Prognostic Significance of EIF4G1 in Patients with Pancreatic Ductal Adenocarcinoma<strong> </strong>

Abstract

Background: The advances of genomics have greatly improved the survival rate cancer patients. However, due to genetic heterogeneity, pancreatic ductal adenocarcinoma (PDAC) is still difficult to diagnose early and the survival rate is extremely low. Therefore, we identified biomarkers that predict the prognosis of PDAC patients by using independent cohort data. Methods: To develop a novel prognostic biomarker, we used gene expression and clinical data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO). In Kaplan-Meier survival curve using median values of genes as cut off, the only statistically significant gene in the three cohorts was EIF4G1. We analyzed prognostic significance of EIF4G1 using the time-dependent area under the curve (AUC) of the Uno's C-index, the AUC value of the receiver operating characteristics (ROC) at 3 years, and multivariate cox analysis. Also, we compare EIF4G1 levels between tumor and matched non-tumor. Results: EIF4G1 is the only prognostic gene patients with PDAC which was selected by Kaplan-Meier survival analysis. Kaplan-Meier survival analysis showed that high expression of EIF4G1 was associated with poor prognosis of PDAC with good discriminative ability in 3 independent cohorts. Risk stratifying ability of EIF4G1 was demonstrated by analyzing C-indices and AUC values. Multivariate cox regression analysis confirmed its prognostic significance. EIF4G1 expression was significantly higher in the PDAC tissues than in the matched normal tissues. Conclusions: Herein, the novel prognostic biomarker EIF4G1 could be used as prognostic maker for PDAC and determining suitable treatment options.

FAM213A is linked to prognostic significance in acute myeloid leukemia through regulation of oxidative stress and myelopoiesis

“…We used the following methods: Kaplan‐Meier survival curves with log‐rank test, Uno's C ‐index in the time‐dependent area under the curve (AUC) analysis, and AUC values in receiver operating characteristics (ROC) at 3 years to evaluate the accuracy of the risk stratification. These methods were performed in accordance with previously published studies . Values were obtained using the R packages “survival” and “survAUC.” In the Kaplan‐Meier analyses, we determined the optimal cutoff expression value of FAM213A that had the maximal Uno's C ‐index by fivefold cross‐validation.…”

“…These methods were performed in accordance with previously published studies. [16][17][18][19] Values were obtained using the R packages "survival" and "survAUC." In the Kaplan-Meier analyses, we determined the optimal cutoff expression value of FAM213A that had the maximal Uno's C-index by fivefold cross-validation.…”

Elevation of EIF4G1 promotes non‐small cell lung cancer progression by activating mTOR signalling

“…The formation and development of tumours are complex processes involving multiple factors, such as some key oncogenes and tumour suppressor factors 31 . In addition to promoting protein translation, EIF4G1 plays an important role in the initial stage of eukaryotic cell translation and is closely related to the occurrence and development of tumours 32,33 . Some studies have shown that the eukaryotic translation initiation factor EIF4G1 plays a positive role in the processes of tumour formation, proliferation and metastasis 19‐24 .…”

“…31 In addition to promoting protein translation, EIF4G1 plays an important role in the initial stage of eukaryotic cell translation and is closely related to the occurrence and development of tumours. 32,33 Some studies have shown that the eukaryotic translation initiation factor EIF4G1 plays a positive role in the processes of tumour formation, proliferation and metastasis. [19][20][21][22][23][24] In the present study, we found that the mRNA and protein expression levels of EIF4G1 were upregulated in NSCLC tissues, as determined by RT-PCR and immunohistochemistry analysis, and the expression levels of EIF4G1 were closely related to the differentiation of tumour cells; the higher the expression of EIF4G1 was, the poorer differentiated the tumour was.…”

Effect of Kaempferol on Cyclooxygenase 2 (Cox2) and Cytosolic Phospholipase A2 (cPLA2) Protein Expression in BALB/c Mice

“…This result is consistent with a previous study showing that serum histamine level is significantly lower in a murine allergic Rhinitis model after kaempferol administration. 23 High concentration of omega-6 administration can cause imbalance of omega-6 and omega-3 ratio. This can have negative effects on the health of mammalians.…”