Production of serum antibodies that recognise epitopes located on the R3 region of Escherichia coli core lipopolysaccharides by patients infected with strains of enterohaemorrhagic E. coli

Chart, Henrik; Cheasty, T.; Willshaw, G. A.

doi:10.1099/0022-1317-51-12-1050

Cited by 7 publications

(20 citation statements)

References 29 publications

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“…5 Some patients infected with EHEC strains may produce antibodies reactive with the R3-core of the LPS molecule that is present in the majority of EHEC and other E. coli strains. 27,28 The cross-reactivity between the various LPS was evidenced by the absorption assays, even in the absorption with LPS O111 and LPS O26 that were selected due to the presence of the R3-core in these LPS. 27 The reductions in anti-O55 and anti-O157 LPS IgM levels may be attributed to the absorption of antibodies reactive to common epitopes of these LPS.…”

Section: Discussionmentioning

confidence: 99%

Acquisition of Serum Antibodies Reactive With Enterohemorrhagic Escherichia coli Virulence-Associated Factors by Healthy Brazilian Children and Adults

Palmeira¹,

Carbonare²,

Guth³

et al. 2009

Pediatric Infectious Disease Journal

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Section: Discussionmentioning

confidence: 99%

Acquisition of Serum Antibodies Reactive With Enterohemorrhagic Escherichia coli Virulence-Associated Factors by Healthy Brazilian Children and Adults

Palmeira¹,

Carbonare²,

Guth³

et al. 2009

Pediatric Infectious Disease Journal

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“…The J5 LPS has been widely used in immunochemical studies, in analyses of endotoxicity, and in attempts to develop antibodies against core OS-lipid A that would be broadly cross-reactive and protective in applications such as treatment of sepsis (15)(16)(17)(18)(19). Antibodies cross-reacting with R3 core OS epitopes were detected in 6 of 10 patients infected with different EHEC strains (20), and it has been proposed that IgG and IgA responses to the R3 core OS may be protective against E. coli and Shigella spp. (21).…”

Section: Lipopolysaccharide (Lps)mentioning

confidence: 99%

Chromosomal and Plasmid-encoded Enzymes Are Required for Assembly of the R3-type Core Oligosaccharide in the Lipopolysaccharide of Escherichia coli O157:H7

Kaniuk

Vinogradov

et al. 2004

Journal of Biological Chemistry

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The type R3 core oligosaccharide predominates in the lipopolysaccharides from enterohemorrhagic Escherichia coli isolates including O157:H7. The R3 core biosynthesis (waa) genetic locus contains two genes, waaD and waaJ, that are predicted to encode glycosyltransferases involved in completion of the outer core. Through determination of the structures of the lipopolysaccharide core in precise mutants and biochemical analyses of enzyme activities, WaaJ was shown to be a UDP-glucose:(galactosyl) lipopolysaccharide ␣-1,2-glucosyltransferase, and WaaD was shown to be a UDP-glucose:(glucosyl)lipopolysaccharide ␣-1,2-glucosyltransferase. The residue added by WaaJ was identified as the ligation site for O polysaccharide, and this was confirmed by determination of the structure of the linkage region in serotype O157 lipopolysaccharide. The initial O157 repeat unit begins with an N-acetylgalactosamine residue in a ␤-anomeric configuration, whereas the biological repeat unit for O157 contains ␣-linked N-acetylgalactosamine residues. With the characterization of WaaJ and WaaD, the activities of all of the enzymes encoded by the R3 waa locus are either known or predicted from homology data with a high level of confidence. However, when core oligosaccharide structure is considered, the origin of an additional ␣-1,3-linked N-acetylglucosamine residue in the outer core is unknown. The gene responsible for a nonstoichiometric ␣-1,7-linked N-acetylglucosamine substituent in the heptose (inner core) region was identified on the large virulence plasmids of E. coli O157 and Shigella flexneri serotype 2a. This is the first plasmid-encoded core oligosaccharide biosynthesis enzyme reported in E. coli.

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“…(2000). Thirty‐three of the strains used in this study had been used previously to investigate human antibody binding to the core‐LPS (Chart et al. 2002).…”

Section: Discussionmentioning

confidence: 99%

“…2001). A study by Chart et al. (2002) used human serum antibodies, from patients infected with strains of EHEC, to demonstrate that routine serodiagnosis of infections with O157 VTEC was not influenced by antibodies to the R3‐core.…”

Section: Introductionmentioning

confidence: 99%

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The expression of an R3 lipopolysaccharide-core by pathotypes of Escherichia coli

Chart¹,

Perry²,

Jenkins³

2004

J Appl Microbiol

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Aims: To investigate the incidence of an R3 lipopolysaccharide (LPS)-core amplicon in a range of pathotypes of Escherichia coli, including Verocytotoxin-producing E. coli (VTEC), enteroaggregative E. coli (EAggEC) and enteropathogenic E. coli (EPEC). Methods and Results: A total of 100 strains of E. coli belonging to a range of pathotypes, including 41 strains of VTEC, were screened for the genes encoding the R3 LPS-core using PCR. Fifty-four per cent produced an amplicon with the R3 primer set. Of the 41 VTEC, 66% had an R3 LPS-core with a PCR product being observed with all strains belonging to serotypes O26:H11, O111ac:H-and O145:H25. However, 46% of enteroaggregative E. coli and 50% of enteropathogenic E. coli were also shown to have an R3 LPS-core structure. Conclusions: Strains with an R3 LPS-core are widely distributed within the species E. coli. Significance and Impact of the Study: Strains of E. coli with an R3 LPS-core structure appear not to be associated with a specific pathotype.

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Production of serum antibodies that recognise epitopes located on the R3 region of Escherichia coli core lipopolysaccharides by patients infected with strains of enterohaemorrhagic E. coli

Cited by 7 publications

References 29 publications

Acquisition of Serum Antibodies Reactive With Enterohemorrhagic Escherichia coli Virulence-Associated Factors by Healthy Brazilian Children and Adults

Acquisition of Serum Antibodies Reactive With Enterohemorrhagic Escherichia coli Virulence-Associated Factors by Healthy Brazilian Children and Adults

Chromosomal and Plasmid-encoded Enzymes Are Required for Assembly of the R3-type Core Oligosaccharide in the Lipopolysaccharide of Escherichia coli O157:H7

The expression of an R3 lipopolysaccharide-core by pathotypes of Escherichia coli

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