may suggest that phytoestrogens have a non-oestrogenic mechanism of action. Therefore, phytoestrogens present in commercial forage may influence adrenocortical function in pigs by decreasing cortisol and increasing androstenedione secretion. Key words: Phytoestrogens, isoflavones, adrenocortical cells, pigPhytoestrogens are phytochemicals present in whole plants, their seeds and plant-derived preserves (Reinli and Block, 1996). These compounds are widely present in legumes and beans, which are the main components of the fodder of farm animals and constitute an integral part of the human diet (Lundh, 1995;Duncan et al., 2003). Soybeans, commonly used in pig diets, are the most significant source of isoflavones (one of the phytoestrogen classes) including

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The involvement of protein kinases in signalling of opioid agonist FK 33-824 in porcine granulosa cells

Kamiński¹

2006

Animal Reproduction Science

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Prolactin signaling in porcine adrenocortical cells: involvement of protein kinases

Cited by 16 publications

References 56 publications

Physiological Roles of Prolactin in the Adrenocortical Response to Acute Restraint Stress

Physiological Roles of Prolactin in the Adrenocortical Response to Acute Restraint Stress

Phytoestrogens alter cortisol and and rostenedione secretion by porcine adrenocortical cells

The involvement of protein kinases in signalling of opioid agonist FK 33-824 in porcine granulosa cells

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