Programmable Membrane-Mediated Attachment of Synthetic Virus-like Nanoparticles on Artificial Protocells for Enhanced Immunogenicity

Mukwaya, Vincent; Zhang, Peipei; Liu, Lingshan; Dang-i, Auphedeous Yinme; Li, Mei; Mann, Stephen; Dou, Hongjing

doi:10.1016/j.xcrp.2020.100291

Cited by 21 publications

(25 citation statements)

References 63 publications

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“…particles. Recently, particles that had been attached onto synthetic cells have been employed as signal generators and receivers to provide abundant effective mutual interaction sites, [24] as transportation channels to accurately control the inward and outward flow of substances, [25] as well as recognition sites for cancer cell targets for therapeutic applications. [21]…”

Section: Docked Decoration On Membranesmentioning

confidence: 99%

“…With regard to membranes that are decorated with synthetic polymer-based particles, a number of artificially designed properties have been introduced, such as changing the surface hydrophilicity prevent protein corona formation, [21] binding with virus-like particles on synthetic cell membrane for immune activation, [24] and inserting photothermal metal particles for energy conversion. [27] Crystallization-driven selfassembly has emerged as a promising route for the fabrication of synthetic cells with high shape anisotropy.…”

Section: Synthetic Macromolecule-based Decorationsmentioning

confidence: 99%

“…As mentioned above, the artificial cells can also be more easily programmed to display specific properties, thus offering new means to monitor, control, or complement biological functions. [24] Moreover, the short culture time, convenient operation, and fully licensed building blocks for the synthetic cells can all greatly strengthen their viability for clinical applications.…”

Section: From Synthetic Cells To Synthetic Tissuementioning

confidence: 99%

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Hierarchical Structures in Macromolecule‐Assembled Synthetic Cells

Zhou

Wang

et al. 2022

Macromol. Rapid Commun.

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Various models of synthetic cells have been developed as researchers have sought to explore the origins of life. Based on the fact that structural complexity is the foundation of higher‐order functions, this review focuses on hierarchical structures in synthetic cell models that are inspired by living systems, in which macromolecules are the dominant participants. The underlying advantages and functions provided by biomimetic higher‐order structures are discussed from four perspectives, including hierarchical structures in membranes, in the composite construction of membrane‐coated artificial cytoplasm, in organelle‐like subcellular compartments, as well as in synthetic cell–cell assembled synthetic tissues. In parallel, various feasible driving forces and approaches for the fabrication of such higher‐order structures are showcased. Furthermore, both the implemented and potential applications of biomimetic systems, bottom‐up biosynthesis, biomedical tissue engineering, and disease therapy are highlighted. This thriving field is gradually narrowing the gap between fundamental research and applied science.

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Section: Docked Decoration On Membranesmentioning

confidence: 99%

Section: Synthetic Macromolecule-based Decorationsmentioning

confidence: 99%

Section: From Synthetic Cells To Synthetic Tissuementioning

confidence: 99%

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Hierarchical Structures in Macromolecule‐Assembled Synthetic Cells

Zhou

Wang

et al. 2022

Macromol. Rapid Commun.

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“…Such basic models can encapsulate different reagents, representing minimal 'cellular' systems for cell-free studies, including those of membrane properties [8], chemically mediated communication [9,10,11], and the manipulation of genetic information [12]. State-of-art protocell materials can interact with living cells [13] for potential biotechnological applications, such as immunogenicity enhancement [14], organoid formation [15] or blood vessel vasodilation [16].…”

Section: Main Textmentioning

confidence: 99%

Building programmable multicompartment artificial cells incorporating remotely activated protein channels using microfluidics and acoustic levitation

Jin

Jamieson

Dimitriou

et al. 2022

Preprint

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Intracellular compartments are functional units that support the metabolic processes within living cells, through spatiotemporal regulation of chemical reactions and biological processes. Consequently, as a step forward in the bottom-up creation of artificial cells, building analogous intracellular architectures is essential for the expansion of cell-mimicking functionality. Herein, we report the development of a droplet laboratory platform to engineer customised complex emulsion droplets as a multicompartment artificial cell chassis, using multiphase microfluidics and acoustic levitation. Such levitated constructs provide free-standing, dynamic, definable droplet networks for the encapsulation and organisation of chemical species. Equally, they can be remotely operated with pneumatic, heating, and magnetic elements for post-processing, including the incorporation of membrane proteins; alpha-hemolysin; and large-conductance mechanosensitive channel (MscL) and their activation. The assembly of droplet networks is three-dimensionally patterned with fluidic inputs configurations determining droplet contents and connectivity. Whilst acoustic manipulation can be harnessed to reconfigure the droplet network in situ. In addition, a mechanosensitive channel, MscL, can be repeatedly activated and deactivated in the levitated artificial cell by the application of acoustic and magnetic fields to modulate membrane tension on demand. This offers possibilities beyond one-time chemically mediated activation to provide repeated, non-contact control of membrane protein function. Collectively, this will expand our capability to program and operate increasingly sophisticated artificial cells as life-like materials.

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“…Such basic models can encapsulate different reagents, representing minimal ‘cellular’ systems for cell-free studies, including those of membrane properties 9 , chemically mediated communication 10 – 12 , and the manipulation of genetic information 13 . State-of-art protocell materials can interact with living cells 14 for potential biotechnological applications, such as immunogenicity enhancement 15 , organoid formation 16 or blood vessel vasodilation 17 .…”

Section: Introductionmentioning

confidence: 99%

Building programmable multicompartment artificial cells incorporating remotely activated protein channels using microfluidics and acoustic levitation

et al. 2022

View full text Add to dashboard Cite

Intracellular compartments are functional units that support the metabolism within living cells, through spatiotemporal regulation of chemical reactions and biological processes. Consequently, as a step forward in the bottom-up creation of artificial cells, building analogous intracellular architectures is essential for the expansion of cell-mimicking functionality. Herein, we report the development of a droplet laboratory platform to engineer complex emulsion-based, multicompartment artificial cells, using microfluidics and acoustic levitation. Such levitated models provide free-standing, dynamic, definable droplet networks for the compartmentalisation of chemical species. Equally, they can be remotely operated with pneumatic, heating, and magnetic elements for post-processing, including the incorporation of membrane proteins; alpha-hemolysin; and mechanosensitive channel of large-conductance. The assembly of droplet networks is three-dimensionally patterned with fluidic input configurations determining droplet contents and connectivity, whilst acoustic manipulation can be harnessed to reconfigure the droplet network in situ. The mechanosensitive channel can be repeatedly activated and deactivated in the levitated artificial cell by the application of acoustic and magnetic fields to modulate membrane tension on demand. This offers possibilities beyond one-time chemically mediated activation to provide repeated, non-contact, control of membrane protein function. Collectively, this expands our growing capability to program and operate increasingly sophisticated artificial cells as life-like materials.

show abstract

Programmable Membrane-Mediated Attachment of Synthetic Virus-like Nanoparticles on Artificial Protocells for Enhanced Immunogenicity

Cited by 21 publications

References 63 publications

Hierarchical Structures in Macromolecule‐Assembled Synthetic Cells

Hierarchical Structures in Macromolecule‐Assembled Synthetic Cells

Building programmable multicompartment artificial cells incorporating remotely activated protein channels using microfluidics and acoustic levitation

Building programmable multicompartment artificial cells incorporating remotely activated protein channels using microfluidics and acoustic levitation

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