Low-severity canopy disturbance presumably influences forest carbon dynamics during the course of stand development, yet the topic has received relatively little attention. This is surprising because of the frequent occurrence of such events and the potential for both the severity and frequency of disturbances to increase as a result of climate change. We investigated the impacts of low-severity canopy disturbance and average insect defoliation on forest carbon stocks and rates of carbon sequestration in mature aspen mixedwood forests of varying stand age (ranging from 61 to 85 years), overstory composition, stocking level, and site quality. Stocking level and site quality positively affected the average annual aboveground tree carbon increment (C AAI ), while stocking level, site quality, and stand age positively affected tree carbon stocks (C TREE ) and total ecosystem carbon stocks (C TOTAL ). Cumulative canopy disturbance (DIST) was reconstructed using dendroecological methods over a 29-year period. DIST was negatively and significantly related to soil carbon (C SOIL ), and it was negatively, albeit marginally, related to C TOTAL . Minima in the annual aboveground carbon increment of trees (C AI ) occurred at sites during defoliation of aspen (Populus tremuloides Michx.) by forest tent caterpillar (Malacosoma disstria Hubner), and minima were more extreme at sites dominated by trembling aspen than sites mixed with conifers. At sites defoliated by forest tent caterpillar in the early 2000s, increased sequestration by the softwood component (Abies balsamea (L.) Mill. and Picea glauca (Moench) Voss) compensated for overall decreases in C AI by 17% on average. These results underscore the importance of accounting for low-severity canopy disturbance events when developing regional forest carbon models and argue for the restoration and maintenance of historically important conifer species within aspen mixedwoods to enhance stand-level resilience to disturbance agents and maintain site-level carbon stocks.Résumé : Les perturbations peu sévères du couvert forestier influencent probablement la dynamique du carbone forestier au cours du développement d'un peuplement bien que ce sujet ait été relativement peu étudié. C'est surprenant parce que de tels événements se produisent souvent et que la fréquence et la gravité de ces perturbations pourraient augmenter à cause des changements climatiques. Nous avons étudié les impacts d'une perturbation peu sévère du couvert forestier et d'une défoliation périodique par les insectes sur les stocks de carbone forestier et le taux de piégeage du carbone dans des peuplements mixtes de peuplier mature qui variaient en termes d'âge (de 61 à 85 ans), de composition du couvert forestier, de densité et de qualité de station. La densité du peuplement et la qualité de station ont influencé positivement l'accroissement annuel moyen du carbone aérien des arbres (C AAM ) alors que la densité et l'âge du peuplement ainsi que la qualité de station ont influencé positivement le stock de carbone d...