Production of transgenic chickens from purified primordial germ cells infected with a lentiviral vector

Motono, Makoto; Yamada, Yuki; Hattori, Yukio; Nakagawa, Ryo; Nishijima, Ken‐ichi; Iijima, Shinji

doi:10.1016/j.jbiosc.2009.10.007

Cited by 43 publications

(54 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although resolving the exact molecular mechanism that underlies this observation will require additional studies, the heterogeneity of chFP expression in Tg(PGK1:H2B-chFP) quail should help us better understand how cell proliferation rates contribute to the differential growth of various tissues and regions during early embryogenesis (Ridenour et al, 2012;SakaueSawano et al, 2008). The Tg(PGK1:H2B-chFP) quail adds to an ever-growing list of transgenic lines generated over the past decade in quail (Sato and Lansford, 2013;Sato et al, 2010;Scott and Lois, 2005;Seidl et al, 2013), chicken (Balic et al, 2014;Chapman et al, 2005;McGrew et al, 2004;Motono et al, 2010;Rozbicki et al, 2015) and songbird (Agate et al, 2009) that express reporter proteins in a ubiquitous or tissue-specific manner.…”

Section: Tg(pgk1:h2b-chfp) Acts As a Dynamic Reporter Of Cell Prolifementioning

confidence: 99%

“…Transgenic avians that express fluorescent reporters constitutively, or in a cell-specific manner, allow for the direct imaging of a large number of cellular behaviors including tissue assembly and differentiation. Over recent years a number of transgenic chicken lines have been produced using eGFP as the marker for the transgene driven by strong ubiquitous promoters such as CMV, RSV, PGK and CAG (McGrew et al, 2004(McGrew et al, , 2008Chapman et al, 2005;Koo et al, 2004;Motono et al, 2010;Macdonald et al, 2012;Park and Han, 2012). In addition, transgenic quail lines using cell-specific promoters have allowed the dynamic imaging of particular tissue types, including endothelial cells (Sato et al, 2010) and neurons (Scott and Lois, 2005;Seidl et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Transgenic quail to dynamically image amniote embryogenesis

et al. 2015

View full text Add to dashboard Cite

Embryogenesis is the coordinated assembly of tissues during morphogenesis through changes in individual cell behaviors and collective cell movements. Dynamic imaging, combined with quantitative analysis, is ideal for investigating fundamental questions in developmental biology involving cellular differentiation, growth control and morphogenesis. However, a reliable amniote model system that is amenable to the rigors of extended, high-resolution imaging and cell tracking has been lacking. To address this shortcoming, we produced a novel transgenic quail that ubiquitously expresses nuclear localized monomer cherry fluorescent protein (chFP). We characterize the expression pattern of chFP and provide concrete examples of how Tg(PGK1:H2B-chFP) quail can be used to dynamically image and analyze key morphogenetic events during embryonic stages X to 11.

show abstract

Section: Tg(pgk1:h2b-chfp) Acts As a Dynamic Reporter Of Cell Prolifementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Transgenic quail to dynamically image amniote embryogenesis

et al. 2015

View full text Add to dashboard Cite

show abstract

“…The HIV-1-based lentiviral vector plasmid pSicoR which expresses eGFP by CMV promoter (Addgene plasmid 11579) (Ventura et al 2004) was obtained from Addgene (Cambridge, MA, USA). The lentiviral vector pLSi/DAeGFP expressing eGFP under the control of a chicken actin promoter has been described previously (Motono et al 2010). pLSi/CMVST6/ DAeGFP was constructed by inserting the expression cassette of chicken sialyltransferase 6 controlled by a CMV promoter into pLSi/DAeGFP.…”

Section: Plasmid Constructionmentioning

confidence: 99%

“…Lentiviral vectors were produced by transfecting vectors pLP1 and 2, pVSV-G, and pLSi/CMVST6/DAeGFP or pLSi/OVA-TREhEPO-IRES-tTA/CMVeGFP-W as reported previously (Motono et al 2010). The viral titer was determined by infecting Hela cells based on eGFP expression.…”

Section: Evaluation Of Transfection Efficiency and Productivity Of Lementioning

confidence: 99%

Enhanced lentiviral vector production in 293FT cells expressing Siglec-9

et al. 2014

Self Cite

View full text Add to dashboard Cite

Siglecs, sialic acid-recognizing Ig-superfamily lectins, regulate various aspects of immune responses, and have also been shown to induce the endocytosis of binding materials such as anti-Siglec antibodies or sialic acid-harboring bacteria. In this study, we demonstrated that the expression of Siglec-9 enhanced the transfection efficiency of several cell lines such as macrophage RAW264 and non-hematopoietic 293FT cells. We applied this finding to the production of a lentiviral vector in which cells were transfected simultaneously with multiple vectors, and achieved a twice increase in viral production levels. Furthermore, 293FT cells expressing lectin-defective Siglec-9 produced threeto seven-fold higher titer of viral vector compared with parental 293FT cells. These results suggest that Siglec-9 enhanced lentiviral vector production in a lectin-independent manner.

show abstract

“…1,2) Several methods have been developed to produce transgenic chickens. [3][4][5][6][7][8][9][10] Usually, viral vectors are injected into embryos or introduced into PGCs, which are the precursors of eggs and sperm. Retroviral and lentiviral vectors are used for exogenous gene delivery, but the size of the transgenes that can be delivered is limited by viral packaging capacity.…”

mentioning

confidence: 99%

Improvement of Transfection Efficiency in Cultured Chicken Primordial Germ Cells by Percoll Density Gradient Centrifugation

Oishi

2010

Bioscience, Biotechnology, and Biochemistry

View full text Add to dashboard Cite

Chicken primordial germ cells (PGCs) differentiate into germ cells in gonads. Because PGCs can be cloned and cultured maintaining germline competency, they are a good means of modifing the chicken genome, but the efficiency of plasmid transfection into PGCs is very low. In this study, I attempted to improve the efficiency of PGC transfection. Cultured PGCs were purified by Percoll density gradient centrifugation, and were then transfected with plasmid DNA. For transient transfection, the transfection efficiency increased more than 7-fold by the Percoll method. The efficiency of stable transfection of PGCs also increased significantly. The stable transfectants that were isolated by this method accumulated in the developing gonads after microinjection into bloodstream of chick embryos, indicating that gene transfection by Percoll purification did not alter the function of PGCs in vivo.

show abstract

Production of transgenic chickens from purified primordial germ cells infected with a lentiviral vector

Cited by 43 publications

References 37 publications

Transgenic quail to dynamically image amniote embryogenesis

Transgenic quail to dynamically image amniote embryogenesis

Enhanced lentiviral vector production in 293FT cells expressing Siglec-9

Improvement of Transfection Efficiency in Cultured Chicken Primordial Germ Cells by Percoll Density Gradient Centrifugation

Contact Info

Product

Resources

About