Journal of Chromatography A 2012 DOI: 10.1016/j.chroma.2012.02.071 View full text
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Pavel Jandera

Abstract: Recent advances in applications of gradient elution in two-dimensional liquid chromatography are reviewed. Benefits of simultaneous programmed elution in two-dimensional liquid chromatography are shown, with emphasis on fast gradient elution in second dimension using various gradient types and profiles. The theory of gradient elution is shortly overviewed with attention to its impacts on two-dimensional separations. Various two-dimensional gradient systems are compared in terms of peak capacity and peak produc…

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