Profiling the effects of microwave-assisted and soxhlet extraction techniques on the physicochemical attributes of Moringa oleifera seed oil and proteins

Nebolisa, Ngozi Maryann; Umeyor, Chukwuebuka; Ekpunobi, Uchenna Eunice; Umeyor, Immaculeta Chikamnele; Okoye, Festus Basden Chiedu

doi:10.1016/j.ocsci.2023.02.003

Cited by 8 publications

(6 citation statements)

References 51 publications

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“…The amount of desirable components that can be extracted from a specific amount of plant material is termed the plant extract yield, typically indicated as a percentage. Soxhlet extractor, a standard leaching method for over a century, was used to execute successive solid–liquid extractions to get high yields of chemicals from insoluble fractions . We obtained the extracts with different constituents based on polarity.…”

Section: Resultsmentioning

confidence: 99%

“…Soxhlet extractor, a standard leaching method for over a century, was used to execute successive solid–liquid extractions to get high yields of chemicals from insoluble fractions. 19 We obtained the extracts with different constituents based on polarity. The methanol extract (7.3%) had the highest yield, whereas the acetone and hexane extracts had comparatively lower yields ( Table 1 ).…”

Section: Resultsmentioning

confidence: 99%

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Phytochemical Screening, In Silico Molecular Docking, ADME Properties, and In Vitro Antioxidant, Anticancer, and Antidiabetic Activity of Marine Halophyte Suaeda maritima (L.) Dumort

Manojkumar,

Thandeeswaran,

Thangavel

et al. 2024

ACS Omega

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Medicinally valuable components derived from natural resources are highly desirable as prospective alternatives to synthetic drugs to treat fatal diseases, such as cancer and diabetes mellitus. Suaeda maritima (L.) Dumort (Amaranthaceae) (S. maritima) is a halophyte plant that can thrive in saline environments and possesses excellent medicinal properties. Hence, for the present investigation, S. maritima has been chosen, and its phytochemical constituents have been extracted utilizing various solvents, including hexane, acetone, and methanol, and identified by GC-MS, LC-MS, and HPLC analyses. The antioxidant activity of the compounds using DPPH, ABTS, and reducing power assays demonstrated that all three extracts of S. maritima possessed significant radical scavenging activity comparable to standard ascorbic acid with lower IC50 values (69.20–95.58 μg/mL). In addition, the evaluation of antidiabetic activity by α-amylase inhibition and α-glucosidase inhibition methods revealed that the acetone extract of S. maritima (SMAE) displayed equipotent activity of standard acarbose with an IC50 of 32.6 μg/mL. Advantageously, SMAE also exhibited better inhibition activity against the growth of lung cancer cells with an IC50 of 78.19. μg/mL and less toxicity on the noncancerous HUVEC cells with a high IC50 of 300 μg/mL. In addition, the cancer cell death mechanism via the apoptotic pathway induced by SMAE was confirmed by DAPI staining and ROS analysis. The analysis of ADME properties, including absorption, distribution, metabolism, and excretion, witnessed that the physicochemical and druglikeness factors were best catered by stigmasterol, γ-sitosterol, and vitamin E. Further, the key phytochemicals identified from SMAE were docked with CtBP1 and SOX2 bound to importin-α target proteins associated with carcinogenic pathways using Schrodinger software. The results showed that the phytochemicals, scilicet, stigmasterol, γ-sitosterol, octadecadienoic acid, and vitamin E, showed a good binding affinity with Glide scores in the range −2.845–4.018 kcal/mol. Overall, the findings support that the least investigated traditional edible medicinal mangrove-related S. maritima is high in pharmacologically active constituents and might be one of the finest sources of naturally derived molecules for drug development and delivery systems.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Phytochemical Screening, In Silico Molecular Docking, ADME Properties, and In Vitro Antioxidant, Anticancer, and Antidiabetic Activity of Marine Halophyte Suaeda maritima (L.) Dumort

Manojkumar,

Thandeeswaran,

Thangavel

et al. 2024

ACS Omega

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“…These data show that the solvent (petroleum ether) and extraction methods (MAE and SE) were suitable for the extraction of M. oleifera oil. This also indicates that petroleum ether has very good solubility for oil as well as excellent microwave irradiation properties [8].…”

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confidence: 86%

“…A 0.1 mol/L (HCl) was used for titration . A Blank titration was performed as a reference [20] [32]. The saponification value was calculated using Equation 5:…”

Section: Determination Of Saponification Valuementioning

confidence: 99%

Extraction and Comparative Evaluation on the Physicochemical Characteristics of Yemeni Moringa oleifera Seeds Oil

Al-Maqtari,

Al-Maydama,

Bahadi

et al. 2024

jast

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Moringa oleifera is a versatile tree with nutritional, medicinal and environmental benefits making it a valuable resource for communities in many regions of the world. This study aimed to extracted oil from Yemeni Moringa oleifera seeds oil (YMOSO) using two methods (cold pressing and seoxhlet n-hexane). The yields of the oils were 16.12% and 28.34, % respectively. The yields of the oils were 16.12% % and 28.34% respectively. Where it was investigate the chemical and physical characteristics by two method cold press and soxhlet n-hexane showed of Yemeni Moringa oleifera seeds oil (YMOSO), including a free fatty acid (FFA) content of 1.12 % and 2.98 %, an acid value of 5.99±0.5 mg/g and 6.52±0.5 mg/g, an iodine value of 67.68±0.1 mg/g and 67.43±0.1 mg/g, a saponification value of 189.51±0.1 mg/g and 188.49±0.1 mg/g, a refractive index of 1.46±0.01 and 1.47±0.01 at 25 °,C a moisture content of 0.24±0.03% and 0.36±0.03%, a density of 0.90±0.05 g/ml and 0.89±0.05 g/ml at 25 °C, a specific gravity of 0.968±0.03 g/ml and 0.956±0.03 g/ml at 25 °C, and a viscosity of 48 and 47 cP at 25 °C respectively. the chemical components of the oil were determined using Fourier transform infrared (FTIR) spectroscopy and Gas Chromatography-Mass Spectroscopy (GS-MS).

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“…There are two primary groups of substances that provide retinol bioactivity. One is preformed vitamin A (retinol) and the other is provitamin A carotenoids chiefly comprising beta-carotene, alpha-carotene, and cryptoxanthin [ 1 , 2 ]. As an essential fat-soluble micronutrient, vitamin A cannot be synthesized by the human body and needs to be obtained from a regular diet.…”

Section: Introductionmentioning

confidence: 99%

Contribution of Different Food Types to Vitamin A Intake in the Chinese Diet

Li,

Guo,

Zhang

et al. 2023

Nutrients

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Vitamin A is a fat-soluble micronutrient that is essential for human health. In this study, the daily vitamin A intake of Chinese residents was evaluated by investigating the vitamin A content of various foods. The results show that the dietary intake of vitamin A in common foods was 460.56 ugRAE/day, which is significantly lower than the recommended dietary reference intake of vitamin A (800 ugRAE/day for adult men and 700 ugRAE/day for adult women). Vegetables contributed the most to daily vitamin A dietary intake, accounting for 54.94% of vitamin A intake (253.03 ugRAE/day), followed by eggs, milk, aquatic products, meat, fruit, legumes, coarse cereals, and potatoes. Therefore, an increase in the vitamin A content of vegetables and the fortification of vegetable oils with vitamin A are effective ways to increase vitamin A intake to meet the recommended dietary guidelines in China. The assessment results support the design of fortified foods.

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Profiling the effects of microwave-assisted and soxhlet extraction techniques on the physicochemical attributes of Moringa oleifera seed oil and proteins

Cited by 8 publications

References 51 publications

Phytochemical Screening, In Silico Molecular Docking, ADME Properties, and In Vitro Antioxidant, Anticancer, and Antidiabetic Activity of Marine Halophyte Suaeda maritima (L.) Dumort

Phytochemical Screening, In Silico Molecular Docking, ADME Properties, and In Vitro Antioxidant, Anticancer, and Antidiabetic Activity of Marine Halophyte Suaeda maritima (L.) Dumort

Extraction and Comparative Evaluation on the Physicochemical Characteristics of Yemeni Moringa oleifera Seeds Oil

Contribution of Different Food Types to Vitamin A Intake in the Chinese Diet

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