Progress in the Self-Regulation System in Legume Nodule Development-AON (Autoregulation of Nodulation)

Li, Yuhe; Pei, Yanxi; Shen, Yitong; Zhang, Rui; Kang, Mingming; Ma, Yelin; Li, Dengyao; Chen, Yuhui

doi:10.3390/ijms23126676

Cited by 12 publications

(8 citation statements)

References 125 publications

(171 reference statements)

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“…A permanent activation of Nod factor signaling by too high Nod factor concentrations could possibly impair establishment of symbiosis. Furthermore, it cannot be ruled out that high concentrations of Nod factors induce plant immunity and autoregulation responses which are known to negatively affect nodulation ( Cao et al., 2017 ; Cai et al., 2018 ; Li et al., 2022 ). The experiments shown in Figure 2 indicate that MtCHIT5b expression was stimulated by inoculation with rhizobia and by Nod factor concentrations as low as 10 -10 M. These findings indicate that the concentration of Nod factors produced by rhizobia in the rhizosphere was at least 10 -10 M. We suggest that Nod factor levels in the infection pocket are much higher.…”

Section: Discussionmentioning

confidence: 99%

The Medicago truncatula hydrolase MtCHIT5b degrades Nod factors of Sinorhizobium meliloti and cooperates with MtNFH1 to regulate the nodule symbiosis

Zhang

Fan

et al. 2022

Front. Plant Sci.

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Nod factors secreted by nitrogen-fixing rhizobia are lipo-chitooligosaccharidic signals required for establishment of the nodule symbiosis with legumes. In Medicago truncatula, the Nod factor hydrolase 1 (MtNFH1) was found to cleave Nod factors of Sinorhizobium meliloti. Here, we report that the class V chitinase MtCHIT5b of M. truncatula expressed in Escherichia coli can release lipodisaccharides from Nod factors. Analysis of M. truncatula mutant plants indicated that MtCHIT5b, together with MtNFH1, degrades S. meliloti Nod factors in the rhizosphere. MtCHIT5b expression was induced by treatment of roots with purified Nod factors or inoculation with rhizobia. MtCHIT5b with a fluorescent tag was detected in the infection pocket of root hairs. Nodulation of a MtCHIT5b knockout mutant was not significantly altered whereas overexpression of MtCHIT5b resulted in fewer nodules. Reduced nodulation was observed when MtCHIT5b and MtNFH1 were simultaneously silenced in RNA interference experiments. Overall, this study shows that nodule formation of M. truncatula is regulated by a second Nod factor cleaving hydrolase in addition to MtNFH1.

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Section: Discussionmentioning

confidence: 99%

The Medicago truncatula hydrolase MtCHIT5b degrades Nod factors of Sinorhizobium meliloti and cooperates with MtNFH1 to regulate the nodule symbiosis

Zhang

Fan

et al. 2022

Front. Plant Sci.

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“…The nodulation competence could be restored by a knock-down mutation of the CLE peptide receptor, as was shown for L. japonicus [172]. The CLE peptides are transported to the shoot through the xylem and interact with the nodule autoregulation receptor kinase (GmNARK/LjHAR1/MtSUNN/PsSYM29) [173]. Interaction of CLEs with their receptor causes inactivation of miRNA miR-2111 and, therefore, inhibition of nodulation by TML1/2 [174,175].…”

Section: Autoregulation Of Nodulationmentioning

confidence: 77%

Signaling in Legume–Rhizobia Symbiosis

Shumilina,

Soboleva,

Abakumov

et al. 2023

IJMS

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Legumes represent an important source of food protein for human nutrition and animal feed. Therefore, sustainable production of legume crops is an issue of global importance. It is well-known that legume-rhizobia symbiosis allows an increase in the productivity and resilience of legume crops. The efficiency of this mutualistic association strongly depends on precise regulation of the complex interactions between plant and rhizobia. Their molecular dialogue represents a complex multi-staged process, each step of which is critically important for the overall success of the symbiosis. In particular, understanding the details of the molecular mechanisms behind the nodule formation and functioning might give access to new legume cultivars with improved crop productivity. Therefore, here we provide a comprehensive literature overview on the dynamics of the signaling network underlying the development of the legume-rhizobia symbiosis. Thereby, we pay special attention to the new findings in the field, as well as the principal directions of the current and prospective research. For this, here we comprehensively address the principal signaling events involved in the nodule inception, development, functioning, and senescence.

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“…In contrast to WT plants, which develop rod shape nodules, the FN007 mutant plants develop round, spherical nodules. A possible explanation for this could be that the deletion of the target gene affects the expression of the leghemoglobin gene in FN007 mutant (Jiang et al, 2021), resulting in white root nodules at all stages of development, and that the expression of genes involved in root nodule development is restricted (Li et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Biological and genomic analysis of a symbiotic nitrogen fixation defective mutant in Medicago truncatula

Shen

et al. 2023

Front. Plant Sci.

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Medicago truncatula has been selected as one of the model legume species for gene functional studies. To elucidate the functions of the very large number of genes present in plant genomes, genetic mutant resources are very useful and necessary tools. Fast Neutron (FN) mutagenesis is effective in inducing deletion mutations in genomes of diverse species. Through this method, we have generated a large mutant resource in M. truncatula. This mutant resources have been used to screen for different mutant using a forward genetics methods. We have isolated and identified a large amount of symbiotic nitrogen fixation (SNF) deficiency mutants. Here, we describe the detail procedures that are being used to characterize symbiotic mutants in M. truncatula. In recent years, whole genome sequencing has been used to speed up and scale up the deletion identification in the mutant. Using this method, we have successfully isolated a SNF defective mutant FN007 and identified that it has a large segment deletion on chromosome 3. The causal deletion in the mutant was confirmed by tail PCR amplication and sequencing. Our results illustrate the utility of whole genome sequencing analysis in the characterization of FN induced deletion mutants for gene discovery and functional studies in the M. truncatula. It is expected to improve our understanding of molecular mechanisms underlying symbiotic nitrogen fixation in legume plants to a great extent.

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Progress in the Self-Regulation System in Legume Nodule Development-AON (Autoregulation of Nodulation)

Cited by 12 publications

References 125 publications

The Medicago truncatula hydrolase MtCHIT5b degrades Nod factors of Sinorhizobium meliloti and cooperates with MtNFH1 to regulate the nodule symbiosis

The Medicago truncatula hydrolase MtCHIT5b degrades Nod factors of Sinorhizobium meliloti and cooperates with MtNFH1 to regulate the nodule symbiosis

Signaling in Legume–Rhizobia Symbiosis

Biological and genomic analysis of a symbiotic nitrogen fixation defective mutant in Medicago truncatula

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