Prognostic Significance of p53 Protein and X-ray Repair Cross-Complementing Protein 1 in Non-Small Cell Lung Cancer

Rybárová, Silvia; Hodorová, Ingrid; Muri, Jozef; Mihálik, Jozef; Adamkov, Marián; Švajdler, Marián; D, Piovarči; Mirossay, Ladislav

doi:10.1177/030089161109700115

Cited by 7 publications

(5 citation statements)

References 31 publications

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“…In NSCLC, p53 overexpression significantly correlated with squamous cell carcinoma compared to adenocarcinoma (p = 0.008) and with tumours showing highest grade (p = 0.012). In previous studies, a statistically significant correlation between p53 overexpression and squamous cell carcinoma compared to adenocarcinoma was also reported and, in particular, Rybarova et al found a correlation between p53 overexpression and poorly differentiated tumours in NSCLC [ 15 , 16 ]. Finally, we did not find a significant correlation between positive levels of serum p53Abs and TP53 mutations in the corresponding tumours, nor between TP53 mutations and clinicopathologic features, presumably due to the small number of suitable tumour samples, while a significant correlation between TP53 mutations and tumours with the highest median rate of p53 overexpression was observed within the p53 protein-positive tumour samples (p = 0.04).…”

Section: Discussionmentioning

confidence: 96%

Prognostic role of serum p53 antibodies in lung cancer

Mattioni

Soddu²,

Prodosmo³

et al. 2015

BMC Cancer

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BackgroundMutations in the TP53 (Tumour Protein 53) gene can lead to expression of mutant p53 proteins that accumulate in cancer cells and can induce circulating p53 antibodies in cancer patients. Our aim was to evaluate the presence and prognostic role of these antibodies in lung cancer patients and to investigate whether they were related to p53 expression or TP53 mutations in tumour tissues.MethodsA total of 201 lung cancer patients were evaluated for p53 antibodies by ELISA (Enzyme-Linked Immunosorbent Assay) and control was obtained from 54 patients with non-malignant disorders; p53 expression was evaluated in 131 of the lung cancer patients by immunohistochemistry and TP53 mutations were then investigated in 53 tumours positively staining for p53 and in 12 tumours without p53 overexpression, whose DNA was available for direct sequencing.ResultsOur results show that 20.4% of cancer patients have positive levels of p53 antibodies, while none of the controls resulted positive. High levels of p53 expression are detected in 57.3% of cases and a significant correlation between serum p53 antibodies and high levels of p53 expression in the corresponding tumours is observed. In non-small cell lung cancer, p53 antibodies are significantly associated with poorly differentiated tumours; furthermore, high levels of p53 expression significantly correlated with squamous cell carcinoma and tumours with highest grade. Survival time of non-small cell lung cancer patients low/negative for serum p53 antibodies was significantly longer compared to patients with positive levels (p = 0.049); in particular, patients with squamous cell carcinoma, but not adenocarcinoma, low/negative for these antibodies show a significant better survival compared to serum-positive patients (p = 0.044).ConclusionsIn our study, detection of serum p53 antibodies in non-small cell lung cancer patients has been shown to be useful in identifying subsets of patients with poor prognosis. A significant correlation between the presence of serum p53 antibodies in lung cancer patients and p53 overexpression in the corresponding tumours was also observed. We did not find a significant correlation between levels of serum p53 antibodies and TP53 mutations in the corresponding tumours.

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Section: Discussionmentioning

confidence: 96%

Prognostic role of serum p53 antibodies in lung cancer

Mattioni

Soddu²,

Prodosmo³

et al. 2015

BMC Cancer

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“…It also inhibits DNA replication and is a check-point control molecule for progression of the cell cycle. Furthermore, p53 protein is involved in the regulation of apoptosis (53,54). We observed strong p53 nuclear staining in outer cell layer of epithelial cells of Hassall's corpuscles and subcapsular epithelial cells.…”

Section: Thymus and Markers Of Apoptosis (P53 Bcl2 And Survivin)mentioning

confidence: 68%

The normal human newborns thymus

Jablonska-Mestanova

Sisovsky²,

Danišovič³

et al. 2013

BLL

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The thymic microenvironment constitutes a unique cell environment composed of thymic epithelial cells, myoid cells, and bone marrow-derived accessory cells for the differentiation, maturation and selection of T lymphocytes. The histological feature of thymus is markedly dependent on the age of individual and on various negative stimuli. Our study group consisted of fourteen newborns whose thymuses were removed during surgery performed for various congenital heart defects. We used a palette of seven monoclonal antibodies for exact localization of different cells creating the thymic microenvironment (cytokeratin AE1/AE3, desmin, actin, S100 protein, CD68, CD20, and CD45RO) as well as three monoclonal antibodies against proteins regulating the process of apoptosis (bcl2 oncoprotein, p53 protein, and survivin). We described and microphotographically illustrated the localization of thymic cytokeratin AE1/AE3-positive epithelial cells (subcapsular part of the cortex and medulla, especially Hassall's corpuscles), dendritic cells (medulla, often inside the Hassall's corpuscles), thymic myoid cells (medulla, often in close contact with Hassall's corpuscles), macrophages (mostly cortex, but also medulla and inside the Hassall's corpuscles), B lymphocytes (thymic medulla) and CD45RO-positive T lymphocytes (mostly thymic cortex). We found p53-positive thymic epithelial cell nuclei in subcapsular part of cortex and in outer epithelial cell layer of Hassall's corpuscles (very similar to the basal layer of epidermis). Bcl2 positive lymphocytes were mostly localized in thymic medulla, especially nearby Hassall's corpuscles. The thymuses were mostly survivin-negative with exception of round cells in border between cortex and connective tissue septa (probably migrating progenitor cells) (Tab. 1, Fig. 14, Ref. 66). Full Text in PDF www.elis.sk. Acknowledgement: This study was supported by the VEGA grant agency of the Slovak Ministry of Education No. 1/0902/11 (Thymus in human ontogenesis). 66. Xing Z, Conway EM, Kang C, Winoto A. Essential role of survivin, an inhibitor of apoptosis protein, in T cell development, maturation, and homeostasis.

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“…The p53 tumor suppressor gene which shows to be the most frequent target in which their abnormalities plays a significant role in the formation of lung epithelial cell tumors (28). The p53 tumor suppressor gene is set on the human chromosome 17p13 and its product is a nuclear phosphoprotein known as p53.…”

Section: Introductionmentioning

confidence: 99%

Lemongrass Essential Oil Shows an Antiproliferative Effect on Lung Cancer Cells and Its Role in Expression of P53 Gene

Abdulhasan¹

2021

Iraqi J. Agric. Sci.

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This study was aimed to investigate the antiproliferative effect of lemongrass essential oil (LEO) from Cymbopogon citratus on A549 cell line and their effect on the expression of p53 gene in lung cancer cells. The LEO was extracted and anlaysed using GC-MS analysis, the cytotoxicity of LEO against lung cancer cells was examined using MMT assay and finally the expression of p53 was determined via qRT-PCR. The chromatogram of GC-MS exhibited 14 compounds in ELO, the major one is citral (56.4%). The cytotoxicity of LEO was dose dependent which proliferation of the lung cells were significantly inhibited with increased concentration of essential oil (P≤ 0.01) as well as the IC50 was 29.13 ppm. Furthermore, it showed that cell treated with IC50 of LEO had a significant higher gene expression (P≤ 0.05) in the fold of p53 expression than untreated cells with change fold of 4.47±0.7. In conclusion, C. citratus essential oil have been found to possess antiproliferative activity against lung cancer cells and upregulation of p53 gene which can be considered a promising treatment for lung cancer patients.

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Prognostic Significance of p53 Protein and X-ray Repair Cross-Complementing Protein 1 in Non-Small Cell Lung Cancer

Cited by 7 publications

References 31 publications

Prognostic role of serum p53 antibodies in lung cancer

Prognostic role of serum p53 antibodies in lung cancer

The normal human newborns thymus

Lemongrass Essential Oil Shows an Antiproliferative Effect on Lung Cancer Cells and Its Role in Expression of P53 Gene

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