Production of wheat-rye substitution lines and identification of chromosome composition of karyotypes using C-banding, GISH, and SSR markers

Silkova, O. G.; Dobrovolskaya, O. B.; Dubovets, N. I.; Адонина, И. Г.; Kravtsova, L. A.; Roeder, M. S.; Салина, Е. А.; Shchapova, A. I.; Shumny, V. K.

doi:10.1134/s1022795406060093

Cited by 41 publications

(22 citation statements)

References 21 publications

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“…Four rye mapping populations previously used for mapping over 200 RFLP, SSR, and SNP loci Khlestkina et al 2004;Varshney et al 2007) were taken for genetic mapping of some of the cloned genes. The set of 'Chinese Spring'-'Imperial' addition lines (Driscoll and Sears 1971) and wheat-rye chromosome substitution line 'L 2R(2D)' (carrying S. cereale 'Onokhoiskaya' chromosome 2R on genotypic background of two wheat cultivars 'Saratovskaya 29' and 'Novosibirskaya 67'; Silkova et al 2006) were used in gene expression analysis by RT-PCR. DNA was available from the previous studies Khlestkina et al 2004;Silkova et al 2006).…”

Section: Methodsmentioning

confidence: 99%

“…The set of 'Chinese Spring'-'Imperial' addition lines (Driscoll and Sears 1971) and wheat-rye chromosome substitution line 'L 2R(2D)' (carrying S. cereale 'Onokhoiskaya' chromosome 2R on genotypic background of two wheat cultivars 'Saratovskaya 29' and 'Novosibirskaya 67'; Silkova et al 2006) were used in gene expression analysis by RT-PCR. DNA was available from the previous studies Khlestkina et al 2004;Silkova et al 2006). Phenotyping for anthocyanin coloration and/or RNA extraction was performed, when seedlings were grown on Wlter paper at 16°C under continuous light.…”

Section: Methodsmentioning

confidence: 99%

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Anthocyanin biosynthesis genes location and expression in wheat–rye hybrids

2009

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Studies into gene expression in a foreign background contribute toward understanding of how genes derived from different species or genera manages to co-exist in a common nucleus, on the one hand, and help to estimate possible effectiveness of wide hybridization for cultivated plant improvement, on the other hand. The aim of this study was to investigate conservation of wheat and rye expression networks, using the anthocyanin biosynthesis pathway (ABP) genes as a model system. We isolated and analyzed ABP genes encoding enzymes acting at different steps of the pathway: chalcone-flavanone isomerase (CHI), flavanone 3-hydroxylase (F3H), anthocyanidin synthase (ANS), and anthocyanidin-3-glucoside rhamnosyltransferase (3RT). The rye ABP genes locations we determined (Chi on chromosome 5RL, F3h on 2RL, Ans on 6RL, 3Rt on 5RL, the regulatory Rc--red coleoptile--gene on 4RL) were in agreement with the rearrangements established between rye and wheat chromosomes. Expression of the ABP structural genes was studied in wheat-rye chromosome addition and substitution lines. F3h activation by the Rc gene was found to be critical for the red coleoptile trait formation. It was shown that the rye regulatory Rc gene can activate the wheat target gene F3h and vice versa wheat Rc induces expression of rye F3h. However, lower level of expression of rye F3h in comparison with that of the two wheat orthologues in the wheat-rye chromosome substitution line 2R(2D) was observed. Thus, although work of the wheat and rye ABP gene systems following the formation of wheat-rye hybrids is finely coordinated, some divergence exists between rye and wheat ABP genes, affecting level of gene expression.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Anthocyanin biosynthesis genes location and expression in wheat–rye hybrids

2009

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“…cereale L. cv. Vyatka-1Rv(1A) (Silkova et al 2006(Silkova et al , 2007b. In these lines, a homologous pair of breadwheat chromosomes is replaced by a homeologous pair of rye chromosomes.…”

Section: Plant Materialsmentioning

confidence: 99%

“…Chromosome behaviour in the meiocytes of androgenic haploids of line 2R(2D) 1 (Silkova et al 2009) was similar to that of the hybrids between 2R(2D) 1 and rye. To further clarify the specific features of meiosis in amphihaploids, we used the following wheat-rye disomic substitution lines (2n = 42): 1R(1A), 1Rv(1A), 2R(2D) 1 , 2R(2D) 2 , 2R(2D) 3 , 5R(5D), 5R(5A), and 6R(6A) (Silkova et al 2006(Silkova et al , 2007b. Our goal was to analyze the genotype-specific effects of these lines on the regulation of meiotic division in wheat-rye amphihaploids by comparing male gametogenesis and seed set in hybrids of wheat cultivar Saratovskaya 29 and its single chromosome substitution lines crossed with the rye cv.…”

Section: Introductionmentioning

confidence: 99%

Patterns of meiosis in ABDR amphihaploids depend on the specific type of univalent chromosome division

2010

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Meiosis in wide F 1 hybrids in the tribe Triticeae is complex and irregular. Despite extensive research, the meiotic pathways in such hybrids remain largely unknown. In this study, the effect of the genotypes of disomic wheat-rye substitution lines on the regulation of meiosis in ABDR amphihaploids was analysed by comparing microsporogenesis and seed set under self pollination in hybrids of normal bread wheat (Triticum aestivum L.) and wheats with single chromosome substitutions 1R(1A), 1Rv(1A),

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“…Although generating a segregated population from wheat and synthetic allohexaploids usually involves backcrossing of F 1 plants to one of the parents, few attempts were reported for the production of segregating population without backcrossing (Hassani et al 2003) because the F 1 plants are highly sterile. Cytological markers such as C-banding, fluorescence in situ hybridization (FISH), genomic in situ hybridization (GISH) and molecular markers can be used in chromosome identification and screening addition, substitution and recombinant lines (Mullan et al 2005, Silkova et al 2006, Qi et al 2007, Anugrahwati et al 2008, Hřibová 2008, Landjeva et al 2008. GISH is among the most powerful techniques to identify alien chromatin in the wheat background.…”

Section: Introductionmentioning

confidence: 99%

Cytogenetic analysis of hybrids derived from wheat and Tritipyrum using conventional staining and genomic in situ hybridization

Mirzaghaderi¹,

Karimzadeh²,

Hassani³

et al. 2010

Biol. plant.

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The new salt tolerant cereal, Tritipyrum (2n=6x=42, AABBE b E b ) offers potential to introduce desirable characters for wheat improvements. This study was aimed to generate a segregating population from Iranian local wheat cultivars (2n=6x=42, AABBDD) and Tritipyrum crosses, study of the meiotic behaviour in F 2 hybrids and identification of E b chromosomes in F 3 individuals. Results showed meiotic abnormalities in F 2 plants and different pairing frequency in the meiosis among F 2 plants. Genomic in situ hybridization revealed that total and E b chromosome number of F 3 seeds ranged from 39 to 45 and 0 to 10, respectively. A significant prevalence of hyper-aneuploidy was observed among F 3 genotypes. C-banding patterns identified E b chromosomes in Tritipyrum, indicating that it also can be useful to study wheat-Tritipyrum derivatives.

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Production of wheat-rye substitution lines and identification of chromosome composition of karyotypes using C-banding, GISH, and SSR markers

Cited by 41 publications

References 21 publications

Anthocyanin biosynthesis genes location and expression in wheat–rye hybrids

Anthocyanin biosynthesis genes location and expression in wheat–rye hybrids

Patterns of meiosis in ABDR amphihaploids depend on the specific type of univalent chromosome division

Cytogenetic analysis of hybrids derived from wheat and Tritipyrum using conventional staining and genomic in situ hybridization

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