Produksi Pigmen dan Identifikasi Kapang Penghasilnya Menggunakan Pendekatan DNA Barcoding

Sibero, Mada Triandala; Tarman, Kustiariyah; Radjasa, Ocky Karna; Sabdono, Agus; Trianto, Agus; Bachtiarini, Tiara Ulfa

doi:10.17844/jphpi.v21i1.21454

Cited by 6 publications

(6 citation statements)

References 16 publications

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“…Sibero et al stated that fungal pigment had antibacterial activity against multidrugresistant (MDR) human pathogen such as ESBL Escherichia coli, S. enterica ser. Typhi, S. haemolyticus, methicillin-resistant Staphylococcus aureus (MRSA), and Klebsiella pneumoniae [4,5,11]. Ji and Kim [18] also reported that the pigment produced by Serratia sp.…”

Section: Resultsmentioning

confidence: 99%

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Antioxidant and antimicrobial screening of an unidentified yellow pigmented marine-derived fungus

Octaverina,

Sibero,

Subagiyo

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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The exploration of fungal secondary metabolites has increased significantly since the first discovery of penicillin. In addition, pigment derived from marine fungi attracts plenty of attention due to its complex and unusual chemical structures, which have tremendous antioxidants and antimicrobial activities. Hence, our current work aimed to characterize the crude pigment extract of an unidentified fungus SUCCF0001 and its potential as an antioxidant and antimicrobial agent. The fungus was cultivated on two semi-solid media namely MEA and PDA for 14 days at 25 °C, and two different liquids media namely MEB and PDB for 14 days at 25 °C with agitation at 150 r.p.m. The semi-solid medium and the fungal mycelium from the broth culture were extracted with methanol, while the liquid medium using ethyl acetate. The crude extracts were characterized using TLC plates and then visualized using DPPH, vanillin-H2SO4, and FeCL3. The presence of carotenoid pigment was detected using TLC with β-carotene as a marker. Antioxidant bioassay was screened using the DPPH method; while antimicrobial activity bioassay was conducted using the paper disc method against Bacillus subtilis, Escherichia coli, Micrococcus luteus, Pseudomonas aeruginosa and Candida tropicalis. The results showed that only the PDB extract had radical scavenging activity (RSA) > 50% for antioxidant activity. On the other hand, antimicrobial bioassay showed that only MEB extract had antimicrobial activity against B. subtilis. TLC analysis indicated the presence of antioxidants, terpenoids and phenols in both PDB and MEB extract. However, the result of fungal pigment characterization using TLC concluded that the pigment was not a β-carotene.

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Section: Resultsmentioning

confidence: 99%

“…Afterwards, the methanol solvent was evaporated using a rotary evaporator at 40 °C, while the ethyl acetate solvent at 37 °C. Then, the crude extract was then weighed, wrapped with aluminium foil, and stored in a freezer [11].…”

Section: Secondary Metabolites Extractionmentioning

confidence: 99%

Antioxidant and antimicrobial screening of an unidentified yellow pigmented marine-derived fungus

Octaverina,

Sibero,

Subagiyo

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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“…Moreover, in this study we also applied DPPH reagent to visualize some spots with antioxidant properties. Interestingly, some potential spots which indicated by the presence of white or yellowish colour were appeared after the reagent spraying [25]. The n-hexane extract had 2 yellow spots, whereas ethyl acetate and methanol extract demonstrated 3 yellow spots.…”

Section: Resultsmentioning

confidence: 99%

“…Total Phenolic contents was expressed as mg GAE (Gallic Acid Equivalents). [25]. The pathogenic bacteria were inoculated onto Mueller-Hinton agar (MHA, HiMedia) while the fungi were revived onto Potato Dextrose Agar (PDA, HiMedia) using a streak method and incubated for 24 hours at 37 o C. On the screening day, the pathogens were suspended into Nutrient Broth (NB, Himedia) with 0,5 McFarland (HiMedia).…”

Section: Total Phenolic Content (Tpc)mentioning

confidence: 99%

Bioprospecting of katang-katang leaves (Ipomoea pes-caprae) from Sumba Island, East Nusa Tenggara: antimicrobial, antioxidant and secondary metabolites content

Nuskiya,

Sibero,

Setyati

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Ipomoea pes-caprae (Katang-katang) is one of the vegetations that grows in coastal area. Previous studies have proven that I. pes-caprae showed antibacterial, anti-inflammatory, and antioxidant properties. This research was conducted to characterize secondary metabolites content, antimicrobial activity and antioxidant activity of I. pes-caprae crude extract from Sumba, East Nusa Tenggara. The extraction was carried out using consecutive extraction method using n-hexane, ethyl acetate, and methanol with agitation (115 r.p.m for 24 hours). Phytochemical test and thin layer chromatography were performed to analyse the secondary metabolites in I. pes-caprae. In addition, total phenolic content (TPC) was calculated using Folin-Ciocalteu method. The antimicrobial activity was conducted using paper disc diffusion method against Bacillus cereus, Salmonella typhi, Escherichia coli, Micrococcus luteus, Candida albicans, and C. tropicalis. Phytochemical test and TLC analysis showed that n-hexane extract contained flavonoid, tannin, and steroid; ethyl acetate extract contained alkaloid, flavonoid, and terpenoid; while methanol extract contained alkaloid, tannin, and steroid. The antimicrobial activity gave negative results against all microbial pathogen. The ethyl acetate and methanol extracts were noted as a strong antioxidant agent with IC50 values of 65.7 ppm and 81.8 ppm. Moreover, TPC in each extract was 3.7 mg GAE/g in n-hexane extract, 6.4 mg GAE/g in ethyl acetate extract and 8.9 mg GAE/g in methanol extract.

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“…ITS 1 primary as forward and ITS 4 as reverse was chosen because the primary was in its conservative loci region for DNA amplification from fungi (Sibero et al, 2018b). The location of the primary conservative areas of ITS 1 and ITS 4 can be seen in Figure 5.…”

Section: Activity Of Proteolyticmentioning

confidence: 99%

Molecular Identification and Biotechnological Potential of Cerithidea cingulata-and Lottia scabra Associated Fungi as Extracellular Enzyme Producer and Anti-Vibriosis Agent

Trianto,

Radjasa,

Subagiyo

et al. 2023

Indo. J. Mar. Sci.

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Mangroves are complex, unique, and dynamic ecosystems that host organisms that have special physiological adaptations to fluctuations in salinity, temperature, and pH. Gastropods have an important role in the mangrove ecosystem for food cycles and webs by helping to provide nutrients and micro-habitats for microbes. Micro-fungi isolated from mangrove ecosystems are productive sources of enzymes and bioactive compounds such as antibacterial and antifungal. In this study a explored the enzymatic and anti-vibrio activity of fungi associated with the marine gastropod Cerithidea cingulata and Lottia scabra. collected in three mangrove forests in North Sulawesi, Indonesia. The fungi associated with the specimens were inoculated on malt extract agar (MEA) using the tapping method. Enzymatic and anti-vibrio assays were conducted applying the plug method. Positive effects of the fungal compounds on Vibrio were indicated by clear zones formed around the isolates. A total of 13 fungal species were isolated from C. cingulata (19MB-C5 and 19MBa-A4) and L. scabra (19MT-07 and 19MT-18). Most fungal isolates have moderate growth rate. The isolate with highest cellulolytic, proteolytic, and anti-vibriosis activity (Vibrio parahaemolyticus, V. harveyi, V. vulnificus, and V. alginolyticus), derived from C. cingulata 19MB-C5 was closely related to Aspergillus niger, as revealed by molecular analysis using the universal primers ITS1 and ITS4 (similarity value 99%). A. niger 19MB-C5-3 has potential as a bioremediation and anti-vibrio agent that can be applied in aquaculture.

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Produksi Pigmen dan Identifikasi Kapang Penghasilnya Menggunakan Pendekatan DNA Barcoding

Cited by 6 publications

References 16 publications

Antioxidant and antimicrobial screening of an unidentified yellow pigmented marine-derived fungus

Antioxidant and antimicrobial screening of an unidentified yellow pigmented marine-derived fungus

Bioprospecting of katang-katang leaves (Ipomoea pes-caprae) from Sumba Island, East Nusa Tenggara: antimicrobial, antioxidant and secondary metabolites content

Molecular Identification and Biotechnological Potential of Cerithidea cingulata-and Lottia scabra Associated Fungi as Extracellular Enzyme Producer and Anti-Vibriosis Agent

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