Production of recombinant enveloped structural proteins from the Chinese WSSV isolate

Abstract

The white spot syndrome virus (WSSV) is one of the deadly pathogens of penaeid shrimps and other crustaceans. The WSSV virion consists of an enveloped rod-shaped nucleocapsid enclosing a large circular double stranded DNA genome of 305 Kb with 181 open reading frames. The two major structural genes, VP19 and VP28 were amplified from the genomic DNA of Chinese isolate of WSSV and ctoned in pUCm-T vector and sub cloned in pET-30a (+) vector. The expressions of genes in E. coil (BL21) were confirmed by SDS-PAGE analysis. The clones were sequenced, submitted to the gene bank and the Xiang Shan strain of WSSV were compared with the previous reported sequence of WSSV of various regions which revealed that VP19 and VP28 gene sequences had certain differences from the sequences of similar genes of the isolate already reported. The recombinant proteins expressed, purified and characterized.

Pengklonan Gen Penyandi Viral Protein 15 (Vp-15) WSSV Dan Aplikasinya Sebagai Vaksin Rekombinan Pada Udang Windu

“…Keberhasilan pengklonan gen VP-WSSV pada vektor yang sama juga telah dilaporkan untuk VP-15, VP-19, VP-28, dan VP-35 (Seok et al, 2004). Pengklonan gen VP-WSSV telah berhasil dilakukan pada vektor pGEM-T Easy (Liu et al, 2001;Xing & Shi, 2011), pada vektor ekspresi pGEX-4T-2 (Mukhlis, 2010) dan vektor pUCm-T (Jha & Xu, 2005).…”