Production of Semliki Forest Virus from hamster kidney cells in deep culture vessels

Telling, R. C.; Radlett, P. J.; Mowat, G. N.

doi:10.1002/bit.260090210

Cited by 9 publications

(2 citation statements)

References 7 publications

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“…could be used to seed a 400 1. culture vessel without a reduction in the titre of peak virus. It is of interest that Telling, Radlett & Mowat (1967), using the same cell system, found that Semliki Forest virus yield increased as the input virus decreased.…”

Section: Discussionmentioning

confidence: 99%

Factors affecting the production of foot-and-mouth disease virus in deep suspension cultures of BHK21 Clone 13 cells

Capstick

Garland

Chapman

et al. 1967

J. Hyg.

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For maximum utilization of deep cultures to produce FMD virus it was important to have adequate control of culture temperature and pH. Culture temperature should be controlled within the range 34·25°7–35°C. and culture pH at 7·2. The culture system became less efficient as the cell concentration was increased from 1 × 106·0 to 9 × 106·0 cells/ml. A cell concentration of 2·5 × 106·0 cells/ml. represented a working compromise between efficiency and antigen titre/ml. for inactivated FMD vaccine production.The input virus/cell ratio had no effect on the time or titre of peak virus yield in the range 1:1 to 1:320. This makes the production of seed virus from small numbers of monolayer cultures feasible and economical.Virus yield was improved by the addition of 5% serum. It would be more satisfactory if a serum-free cell strain could be developed.

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Section: Discussionmentioning

confidence: 99%

Factors affecting the production of foot-and-mouth disease virus in deep suspension cultures of BHK21 Clone 13 cells

Capstick

Garland

Chapman

et al. 1967

J. Hyg.

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“…The cap has been drilled and tapped to accept a bulkhead quickconnect [S] and two * in. pipe plugs [9]. These plugs have beon drilled through (+ in.)…”

Section: Spinner Flaskmentioning

confidence: 99%

Design of a large‐scale mammalian cell, suspension culture facility

Lynn¹,

Acton²

1975

Biotech & Bioengineering

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SummaryThe design of a suspension culture facility capable of producing approximately lO1f cells'per week has been developed on a small-scale system which has evolved from various architectural, engineering, biological, and biohazard considerations.The smaller system is composed of spinner flasks (50 ml to 8 liters) modified for semicontinuous culture conditions, metal reservoirs, a continuous flow centrifuge, and supportive equipment. The large system which is under construction is composed of metallic vessels of up to 500 liter working volume with hard plumbing, monitors, controllers, recorders, continuous flow centrifuge and other ancillary equipment. This system begins with medium preparation and ends with harvesting of cells and disposition of supernatant. The design of this turn-key operation was developed over a two and one-half year period through the cooperation of private industry, the federal government, and the academic community.

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Homogeneous cultivation of animal cells for the production of virus and virus products

Hemert

Kilburn

Wezel

1969

Biotech & Bioengineering

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SummaryHomogeneous technique facilitates the cultivation of large quantities of cells, reduces the risk of contamination by eliminating many manipulations, and makes practical the control of conditions such as pH and oxygen tension. Although most animal cells will not multiply in free suspension, certain cell lines have lost the requirement of being attached to a solid surface. These cells can be subcultured indefinitely but have some resemblance to cancer cells such as their abnormal karyotype. Certain cell lines developed from human embryonic tissue maintain their diploid character after repeated subculture and would seem to be ideal for the production of vaccines. However, strict regulations exist for viral products for human injection in that only cells taken from normal tissue and subcultured but once may be used.A microcarrier method in which cells adhere to DEAE-Sephadex beads permits a suspension culture which may be termed quaaihomogeneous. The attached cells may be retained by sedimentation or by screening as the medium is replaced. Cell debris from the original tissue is difficult to remove from microcarrier cultures; modifications of the trypsinization technique have alleviated but not solved this problem.Conditions for virus replication can be less critical than those for cell growth in that oxygen tension seems to have little influence on virus production. In cases where rate of virus production increases with specific growth rate of cells, homogeneous culture would have a valuable advantage in maintaining a high cell growth rate for a longer time. Some virus infections destroy cells, but others cause little change in cellular mteabolism except that virus is continually produced. The latter type can be conducted with a microcarrier in continuous culture with a virus titer exceeding lo7 plaque forming units per milliliter for over 50 days with Rubella-infected BHK cells.

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Production of Semliki Forest Virus from hamster kidney cells in deep culture vessels

Cited by 9 publications

References 7 publications

Factors affecting the production of foot-and-mouth disease virus in deep suspension cultures of BHK21 Clone 13 cells

Factors affecting the production of foot-and-mouth disease virus in deep suspension cultures of BHK21 Clone 13 cells

Design of a large‐scale mammalian cell, suspension culture facility

Homogeneous cultivation of animal cells for the production of virus and virus products

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