Productive Infection and Cell-Free Transmission of Human T-Cell Leukemia Virus in a Nonlymphoid Cell Line

Clapham, Paul R.; Nagy, K.; Cheingsong-Popov, Rachanee; Exley, Mark A.; Weiss, Robin A.

doi:10.1126/science.6316502

Cited by 205 publications

(87 citation statements)

References 19 publications

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“…Retroviruses may induce cell fusion without the occurrence of viral replication (38). Indeed, most mammalian cell lines susceptible to fusion when cultured with C91/PL are not permissive for viral replication (27). We therefore sought to determine whether HTLV-I is capable of penetrating and replicating within endothelial cells by detecting their capacity to produce viral antigens.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Infection of human endothelial cells by human T-cell leukemia virus type I.

Hoxie¹,

Matthews²,

Cines³

1984

Proc. Natl. Acad. Sci. U.S.A.

130

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The effects of the human T-cell leukemia virus type I (HTLV-I) on cultured human endothelial cells were evaluated. Coculture of endothelial monolayers with either irradiated HTLV-producing lymphocytes or cell-free virus resulted in the production of multinucleated syncytia. The development of syncytia was inhibited by sera from patients with adult T-cell leukemia/lymphoma (ATLL). HTLV antigens were present on endothelial syncytia passaged in culture for >3 months as detected by an anti-p19 monoclonal antibody, which detects a core protein of HTLV-I, and by ATLL sera. Moreover, these HTLV-infected endothelial cells were then able to infect and transform normal cord blood T lymphocytes with HTLV. These studies demonstrate that human endothelial cells are susceptible to productive HTLV-I infection in vitro and may have relevance for the spectrum of human disease associated with this family of retroviruses.

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Section: Resultsmentioning

confidence: 99%

“…Moreover, viral pseudotypes bearing HTLV-I or HTLV-II envelope glycoproteins can penetrate a variety of mammalian cell lines (24). In several of these susceptible cells, syncytia were induced by cocultivation with HTLV-I-producing cells (25,26) and in a single human osteosarcoma cell line stable HTLV-I replication could be demonstrated (27).…”

mentioning

confidence: 99%

Infection of human endothelial cells by human T-cell leukemia virus type I.

Hoxie¹,

Matthews²,

Cines³

1984

Proc. Natl. Acad. Sci. U.S.A.

130

View full text Add to dashboard Cite

show abstract

“…In vitro and in vivo tropism of HTLV-1 Studies documenting in vitro infection and dissemination of HTLV-1 to non-T cells, including nonlymphoid cells, were published soon after the identification of HTLV (Yamamoto et al, 1982b;Clapham et al, 1983;Hayami et al, 1984). Productive transmission of natural HTLV-1 isolates to primary human endothelial cell cultures (Ho et al, 1984;Hoxie et al, 1984), monocyte and microglial cells (Hoffman et al, 1992), as well as basal mammary epithelial cells (LeVasseur et al, 1998) has been reported.…”

Section: Htlv-1 Tropism: In Vitro or In Vivo Veritas?mentioning

confidence: 99%

HTLV-1 tropism and envelope receptor

et al. 2005

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The identification of CD4 as the primary receptor for HIV followed shortly after the discovery of the virus, but the HTLV receptor remained long elusive, until its recent identification as the GLUT1 glucose transporter. In the present review, we describe the status of the literature that surrounded this discovery as well as the in vitro and in vivo observations that led to the identification of GLUT1. Also, we will explore a few tracks to conciliate the in vitro and in vivo data on HTLV-1 tropism within the context of the HTLV literature that has accumulated over the past 25 years. A close examination of these data leads us to conclude that the preferential detection of HTLV-1 in CD4 þ T lymphocyte subsets in vivo, even in the absence of leukemia, is not likely to be directly related to envelope receptor interactions, but rather to an array of postentry selection bottlenecks in vivo. Furthermore, we propose that infection of other hematopoietic and nonhematopoietic cells is likely to take place during the lifetime of an individual, with a burst early during the infection. Oncogene (2005) 24, 6016-6025.

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“…As discussed more thoroughly in the following section, ATLV/HTLV can infect not only human lymphocytes but also non-human cells. Clapham et al (1983) obtained a HTLV-positive cell line, HOS/PL, by the co-culture of the human osteogenic sarcoma line HOS with the HTLV-carrying C91/PL cell line. Cell-free virus derived from this (unpublished).…”

Section: Cell-lree Viral Ir!ti'ctionmentioning

confidence: 99%