Production of polygalacturonase by immobilized cells of Aspergillus niger using orange peel as inducer

Nighojkar, Sadhana; Phanse, Yashdeep; Sinha, Durganand; Nighojkar, Anand; Kumar, Anil

doi:10.1016/j.procbio.2005.12.009

Cited by 44 publications

(34 citation statements)

References 18 publications

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“…Similarly, based on a study conducted by Nighojkar et al [33], PG production by alginate immobilized A. niger in submerged culture decreased when OP concentration was above 15 g/L. Teixeira et al [34] also observed decreased exo-PG production by A. japonicus when the pectin concentration in the liquid medium was increased from 5 to 10 g/L.…”

Section: Resultsmentioning

confidence: 82%

“…Same authors also demonstrated that pectinase activity was stimulated, whereas cellulase and hemicellulase activities were inhibited by AS [5]. In another study, the same amount of (15 g/L) OP and pectin yielded similar PG activity values; however, activity was less when they were supplemented by glucose, peptone and yeast extract [33].…”

Section: Resultsmentioning

confidence: 88%

“…Rangarajan et al [49] utilized OP extract successfully as a substrate for exo-PG production in submerged culture using A. niger and reported a yield of 6,800 U/g of OP used. A. giganteus produced 48.5 U/mL PG on orange bagasse [50], while activity was below 1 U/mL with immobilized A. niger growing on OP [33]. It should be noted that, unlike most of the other studies, in this study, a single low cost substrate (OP), which was supplemented solely with a simple inorganic N source (AS), was utilized.…”

Section: Fed-batch Culturementioning

confidence: 95%

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Utilization of orange peel, a food industrial waste, in the production of exo-polygalacturonase by pellet forming Aspergillus sojae

Büyükkileci

Lahore

Tarı

2014

Bioprocess Biosyst Eng

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The production of exo-polygalacturonase (exo-PG) from orange peel (OP), a food industrial waste, using Aspergillus sojae was studied in submerged culture. A simple, low-cost, industrially significant medium formulation, composed of only OP and (NH 4 ) 2 SO 4 (AS) was developed. At an inoculum size of 2.8 9 10 3 spores/mL, growth was in the form of pellets, which provided better mixing of the culture broth and higher exo-PG activity. These pellets were successfully used as an inoculum for bioreactors and 173.0 U/mL exo-PG was produced. Fed-batch cultivation further enhanced the exo-PG activity to 244.0 U/mL in 127.5 h. The final morphology in the form of pellets is significant to industrial fermentation easing the subsequent downstream processing. Furthermore, the low pH trend obtained during this fermentation serves an advantage to fungal fermentations prone to contamination problems. As a result, an economical exo-PG production process was defined utilizing a food industrial byproduct and producing high amount of enzyme.

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Section: Resultsmentioning

confidence: 82%

Section: Resultsmentioning

confidence: 88%

Section: Fed-batch Culturementioning

confidence: 95%

See 1 more Smart Citation

Utilization of orange peel, a food industrial waste, in the production of exo-polygalacturonase by pellet forming Aspergillus sojae

Büyükkileci

Lahore

Tarı

2014

Bioprocess Biosyst Eng

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“…Fungi are known to be a good source of PG enzyme. A number of Aspergillus, Rhizopus and Penicillum species are used for the large-scale production of PG enzymes [28,29]. Another potential candidate for PG enzyme production is Aspergillus sojae, which is known as the organism used in the production of koji, a characteristic Japanese food product.…”

Section: Introductionmentioning

confidence: 99%

Modeling of polygalacturonase enzyme activity and biomass production by Aspergillus sojae ATCC 20235

Tokatlı

Tarı

Ünlütürk

et al. 2009

J Ind Microbiol Biotechnol

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Aspergillus sojae, which is used in the making of koji, a characteristic Japanese food, is a potential candidate for the production of polygalacturonase (PG) enzyme, which of a major industrial significance. In this study, fermentation data of an A. sojae system were modeled by multiple linear regression (MLR) and artificial neural network (ANN) approaches to estimate PG activity and biomass. Nutrient concentrations, agitation speed, inoculum ratio and final pH of the fermentation medium were used as the inputs of the system. In addition to nutrient conditions, the final pH of the fermentation medium was also shown to be an effective parameter in the estimation of biomass concentration. The ANN parameters, such as number of hidden neurons, epochs and learning rate, were determined using a statistical approach. In the determination of network architecture, a cross-validation technique was used to test the ANN models. Goodness-offit of the regression and ANN models was measured by the R 2 of cross-validated data and squared error of prediction. The PG activity and biomass were modeled with a 5-2-1 and 5-9-1 network topology, respectively. The models predicted enzyme activity with an R 2 of 0.84 and biomass with an R 2 value of 0.83, whereas the regression models predicted enzyme activity with an R 2 of 0.84 and biomass with an R 2 of 0.69.

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“…The inoculum size (2 ml) was found to be most effective for pectinase production (Table 3). Optimum inoculum size is important for fermentation [15]. Lactose as inducer, gelatin as organic nitrogen source and ammonium nitrate as inorganic nitrogen source yielded high enzyme titres.…”

Section: Resultsmentioning

confidence: 99%

Solid-state fermentation for production of Pectic enzymes by Aspergillus niger

Baig¹,

Apastambh²

2017

J Microbiol Biotech Res

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Production of polygalacturonase by immobilized cells of Aspergillus niger using orange peel as inducer

Cited by 44 publications

References 18 publications

Utilization of orange peel, a food industrial waste, in the production of exo-polygalacturonase by pellet forming Aspergillus sojae

Utilization of orange peel, a food industrial waste, in the production of exo-polygalacturonase by pellet forming Aspergillus sojae

Modeling of polygalacturonase enzyme activity and biomass production by Aspergillus sojae ATCC 20235

Solid-state fermentation for production of Pectic enzymes by Aspergillus niger

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