Production of reactive oxygen species by spermatozoa undergoing cooling, freezing, and thawing

Chatterjee, Suvro; Gagnon, Claude

doi:10.1002/mrd.1052

Cited by 448 publications

(266 citation statements)

References 38 publications

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“…Such injuries may result from oxidative stress and lipid peroxidation, due to the spermatozoa's exposure to reactive oxygen species (ROS) derived from their own metabolism (Sinha et al, 1996;Peris et al, 2007), which leads to premature capacitation (Bailey et al, 2000;Aisen et al, 2005). Therefore, the inclusion of substances with antioxidant properties in freezing extenders may prevent cryoinjuries in spermatozoa's membranes and organelles that may impair their post-thawing viability (Chatterjee and Gagnon, 2001). Many antioxidant additives have been tested in protocols for freezing ram sperm (Bucak et al, 2008;Maia et al, 2010), but due to their inconsistent results, further research in this field is justified.…”

Section: Introductionmentioning

confidence: 99%

Effect of β-mercaptoetanol and cysteine on post-thawing quality and oxidative activity of ram sperm and on the viability of vitrified sheep embryos

Pradieé

Cardoso²,

Silva³

et al. 2016

Arq. Bras. Med. Vet. Zootec.

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Effect of β-mercaptoetanol and cysteine on post-thawing quality and oxidative activity of ram sperm and on the viability of vitrified sheep embryos ABSTRACTThe effects of β-mercaptoethanol (BME) and cysteine on the viability and oxidative activity of ram sperm after thawing and on development in vitro and viability of vitrified sheep embryos were evaluated.Ejaculates from four rams were pooled and extended, composing six treatments: no antioxidants; 2mM BME; 5mM BME; 2mM BME and 5mM cysteine; 5mM BME and 5mM cysteine; and 5mM cysteine. Sperm motility, membrane and acrosome integrity, mitochondrial functionality, production of reactive oxygen species and total antioxidant capacity were similar across treatments (P>0.05). A medium with no antioxidant presented cleavage and blastocyst development rates (60.3% and 33.6%, respectively) similar (P>0.05) to those of a medium with 50μM BME and 600μM cysteine (64.3% and 36.6%, respectively). Post-thawing viability of vitrified embryos was similar between media (P>0.05). Cysteine and BME had no influence on the post-thawing viability and oxidative activity of ram sperm and on the viability of vitrified sheep embryos.

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Section: Introductionmentioning

confidence: 99%

Effect of β-mercaptoetanol and cysteine on post-thawing quality and oxidative activity of ram sperm and on the viability of vitrified sheep embryos

Pradieé

Cardoso²,

Silva³

et al. 2016

Arq. Bras. Med. Vet. Zootec.

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“…Cold shock during sperm cryopreservation is associated with oxidative stress and reactive oxygen species (ROS) generation [8]. ROS-induced damage to spermatozoa is mediated by oxidative attack of bis-allylic methylene groups of sperm phospholipid-bound polyunsaturated fatty acids (PUFAs), leading to lipid peroxidation [9,10].…”

Section: Introductionmentioning

confidence: 99%

Effect of genistein supplementation of thawing medium on characteristics of frozen human spermatozoa

Martínez-Soto¹,

DiosHourcade²,

Gutiérrez‐Adán³

et al. 2010

Asian J Androl

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In this study, we evaluated the effects of genistein supplementation of the thawing extender on frozen-thawed human semen parameters. We analyzed the effect of supplementation on sperm motility, capacitation (membrane lipid disorder), reactive oxygen species (ROS) generation, chromatin condensation and DNA damage. Using this preliminary information, it maybe possible to improve the cryopreservation process and reduce the cellular damage. We have confirmed that the isoflavone genistein (10 mmol L -1 ) has antioxidant properties on the frozen-thawed spermatozoa. This results in a decreased ROS production that shows a slight improvement in the sperm motility, and decreases the membrane lipid disorder and DNA damage caused by cryopreservation. These results suggest an effect of genistein on sperm functionality that could be of interest for assisted reproduction treatments using frozenthawed human spermatozoa, but further studies will be necessary to confirm our findings and to evaluate the possible clinical applications.

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“…With the addition of glycerol, LDL at lower concentrations (2%-6%) yielded similar post-thaw motility (46%-54%) and AMI (28)(29)(30)(31)(32)(33)(34) as that of 8% LDL (53%66% for motility and 3368 for AMI) or 10% LDL (53%69% for motility and 36610 for AMI).…”

Section: Evaluation Of Ldl In Combination With Glycerolmentioning

confidence: 89%

“…28 High levels of reactive oxygen species have been associated with cryopreservation and were postulated to be at least partially responsible for decreased sperm function after thawing. 29 Vitamin E is classified as the most potent non-enzymatic antioxidant, 30 and is one of the major membrane protectants against reactive oxygen species and lipid peroxidation. 31 More recently, many antioxidants have been used as supplements in extenders for cryopreservation of sperm with beneficial effects (e.g., Refs 32-34).…”

Section: Discussionmentioning

confidence: 99%

The role of low-density lipoprotein (LDL) and high-density lipoprotein (HDL) in comparison with whole egg yolk for sperm cryopreservation in rhesus monkeys

Dong¹,

Rodenburg²,

Hill³

et al. 2011

Asian J Androl

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Low-density lipoprotein (LDL) extracted from hen egg yolk has recently been considered to be superior to whole egg yolk in sperm cryopreservation of various animal species. Meanwhile, there was a notion that high-density lipoprotein (HDL) in egg yolk may have a negative effect on post-thaw survival. The role of LDL and HDL in sperm cryopreservation of rhesus monkeys has not been explored. The present study evaluates their effect in comparison with egg yolk with or without the addition of permeable cryoprotectant (glycerol) on sperm cryopreservation of rhesus macaques. In addition, various additives intended to change the lipid composition of LDL-sperm membrane complex have also been tested for their effectiveness in preserving post-thaw viability. Our findings indicated that LDL is the main component in egg yolk that is responsible for its protective role for sperm cryopreservation in rhesus monkeys. Regardless of the presence or absence of glycerol, the protective role of LDL is similar to that of egg yolk and we did not observe any superiority in post-thaw survival with LDL when compared to egg yolk. Modifying the lipid composition of LDL-sperm membrane complex with the addition of cholesterol, cholesterol loaded cyclodextrin and phosphatidylcholine also did not yield any improvements in post-thaw survival; while addition of methyl-b-cyclodextrin reduced post-thaw motility. HDL plays a neutral role in sperm cryopreservation of rhesus monkeys. The present study suggests that egg yolk may still hold advantages when compared with LDL as effective components in extenders for sperm cryopreservation in rhesus monkeys.

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Production of reactive oxygen species by spermatozoa undergoing cooling, freezing, and thawing

Cited by 448 publications

References 38 publications

Effect of β-mercaptoetanol and cysteine on post-thawing quality and oxidative activity of ram sperm and on the viability of vitrified sheep embryos

Effect of β-mercaptoetanol and cysteine on post-thawing quality and oxidative activity of ram sperm and on the viability of vitrified sheep embryos

Effect of genistein supplementation of thawing medium on characteristics of frozen human spermatozoa

The role of low-density lipoprotein (LDL) and high-density lipoprotein (HDL) in comparison with whole egg yolk for sperm cryopreservation in rhesus monkeys

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